Trial Outcomes & Findings for Comparative the Effect of Metformin and Acupuncture on Weight Loss and Insulin Sensitivity (NCT NCT02438540)

NCT ID: NCT02438540

Last Updated: 2016-12-08

Results Overview

The effect of Metformin and acupuncture combined therapy on weight loss (Change from baseline in body weight), body weight was measured while the subjects were dressed in light clothing after an overnight fasting and by a standard scale to an accuracy of +/-0.1 kg. All measures were recorded by one assessment, at baseline before the first time treatment, and before the last time treatment at week 3.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

43 participants

Primary outcome timeframe

baseline, week 3

Results posted on

2016-12-08

Participant Flow

43 participants took part in trial. 4 of them were excluded from the trial according to including and excluding criteria. At last, 39 participants were distributed by permuted-block randomization.

Participant milestones

Participant milestones
Measure
Metformin & Acupuncture
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
Metformin 500 mg (one/two/three times per day) to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and electro acupuncture (EA) machine was switched off during 30 minutes of therapeutic time. And ear acupuncture was just used sticky layers without seeds. All placebo treatments used for 30 minutes, 10 times, every other day, for 3 weeks.
Overall Study
STARTED
19
20
Overall Study
COMPLETED
19
20
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Comparative the Effect of Metformin and Acupuncture on Weight Loss and Insulin Sensitivity

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg, to control their diabetes during the period of this study as previously (with different therapeutic dosage) and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg, to control their diabetes during the period of this study as previously (with different therapeutic dosage) and placebo ( for acupuncture treatment including electro body acupuncture and Auricular acupuncture), needling not in right acupoints (for those points that were located in the abdomen, needles were inserted 0.3 cm laterally from the real location and the needling was maximally superficial. Those points that were located on other parts of the body, needles were inserted 0.5 cm up and 0.5 cm laterally from the real location and needling was superficial as well. Electric lines were connected with some of the needles same way they were connected in another case group. EA machine was switched off during 30 minutes of therapeutic time. Ear acupuncture was used on the same location as in the case group however we just used sticky layers without seeds), for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo (for acupuncture including electro
Total
n=39 Participants
Total of all reporting groups
Age, Customized
Mean age
42.2 years
STANDARD_DEVIATION 3.5 • n=5 Participants
40.5 years
STANDARD_DEVIATION 2.6 • n=7 Participants
41.3 years
STANDARD_DEVIATION 3.0 • n=5 Participants
Sex: Female, Male
Female
11 Participants
n=5 Participants
12 Participants
n=7 Participants
23 Participants
n=5 Participants
Sex: Female, Male
Male
8 Participants
n=5 Participants
8 Participants
n=7 Participants
16 Participants
n=5 Participants

PRIMARY outcome

Timeframe: baseline, week 3

The effect of Metformin and acupuncture combined therapy on weight loss (Change from baseline in body weight), body weight was measured while the subjects were dressed in light clothing after an overnight fasting and by a standard scale to an accuracy of +/-0.1 kg. All measures were recorded by one assessment, at baseline before the first time treatment, and before the last time treatment at week 3.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Body Weight
-4.1 kg
Standard Deviation 1.1
-1.5 kg
Standard Deviation 0.3

PRIMARY outcome

Timeframe: baseline, week 2, week 3

changes FBS, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Fasting Blood Sugar (FBS)
FBS blood concentration at the 2th sample, week 2
6.44 mmol/L
Standard Deviation 0.5
6.55 mmol/L
Standard Deviation 0.5
Fasting Blood Sugar (FBS)
FBS blood concentration at the 3th sample, week 3
6.12 mmol/L
Standard Deviation 0.5
6.54 mmol/L
Standard Deviation 0.5
Fasting Blood Sugar (FBS)
FBS blood concentration at the 1th sample, baselin
6.65 mmol/L
Standard Deviation 0.6
6.67 mmol/L
Standard Deviation 0.5

