Trial Outcomes & Findings for Role of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) on Inflammation and Lipids (NCT NCT02670382)

NCT ID: NCT02670382

Last Updated: 2021-04-14

Results Overview

plasma levels in pg/mL

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

24 participants

Primary outcome timeframe

10 weeks

Results posted on

2021-04-14

Participant Flow

405 subjects were screened for eligibility at the Metabolic Research Unit of the Human Nutrition Research Center on Aging at Tufts Unoversity

24 subjects met the inclusion criteria and were enrolled into the study. 3 subjects dropped off during the placebo lead-in phase of the study

Participant milestones

Participant milestones
Measure
Placebo, Then EPA, Then DHA
3000 mg high oleic acid sunflower oil/day during 4-week lead-in phase; then participants received 3000 mg pure EPA for 10 weeks; then washout of 10 weeks; then pure DHA 3000 mg/day Placebo and EPA and DHA capsules provided as 750 mg/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening
Placebo, Then DHA, and Then EPA
3000 mg high oleic acid sunflower oil/day during 4-week lead-in phase; then participants received 3000 mg pure DHA for 10 weeks; then washout of 10 weeks; then pure EPA 3000 mg/day Placebo and DHA and EPA capsules provided as 750 mg/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening
Placebo lead-in Phase
STARTED
12
12
Placebo lead-in Phase
COMPLETED
10
11
Placebo lead-in Phase
NOT COMPLETED
2
1
First Active Intervention
STARTED
10
11
First Active Intervention
COMPLETED
10
11
First Active Intervention
NOT COMPLETED
0
0
Second Active Intervention
STARTED
10
11
Second Active Intervention
COMPLETED
10
11
Second Active Intervention
NOT COMPLETED
0
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Placebo, Then EPA, Then DHA
3000 mg high oleic acid sunflower oil/day during 4-week lead-in phase; then participants received 3000 mg pure EPA for 10 weeks; then washout of 10 weeks; then pure DHA 3000 mg/day Placebo and EPA and DHA capsules provided as 750 mg/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening
Placebo, Then DHA, and Then EPA
3000 mg high oleic acid sunflower oil/day during 4-week lead-in phase; then participants received 3000 mg pure DHA for 10 weeks; then washout of 10 weeks; then pure EPA 3000 mg/day Placebo and DHA and EPA capsules provided as 750 mg/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening
Placebo lead-in Phase
Withdrawal by Subject
2
1

Baseline Characteristics

Role of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) on Inflammation and Lipids

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
All Participants
n=21 Participants
Baseline characteristic, defined as end of lead-in phase
Age, Continuous
62 years
STANDARD_DEVIATION 6 • n=5 Participants
Sex: Female, Male
Female
12 Participants
n=5 Participants
Sex: Female, Male
Male
9 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
1 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
2 Participants
n=5 Participants
Race (NIH/OMB)
White
18 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
Region of Enrollment
United States
21 participants
n=5 Participants
Body mass index
32.20 kg/m^2
STANDARD_DEVIATION 6.61 • n=5 Participants
C reactive protein
6.0 microg/mL
STANDARD_DEVIATION 3.4 • n=5 Participants
Glucose
99 mg/dL
STANDARD_DEVIATION 11 • n=5 Participants
triglycerides
145 mg/dL
STANDARD_DEVIATION 51 • n=5 Participants

PRIMARY outcome

Timeframe: 10 weeks

plasma levels in pg/mL

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
3000 mg high oleic acid sunflower oil/day; 750 mg high oleic acid sunflower oil/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals during 4 week long lead-in phase. sunflower oil: 4-week lead-in
EPA Intervention
n=21 Participants
Subjects randomized to receive 3000 mg EPA/day, provided as EPA 750 mg/capsule will be instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. EPA: 10 week supplementation
DHA Intervention
n=21 Participants
Subjects randomized to 3000 mg DHA/day provided as DHA 750 mg/capsule will instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. DHA: 10 week supplementation
Interleukin-6 (IL-6)
0.96 pg/mL
Standard Deviation 0.56
1.01 pg/mL
Standard Deviation 0.51
0.99 pg/mL
Standard Deviation 0.77

PRIMARY outcome

Timeframe: 10 weeks

plasma levels in pg/mL

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
3000 mg high oleic acid sunflower oil/day; 750 mg high oleic acid sunflower oil/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals during 4 week long lead-in phase. sunflower oil: 4-week lead-in
EPA Intervention
n=21 Participants
Subjects randomized to receive 3000 mg EPA/day, provided as EPA 750 mg/capsule will be instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. EPA: 10 week supplementation
DHA Intervention
n=21 Participants
Subjects randomized to 3000 mg DHA/day provided as DHA 750 mg/capsule will instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. DHA: 10 week supplementation
Tumor Necrosis Factor Alpha (TNF-alpha)
2.38 pg/mL
Standard Deviation 0.54
2.48 pg/mL
Standard Deviation 0.64
2.47 pg/mL
Standard Deviation 0.76

PRIMARY outcome

Timeframe: 10 weeks

plasma levels in mg/dL

Outcome measures

Outcome measures
Measure
Placebo
n=21 Participants
3000 mg high oleic acid sunflower oil/day; 750 mg high oleic acid sunflower oil/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals during 4 week long lead-in phase. sunflower oil: 4-week lead-in
EPA Intervention
n=21 Participants
Subjects randomized to receive 3000 mg EPA/day, provided as EPA 750 mg/capsule will be instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. EPA: 10 week supplementation
DHA Intervention
n=21 Participants
Subjects randomized to 3000 mg DHA/day provided as DHA 750 mg/capsule will instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. DHA: 10 week supplementation
Low Density Lipoprotein Cholesterol (LDL-C)
130 mg/dL
Standard Deviation 26
136 mg/dL
Standard Deviation 26
140 mg/dL
Standard Deviation 32

Adverse Events

EPA Intervention

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

DHA Intervention

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
EPA Intervention
n=21 participants at risk
Subjects randomized to receive 3000 mg EPA/day, provided as EPA 750 mg/capsule will be instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. EPA: 10 week supplementation
DHA Intervention
n=21 participants at risk
Subjects randomized to 3000 mg DHA/day provided as DHA 750 mg/capsule will instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals for 10 weeks. DHA: 10 week supplementation
Placebo
n=21 participants at risk
3000 mg high oleic acid sunflower oil/day; 750 mg high oleic acid sunflower oil/capsule; subjects instructed to take 2 capsules by mouth in the morning and 2 in the evening with meals during 4 week long lead-in phase. sunflower oil: 4-week lead-in
Metabolism and nutrition disorders
gout
0.00%
0/21 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks
0.00%
0/21 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks
4.8%
1/21 • Number of events 1 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks
Cardiac disorders
hypertension
0.00%
0/21 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks
0.00%
0/21 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks
4.8%
1/21 • Number of events 1 • adeverse events were collected during the participation of study subjects, equivalent to 34 weeks

Additional Information

Dr. Stefania Lamon-Fava, Scientist I

Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University

Phone: 617-556-3105

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place