Proteomic Analysis of Mononuclear Cells After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice
NCT ID: NCT02587507
Last Updated: 2016-07-29
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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COMPLETED
NA
12 participants
INTERVENTIONAL
2015-06-30
2015-10-31
Brief Summary
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Detailed Description
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A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.
OBJECTIVES:
To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.
DESIGN AND METHODOLOGY:
Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.
Conditions
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Study Design
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RANDOMIZED
CROSSOVER
BASIC_SCIENCE
NONE
Study Groups
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Water
The subjects will ingest the HFHC meal with 500mL of water.
Water
Individuals will consume the HFHC meal with 500mL of water.
Orange juice
The subjects will ingest the HFHC meal with 500mL of orange juice.
Orange juice
Individuals will consume the HFHC meal with 500mL of orange juice.
Water with glucose
The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice).
Water with glucose
Individuals will consume the HFHC meal with 500mL of water with glucose.
Interventions
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Orange juice
Individuals will consume the HFHC meal with 500mL of orange juice.
Water
Individuals will consume the HFHC meal with 500mL of water.
Water with glucose
Individuals will consume the HFHC meal with 500mL of water with glucose.
Eligibility Criteria
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Inclusion Criteria
* normal weight
* non-vegetarian
Exclusion Criteria
* alcohol consumption,
* intense physical exercise
* pregnant or lactating
* overweight
* obese
* drug abuse
* any disease such as diabetes, inflammatory conditions.
20 Years
35 Years
ALL
Yes
Sponsors
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The Scripps Research Institute
OTHER
University of Sao Paulo
OTHER
Responsible Party
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Daniela Chaves
PhD
Principal Investigators
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Daniela FS Chaves, PhD
Role: PRINCIPAL_INVESTIGATOR
University of Sao Paulo
References
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Ghanim H, Sia CL, Upadhyay M, Korzeniewski K, Viswanathan P, Abuaysheh S, Mohanty P, Dandona P. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010 Apr;91(4):940-9. doi: 10.3945/ajcn.2009.28584. Epub 2010 Mar 3.
Coelho RC, Hermsdorff HH, Bressan J. Anti-inflammatory properties of orange juice: possible favorable molecular and metabolic effects. Plant Foods Hum Nutr. 2013 Mar;68(1):1-10. doi: 10.1007/s11130-013-0343-3.
Chaves DFS, Carvalho PC, Brasili E, Rogero MM, Hassimotto NA, Diedrich JK, Moresco JJ, Yates JR 3rd, Lajolo FM. Proteomic Analysis of Peripheral Blood Mononuclear Cells after a High-Fat, High-Carbohydrate Meal with Orange Juice. J Proteome Res. 2017 Nov 3;16(11):4086-4092. doi: 10.1021/acs.jproteome.7b00476.
Other Identifiers
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TMTMNC
Identifier Type: -
Identifier Source: org_study_id