Myeloid to Adipocyte Transdifferentiation in Human Cells

NCT ID: NCT02654925

Last Updated: 2021-06-24

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 59 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2016-03-31
• Study Completion Date: 2021-06-15

Brief Summary

In order to design new programs to help prevent weight gain, promote successful and sustainable weight loss, and help treat diseases related to obesity, the investigators need a better understanding of why accumulating fat in certain regions of the body is bad for health. It is known that not all fat cells are the same, but it was recently discovered that some fat cells may arise from stem cells that come from the bone marrow (bone marrow progenitors), a previously unrecognized origin. This discovery has been paradigm shifting, because dogma has long held that all white fat cells arise from fat tissue resident mesenchymal stem cells. This is also important because fat cells arising from the bone marrow lineage may be linked to worse health outcomes. The aim of this study is to determine if cells that were not previously believed to contribute to fat generation in humans are indeed capable of becoming fat cells. To answer this question the investigators will take samples of cells from the blood and the fat tissue of younger and older men and women, isolate the cells that came from the bone marrow and grow them in culture to determine if they will turn into fat cells. The results of this investigation may reveal potential mechanistic targets for future therapies to reduce the negative health outcomes associated with obesity related chronic diseases.

Conditions

Obesity

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: OTHER

Study Groups

Young Men

men 21-40 years of age

Adipose Tissue Biopsy

Intervention Type: PROCEDURE

Young Women

women 21-40 years of age; young women will be premenopausal and eumenorrheic, not on hormonal contraceptive therapy.

Adipose Tissue Biopsy

Intervention Type: PROCEDURE

Older Men

men 55-100 years of age

Adipose Tissue Biopsy

Intervention Type: PROCEDURE

Older Women

women 55-100 years of age; older women will be postmenopausal who are at least 12 months past the final menstrual period (FMP).

Adipose Tissue Biopsy

Intervention Type: PROCEDURE

Interventions

Adipose Tissue Biopsy

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

• Men and women age 21-40 y or 55-100 y;
• Older women will be postmenopausal who are at least 12 months past the final menstrual period (FMP);
• Young women will be premenopausal and eumenorrheic, not on hormonal contraceptive therapy;
• Body Mass Index (BMI) 22-35 kg/m2;
• Non-active to moderately active (vigorous exercise ≤ 3 days/wk lasting < 30 min).

Exclusion Criteria

• Immunosuppression therapy;
• On blood thinners, aspirin, or NSAIDs that cannot be withheld for the biopsy;
• Prior history of allergies to local anesthetics;
• Current use of hormones, or glucose lowering medication;
• Currently engaged in a vigorous exercise or diet program;
• Currently gaining or losing weight or using weight loss drugs;
• Type 2 diabetes (past/current diagnosis or treatment);
• Diagnosis of uncontrolled metabolic disorders (e.g., thyroid);
• History of severe obesity BMI ≥ 40 or significant weight loss (≥ 50 lbs);
• Postmenopausal - hormonal menopausal therapy use currently or in the previous 6 months;
• Premenopausal - irregular menstrual cycles defined as 2 or more missed cycles in the previous year, pregnant or planning to become pregnant during the study timeline (urine pregnancy test will be performed before DEXA [dual energy x-ray absorptiometry] scan is completed).

• Minimum Eligible Age: 21 Years
• Maximum Eligible Age: 100 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of Colorado, Denver

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Kathleen M Gavin, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Colorado Denver Anschutz Medical Campus

Locations

University of Colorado Anschutz Medical Campus

Aurora, Colorado, United States

Countries

United States

Other Identifiers

UL1TR001082

Identifier Type: NIH

Identifier Source: secondary_id

View Link

15-1779

Identifier Type: -

Identifier Source: org_study_id