Duration of Effect of Acidform Gel on Vaginal pH

NCT ID: NCT02693418

Last Updated: 2020-11-20

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

105 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-08-24

Study Completion Date

2016-12-12

Brief Summary

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The objective of this study was to determine the magnitude and duration of the reduction in pH (acidification) following a single dose of Acidform vaginal gel containing 3, 4, or 5 g compared with hydroxyethylcellulose placebo gel (4 g) or no treatment. Vaginal pH was measured 1 h, 6 h, 12 h, 24 h, then once daily for 7 days. In addition, the participants were assessed for an exploratory endpoint to the determine effect of one dose of Acidform gel (3, 4 or 5 g) or placebo gel (4 g) or no treatment, on asymptomatic bacterial vaginosis (BV).

Detailed Description

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One hundred healthy volunteer women, 20 per each treatment arm, were treated with either Intravaginal Acidform Gel(IVAG), 5 g dose (GROUP A), Acidform gel, 4 g dose (GROUP B), Acidform gel, 3 g dose (GROUP C), Universal Placebo Gel (UPG), 4 g (GROUP D) or no treatment (GROUP E). For GROUPS A, B, C and D, treatment is defined as speculum exam plus instillation of Investigational Product (IP) or placebo gel. For GROUP E (control), no treatment is defined as speculum exam and no gel instillation.

At least 15 women in each group were of either African American or Hispanic ethnicity. Routine screening tests were performed on admission; and subjects assessed for BV via vaginal swabs obtained for grading by Amsel criteria.

A direct vaginal pH reading by research staff- was obtained before the speculum exam, as well as one hour, and six hours post-treatment (Day 0). At the one hour and six hour post treatment time points, the direct vaginal pH readings were taken on specimens collected from two different positions in the vagina, in case of incomplete distribution of the IP soon after administration. Both readings are included as data points. At the six hour timepoint, subjects were trained on self-collecting vaginal swabs and performing the vaginal pH test. At 12 hours post-treatment, subjects performed the vaginal pH test themselves using self-obtained swabs, and record their results for clinician review.

Subjects stayed overnight in the domiciliary unit, and vaginal pH and Amsel criteria was measured again by research staff at 24 (+/- 2) hours post-treatment before discharge on Day 1. The subjects were discharged with the appropriate pH testing supplies and diary. All women agreed to abstain from sexual intercourse, douching and use of any intravaginally applied products or devices until after their final study visit on Day 7.

Subjects measured their vaginal pH at 24 (+/-2) hour intervals for 5 days (Days 2-6) as outpatients and recorded the pH test results, and any change in vaginal comfort, in a provided diary. Subjects also recorded any activities engaged in from the abstinence criteria in the study exclusion list, if applicable, each day. On Day 7, subjects returned to the clinic with their diaries, had their vaginal pH and Amsel criteria determined by the clinic staff, and queried as to any vaginal discomfort (vaginal comfort assessment) over the course of the study, as self-recorded in their diaries.

Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Acidform Gel, Group A

Administration of a single vaginal dose of Acidform gel (5 g)

Group Type EXPERIMENTAL

Acidform 5 g

Intervention Type DRUG

Effect of 5 g vaginally administered Acidform on pH over 7 days

Acidform Gel, Group B

Administration of a single vaginal dose of Acidform gel (4 g)

Group Type EXPERIMENTAL

Acidform 4 g

Intervention Type DRUG

Effect of 4 g vaginally administered Acidform on pH over 7 days

Acidform Gel, Group C

Administration of a single vaginal dose of Acidform gel (3 g)

Group Type EXPERIMENTAL

Acidform 3 g

Intervention Type DRUG

Effect of 3 g vaginally administered Acidform on pH over 7 days

Placebo Gel, Group D

Administration of a single dose of hydroxyethylcellulose (HEC) placebo gel (4 g)

Group Type PLACEBO_COMPARATOR

Placebo 4 g

Intervention Type DRUG

Effect of 4 g of vaginally administered HEC placebo gel on pH over 7 days

No intervention, Group E

No vaginal product administered

Group Type NO_INTERVENTION

No interventions assigned to this group

Interventions

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Acidform 5 g

Effect of 5 g vaginally administered Acidform on pH over 7 days

Intervention Type DRUG

Placebo 4 g

Effect of 4 g of vaginally administered HEC placebo gel on pH over 7 days

Intervention Type DRUG

Acidform 4 g

Effect of 4 g vaginally administered Acidform on pH over 7 days

Intervention Type DRUG

Acidform 3 g

Effect of 3 g vaginally administered Acidform on pH over 7 days

Intervention Type DRUG

Other Intervention Names

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Natrasol 250 HX Pharm

Eligibility Criteria

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Inclusion Criteria

1. Healthy female subjects between 18 and 45 years, inclusive
2. Ability to understand the consent process and procedures
3. Subjects agree to be available for all study visits
4. Written informed consent in accordance with institutional guidelines
5. Negative pregnancy test
6. Able and willing to comply with all study procedures
7. Have not engaged in sexual intercourse, douching or used of any form of vaginal suppository or intravaginal device for 24 hours prior to enrollment.
8. Agree to abstain from sexual intercourse, douching or any form of vaginal suppository or intravaginal device use during course of study
9. Report menstrual cycle regularity (25- to 35- day menstrual cycles)

Exclusion Criteria

1. Participation in any study with an investigational compound or device within 30 days prior to signing informed consent
2. Active drug or alcohol use or dependence that, in the opinion of the investigator, would interfere with adherence to study protocol
3. Any other medical condition(s) that, in the judgment of the investigator, might interfere with the study or require treatment that might interfere with the study
4. Family member of the investigation study staff
5. Pregnant or breast-feeding
6. Inability to provide informed consent
7. A subject with a history or expectation of noncompliance with medications or treatment protocol
8. Women with symptoms of Urinary Tract Infection (UTI) or Sexually Transmitted Infection (STI) reported or observed during examination, at screening\*.
9. Women who regularly use douches, vaginal medications or suppositories, feminine sprays, genital wipes or contraceptive spermicides, or report abnormal vaginal discharge in the past 48 hours prior to screening
10. Women who are menstruating or who would expect to menstruate during the study
11. Women who are currently using contraceptives that are directly delivered to the vaginal mucosa, such as NuvaRing
12. Any specific condition that, in the judgment of the Investigator, precludes participation because it could affect subject safety
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Clinical Research Management, Inc.

INDUSTRY

Sponsor Role collaborator

Johns Hopkins University

OTHER

Sponsor Role collaborator

MetroHealth Medical Center

OTHER

Sponsor Role collaborator

Evofem Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Ann Avery, MD

Role: PRINCIPAL_INVESTIGATOR

Metro Health Medical Center

Seema Nayak, MD

Role: PRINCIPAL_INVESTIGATOR

Johns Hopkins University

John M Griffiss, MD

Role: STUDY_DIRECTOR

Clinical Research Management, Inc.

Locations

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Johns Hopkins Bayview Medical Center

Baltimore, Maryland, United States

Site Status

Metro Health Medical Center

Cleveland, Ohio, United States

Site Status

Countries

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United States

Other Identifiers

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EVO-002

Identifier Type: -

Identifier Source: org_study_id