PRIMARY outcome

Timeframe: baseline, week 2, week 3

change of FINS,Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Fasting Insulin (FINS)
FINS blood level at the 1th sample, baseline
14.47 µIU/ml
Standard Deviation 0.8
14.45 µIU/ml
Standard Deviation 0.7
Fasting Insulin (FINS)
FINS blood level at the 2th sample, week 2
12.92 µIU/ml
Standard Deviation 0.7
13.75 µIU/ml
Standard Deviation 0.7
Fasting Insulin (FINS)
FINS blood level at the 3th sample, week 3
9.91 µIU/ml
Standard Deviation 0.7
13.6 µIU/ml
Standard Deviation 0.8

PRIMARY outcome

Timeframe: baseline, week 2, week 3

IL-6 changes, IL-6 were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Interleukin-6 (IL-6)
IL-6 blood level at the 1th sample, baseline
1.44 Pg/dl
Standard Deviation 0.03
1.43 Pg/dl
Standard Deviation 0.02
Interleukin-6 (IL-6)
IL-6 blood level at the 2th sample, week 2
1.30 Pg/dl
Standard Deviation 0.04
1.41 Pg/dl
Standard Deviation 0.02
Interleukin-6 (IL-6)
IL-6 blood level at the 3th sample, week 3
1.09 Pg/dl
Standard Deviation 0.05
1.37 Pg/dl
Standard Deviation 0.04

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Blood markers changes in TNF α, were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Tumor Necrosis Factor-α (TNF-α)
TNF-α blood level at the 1th sample, baseline
1.44 Pg/ml
Standard Deviation 0.02
1.44 Pg/ml
Standard Deviation 0.02
Tumor Necrosis Factor-α (TNF-α)
TNF-α blood level at the 2th sample, week 2
1.42 Pg/ml
Standard Deviation 0.04
1.43 Pg/ml
Standard Deviation 0.02
Tumor Necrosis Factor-α (TNF-α)
TNF-α blood level at the 3th sample, week 3
1.41 Pg/ml
Standard Deviation 0.05
1.43 Pg/ml
Standard Deviation 0.02

PRIMARY outcome

Timeframe: baseline, week 2, week 3

CRP blood markers changes; Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
C-reaction Protein (CRP)
CRP blood level at the 1th sample, baseline
0.60 mg/dl
Standard Deviation 0.03
0.59 mg/dl
Standard Deviation 0.03
C-reaction Protein (CRP)
CRP blood level at the 2th sample, week 2
0.59 mg/dl
Standard Deviation 0.03
0.58 mg/dl
Standard Deviation 0.03
C-reaction Protein (CRP)
CRP blood level at the 3th sample, week 3
0.57 mg/dl
Standard Deviation 0.03
0.57 mg/dl
Standard Deviation 0.03

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Free Fatty Acids (FFAs)
FFAs blood level at the 1th sample, baseline
0.72 mmol/L
Standard Deviation 0.04
0.72 mmol/L
Standard Deviation 0.04
Free Fatty Acids (FFAs)
FFAs blood level at the 2th sample, week 2
0.61 mmol/L
Standard Deviation 0.03
0.70 mmol/L
Standard Deviation 0
Free Fatty Acids (FFAs)
FFAs blood level at the 3th sample, week 3
0.51 mmol/L
Standard Deviation 0.03
0.63 mmol/L
Standard Deviation 0.04

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Triglyceride (TG)
TG blood level at the 2th sample, week 2
2.44 mmol/L
Standard Deviation 0.6
2.69 mmol/L
Standard Deviation 0.7
Triglyceride (TG)
TG blood level at the 1th sample, baseline
2.59 mmol/L
Standard Deviation 0.6
2.69 mmol/L
Standard Deviation 0.7
Triglyceride (TG)
TG blood level at the 3th sample, week 3
2.24 mmol/L
Standard Deviation 0.5
2.65 mmol/L
Standard Deviation 0.7

