Vitamin D and Microbiota in Cystic Fibrosis

NCT ID: NCT02589444

Last Updated: 2017-06-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

41 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-12-31

Study Completion Date

2017-04-30

Brief Summary

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The objective of this study is to assess the effects of a high-dose vitamin D3 on the composition of gut and lung microbiota in adolescents and adults with cystic fibrosis who are vitamin D deficient.

Detailed Description

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Monocentric, double-blind, randomized, placebo-controlled, interventional pilot study to investigate the beneficial effects of high dose vitamin D supplementation on gut and lung microbiota in patients with cystic fibrosis who are vitamin D insufficient.

Conditions

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Vitamin D Deficiency Cystic Fibrosis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

SUPPORTIVE_CARE

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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Participants with vitamin D deficiency - treatment group

Participants with 25-hydroxyvitamin D (25(OH)D) ≤30 ng/mL taking oral high-dose vitamin D3 (50,000 IU) once a week and providing stool and sputum sample at screening and 3 months after screening.

Group Type EXPERIMENTAL

High-Dose Vitamin D3

Intervention Type DIETARY_SUPPLEMENT

50,000 IU of oral vitamin D3 once a week (the standard of care for repletion of vitamin D status by the Cystic fibrosis Foundation)

Stool Sample

Intervention Type OTHER

Participants will be asked to provide a stool sample in a collection container for analysis of stool microbiota. This will be done upon enrollment (baseline) and at 3 month follow-up.

Sputum Sample

Intervention Type OTHER

Participants will be asked to collect their sputum (the thick mucus or phlegm that is expelled from the lower respiratory tract through coughing) into a kit.

This will be done upon enrollment (baseline) and at 3 month follow-up.

Blood draw

Intervention Type PROCEDURE

Participants will be asked to provide 30 ml of blood collected via a blood draw to measure 25 (OH)D serum concentration and other nutrient markers related to vitamin D including Parathyroid hormone, fibroblast growth factor-23, vitamin D binding protein and markers of immune system/inflammation. This will be done at baseline and at 3 months follow up.

Participants with vitamin D deficiency - placebo group

Participants with 25-hydroxyvitamin D (25(OH)D) concentrations ≤30 ng/mL taking a sham comparator (placebo) once a week and providing stool sample and sputum sample at screening and 3 months after screening.

Group Type SHAM_COMPARATOR

Stool Sample

Intervention Type OTHER

Participants will be asked to provide a stool sample in a collection container for analysis of stool microbiota. This will be done upon enrollment (baseline) and at 3 month follow-up.

Sputum Sample

Intervention Type OTHER

Participants will be asked to collect their sputum (the thick mucus or phlegm that is expelled from the lower respiratory tract through coughing) into a kit.

This will be done upon enrollment (baseline) and at 3 month follow-up.

Sham Comparator

Intervention Type OTHER

A placebo capsule taken once a week (manufactured by the same company that makes the Vitamin D supplement).

Blood draw

Intervention Type PROCEDURE

Participants will be asked to provide 30 ml of blood collected via a blood draw to measure 25 (OH)D serum concentration and other nutrient markers related to vitamin D including Parathyroid hormone, fibroblast growth factor-23, vitamin D binding protein and markers of immune system/inflammation. This will be done at baseline and at 3 months follow up.

Participants without vitamin D deficiency

Participants with 25-hydroxyvitamin D (25(OH)D) concentrations \> 30 ng/mL with no intervention and providing stool sample and sputum sample at screening and 3 months after screening.

Group Type OTHER

Stool Sample

Intervention Type OTHER

Participants will be asked to provide a stool sample in a collection container for analysis of stool microbiota. This will be done upon enrollment (baseline) and at 3 month follow-up.

Sputum Sample

Intervention Type OTHER

Participants will be asked to collect their sputum (the thick mucus or phlegm that is expelled from the lower respiratory tract through coughing) into a kit.

This will be done upon enrollment (baseline) and at 3 month follow-up.

