Characterizing the Gut Microbiota Alteration Associated With Severe Acute Malnutrition

NCT ID: NCT02413905

Last Updated: 2015-04-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2014-01-31

Study Completion Date

2015-03-31

Brief Summary

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The investigators performed two case-control studies in Niger and Senegal analysing fecal microbiota to characterize the specificity of the gut microbiota alteration associated with severe acute malnutrition (SAM).

Detailed Description

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Fecal samples of cases (severe acute malnutrition (SAM) ascertained according to the WHO definition) and controls (asymptomatic healthy children ascertained by clinical and anthropometric criteria) were analysed by v3v4 16S rRNA sequencing, redox and pH measurement and using specific polymerase chain reaction targeting Methanobrevibacter smithii and Bacteroides thetaiotaomicron. Based on the literature and oxydative stress reported in SAM, the investigators tested if the proportion of aerotolerant prokaryotes is increased among gut prokaryotes enriched in SAM in comparison to prokaryotes enriched in controls. The investigators further linked this to fecal redox potential and assessed the total number of bacteria in feces by flux cytometry.

Conditions

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Severe Malnutrition Malnutrition Infant Nutrition Disorders Child Nutrition Disorders Kwashiorkor Marasmus

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Senegal

Stool samples on Children with and without severe acute malnutrition in Senegal recruited in 3 locations (Dakar, Dielmo and Ndiop)

Stool samples

Intervention Type BIOLOGICAL

Stool samples

Niger

Stool samples Children with and without severe acute malnutrition in Niger recruited in Niamey

Stool samples

Intervention Type BIOLOGICAL

Stool samples

Interventions

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Stool samples

Stool samples

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Children aged 0-59 months of varying nutritional status from Niamey (Niger) and Dakar (Senegal).
Maximum Eligible Age

59 Months

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Institut Hospitalo-Universitaire Méditerranée Infection

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Matthieu Million

Role: STUDY_CHAIR

Assistance publique des Hôpitaux de Marseille

Locations

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Hopital National

Niamey, , Niger

Site Status

Clinic Notre Dame de L'Esperance, Thiaroye

Dakar, , Senegal

Site Status

Countries

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Niger Senegal

Other Identifiers

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SAMGUT

Identifier Type: -

Identifier Source: org_study_id

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