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Low Density Lipoprotein Cholesterol (LDLc)
LDLc blood level at the 1th sample, baseline
4.05 mmol/L
Standard Deviation 0.5
4.08 mmol/L
Standard Deviation 0.5
Low Density Lipoprotein Cholesterol (LDLc)
LDLc blood level at the 2th sample, week 2
3.88 mmol/L
Standard Deviation 0.5
4.0 mmol/L
Standard Deviation 0.5
Low Density Lipoprotein Cholesterol (LDLc)
LDLc blood level at the 3th sample, week 3
3.68 mmol/L
Standard Deviation 0.5
3.95 mmol/L
Standard Deviation 0.5

PRIMARY outcome

Timeframe: baseline, week 2, week 3

HDLc changes, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
High Density Lipoprotein Cholesterol (HDLc)
HDLc blood level at the 1th sample, baseline
1.08 mmol/L
Standard Deviation 0.1
1.16 mmol/L
Standard Deviation 0.1
High Density Lipoprotein Cholesterol (HDLc)
HDLc blood level at the 2th sample, week 2
1.20 mmol/L
Standard Deviation 0.1
1.21 mmol/L
Standard Deviation 0.1
High Density Lipoprotein Cholesterol (HDLc)
HDLc blood level at the 3th sample, week 3
1.29 mmol/L
Standard Deviation 0.1
1.25 mmol/L
Standard Deviation 0.1

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Changes in ceramides blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Ceramides
ceramides blood level at the 1th sample, baseline
8.06 g/dl
Standard Deviation 0.2
7.92 g/dl
Standard Deviation 0.2
Ceramides
ceramides blood level at the 2th sample, week 2
7.24 g/dl
Standard Deviation 0.3
7.45 g/dl
Standard Deviation 0.2
Ceramides
ceramides blood level at the 3th sample, week 3
6.06 g/dl
Standard Deviation 0.3
7.18 g/dl
Standard Deviation 0.2

PRIMARY outcome

Timeframe: baseline, week 2, week 3

changes in Leptin blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Leptin
Leptin blood level at the 1th sample, baseline
13.32 ng/ml
Standard Deviation 0.2
13.35 ng/ml
Standard Deviation 0.2
Leptin
Leptin blood level at the 2th sample, week 2
11.58 ng/ml
Standard Deviation 0.4
12.67 ng/ml
Standard Deviation 0.4
Leptin
Leptin blood level at the 3th sample, week 3
10.84 ng/ml
Standard Deviation 0.4
12.60 ng/ml
Standard Deviation 0.4

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Changes in Adiponectin blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Adiponectin
Adiponectin blood level at the 2th sample, week 2
8.26 µg/ml
Standard Deviation 0.2
7.58 µg/ml
Standard Deviation 0.3
Adiponectin
Adiponectin blood level at the baseline
7.47 µg/ml
Standard Deviation 0.3
7.48 µg/ml
Standard Deviation 0.2
Adiponectin
Adiponectin blood level at the 3th sample, week 3
8.73 µg/ml
Standard Deviation 0.2
7.73 µg/ml
Standard Deviation 0.3

PRIMARY outcome

Timeframe: baseline, week 2, week 3

changes in GLP-1 blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Glucagon-like Peptide-1 (GLP-1)
GLP-1 blood level at the 1th sample, baseline
3.77 mmol/L
Standard Deviation 0.2
3.75 mmol/L
Standard Deviation 0.2
Glucagon-like Peptide-1 (GLP-1)
GLP-1 blood level at the 2th sample, week 2
3.49 mmol/L
Standard Deviation 0.2
3.50 mmol/L
Standard Deviation 0.2
Glucagon-like Peptide-1 (GLP-1)
GLP-1 blood level at the 3th sample, week 3
3.21 mmol/L
Standard Deviation 0.1
3.43 mmol/L
Standard Deviation 0.2