Blood draw

Intervention Type PROCEDURE

Participants will be asked to provide 30 ml of blood collected via a blood draw to measure 25 (OH)D serum concentration and other nutrient markers related to vitamin D including Parathyroid hormone, fibroblast growth factor-23, vitamin D binding protein and markers of immune system/inflammation. This will be done at baseline and at 3 months follow up.

Interventions

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High-Dose Vitamin D3

50,000 IU of oral vitamin D3 once a week (the standard of care for repletion of vitamin D status by the Cystic fibrosis Foundation)

Intervention Type DIETARY_SUPPLEMENT

Stool Sample

Participants will be asked to provide a stool sample in a collection container for analysis of stool microbiota. This will be done upon enrollment (baseline) and at 3 month follow-up.

Intervention Type OTHER

Sputum Sample

Participants will be asked to collect their sputum (the thick mucus or phlegm that is expelled from the lower respiratory tract through coughing) into a kit.

This will be done upon enrollment (baseline) and at 3 month follow-up.

Intervention Type OTHER

Sham Comparator

A placebo capsule taken once a week (manufactured by the same company that makes the Vitamin D supplement).

Intervention Type OTHER

Blood draw

Participants will be asked to provide 30 ml of blood collected via a blood draw to measure 25 (OH)D serum concentration and other nutrient markers related to vitamin D including Parathyroid hormone, fibroblast growth factor-23, vitamin D binding protein and markers of immune system/inflammation. This will be done at baseline and at 3 months follow up.

Intervention Type PROCEDURE

Other Intervention Names

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Cholecalciferol Placebo

Eligibility Criteria

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Inclusion Criteria

* Presenting to the Cystic fibrosis clinic for routine follow up of cystic fibrosis
* Serum 25(OH)D concentrations obtained within 2 months of enrollment
* Able to tolerate oral medications

Exclusion Criteria

* Inability to obtain or declined informed consent from the subject and/or legally authorized representative
* Pregnancy or plans to become pregnant in the next 3 months
* History of disorders associated with hypercalcemia including parathyroid disease
* Current hypercalcemia (albumin-corrected serum calcium \>10.8 mg/dL or ionized calcium \>5.2 mg/dL)
* History of nephrolithiasis with active symptoms within the past two years
* Chronic kidney disease worse than stage III (\<60 ml/min)
* Current significant hepatic dysfunction total bilirubin \> 2.5 mg/dL with direct bilirubin \> 1.0 mg/dL
* Current use of cytotoxic or immunosuppressive drugs
* History of AIDS
* History of illicit drug abuse (defined as history of enrollment into a drug rehabilitation program or hospital visits due to drug use within the past 3 years or any use of the following drugs in the past 6 months (cocaine, opiates, amphetamines, marijuana) or any positive toxicology screen for (cocaine, opiates, amphetamines, marijuana)
* Too ill to participate in study based on investigator's or study team's opinion
* Current enrollment in another intervention trial
* In addition we amended our study with three additional criteria 11) systemic antibiotic use in the last 4 weeks, 12) use of probiotics and, 13) inflammatory bowel disease, four months after the start of the study and after 12 subjects were randomized, as we considered that these factors may also influence our study endpoints. Of the 12 subjects who were randomized, only 4 would have been excluded.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Emory University

OTHER

Sponsor Role lead

Responsible Party

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Vin Tangpricha, MD, PH.D

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Vin Tangpricha, MD/PhD

Role: PRINCIPAL_INVESTIGATOR

Emory University

Locations

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Emory Clinic

Atlanta, Georgia, United States

Site Status

Countries

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United States

References

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Kanhere M, He J, Chassaing B, Ziegler TR, Alvarez JA, Ivie EA, Hao L, Hanfelt J, Gewirtz AT, Tangpricha V. Bolus Weekly Vitamin D3 Supplementation Impacts Gut and Airway Microbiota in Adults With Cystic Fibrosis: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial. J Clin Endocrinol Metab. 2018 Feb 1;103(2):564-574. doi: 10.1210/jc.2017-01983.

Reference Type DERIVED
PMID: 29161417 (View on PubMed)

Other Identifiers

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IRB00083796

Identifier Type: -

Identifier Source: org_study_id

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