PRIMARY outcome

Timeframe: baseline, week 2, week 3

Changes in Resistin blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Resistin
Resistin blood level at the 1th sample, baseline
7.43 ng/ml
Standard Deviation 0.2
7.36 ng/ml
Standard Deviation 0.2
Resistin
Resistin blood level at the 2th sample, week 2
6.34 ng/ml
Standard Deviation 0.3
6.76 ng/ml
Standard Deviation 0.2
Resistin
Resistin blood level at the 3th sample, week 3
5.24 ng/ml
Standard Deviation 0.3
6.54 ng/ml
Standard Deviation 0.1

PRIMARY outcome

Timeframe: baseline, week 2, week 3

changes in Serotonin blood level, Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Serotonin
Serotonin blood level at the 1th sample, baseline
130.4 ng/ml
Standard Deviation 3
128 ng/ml
Standard Deviation 2
Serotonin
Serotonin blood level at the 2th sample, week 2
144.9 ng/ml
Standard Deviation 2
135.1 ng/ml
Standard Deviation 2
Serotonin
Serotonin blood level at the 3th sample, week 3
170.5 ng/ml
Standard Deviation 3
147 ng/ml
Standard Deviation 3

SECONDARY outcome

Timeframe: baseline, week 3

change of BMI. Body height was measured to an accuracy of +/-0.1cm. BMI was calculated by dividing weight (kg) into height (squared m²).

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
Body Mass Index (BMI)
BMI blood level at the 3th sample, week 3
26.2 Kg/m²
Standard Deviation 2.4
27.7 Kg/m²
Standard Deviation 1.6
Body Mass Index (BMI)
BMI blood level at the 1th sample, baseline
27.6 Kg/m²
Standard Deviation 2.5
28.2 Kg/m²
Standard Deviation 1.6

SECONDARY outcome

Timeframe: baseline, week 2, week 3

Changes of IR was calculated by the homeostasis model (HOMA-IR), proposed by Matthews et al. HOMA-IR = (fasting insulin (mmol/L) × fasting glucose (µIU/ml))/22•5. Blood markers were calculated by measuring them after drawing 10 ml of blood from cubital vein in each patient, after 8 hours of overnight fasting and before treatment; Blood was collected three times during the study (at the beginning, at the 5th time, and at the end), from groups, using standard range and ELISA diagnostic kits (Biomatik USA LLC, www.biomatik.com), and clinical assessments and measurements undertaken after 3 weeks. Readings/assessment was performed at 3 weeks.

Outcome measures

Outcome measures
Measure
Metformin & Acupuncture
n=19 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and acupuncture treatment including electro body acupuncture and Auricular acupuncture for 30 minutes, 10 times, every other day, for 3 weeks. metformin acupuncture: Electro body acupuncture and auricular acupuncture
Metformin & Placebo
n=20 Participants
Metformin 500 mg (one/two/three times per day), to control their diabetes during the period of this study as previously, and placebo acupuncture treatment, needling not in right acupoints and EA machine was switched off during 30 minutes of therapeutic time. For ear acupuncture was just used sticky layers without seeds. All placebo treatment used for 30 minutes, 10 times, every other day, for 3 weeks. metformin Placebo acupuncture including placebo EA and auricular acupuncture
HOMA-IR
HOMA-IR at the 1th sample, baseline
4.25 units on a scale
Standard Deviation 0.2
4.36 units on a scale
Standard Deviation 0.6
HOMA-IR
HOMA-IR at the 2th sample, week 2
3.68 units on a scale
Standard Deviation 0.1
4.06 units on a scale
Standard Deviation 0.5
HOMA-IR
HOMA-IR at the 3th sample, week 3
2.67 units on a scale
Standard Deviation 0.1
4.01 units on a scale
Standard Deviation 0.5

Adverse Events

Metformin & Acupuncture

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Metformin & Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Amir Firouzjaei

Nanjing university of TCM

Phone: +989125397047

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place