Study of Intradermal Injections of RCS-01 in Male and Female Subjects

NCT ID: NCT02391935

Last Updated: 2017-10-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

17 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-10-10

Study Completion Date

2017-07-12

Brief Summary

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The primary purpose of this study is to assess the safety profile of RCS-01 injections as compared to placebo injections. This study will also measure the impact these injections will have on skin markers related to aging through evaluation of gene expression profiles.

30 participants will be selected for this study based on their health status, current/past medications, and ability to adhere to protocol-related requirements. At the first visit and after providing informed consent, participants are evaluated against the study inclusion/exclusion criteria and provide blood samples for screening assessments (including virology). If suitable for study participation, participants will provide a biopsy from the scalp from which RCS-01 will be prepared and will have four treatment evaluation sites identified on their buttocks, two on each side.

Study participants will be randomized to one of two treatment subgroups. Participants in the RCS-01 Subgroup (n=24) will receive injections of RCS-01 or placebo or a 'sham' injection (a needle penetration without injection of liquid). Participants in the the Placebo Subgroup (n= 6) will be randomized to receive only injections of placebo or sham injections.

Baseline evaluations of subjects' overall health and skin condition at treatment sites on their buttocks will be performed before receipt of injections at Day 0. In addition to injections delivered at Day 0, the pre-selected treatment evaluation sites will receive intradermal injections of RCS-01 or placebo (cryomedium) or a sham injection four (4) and eight (8) weeks after Day 0 according to a randomization schedule for a total of three (3) injections per treatment site.

All participants will return to the clinic for at least 9 visits during the 52-week follow-up period to monitor long-term safety. At the 12-week time point, 18 randomly-selected participants from the RCS-01 Subgroup will provide biopsies from all injection sites for analysis of skin markers related to aging. At the 26-week time point, the remaining participants will provide biopsies of all injection sites for histopathological analysis.

Detailed Description

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Conditions

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Intrinsic Aging of Skin Skin Wrinkling Solar Degeneration of Skin

Keywords

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Chronic Effect of Ultraviolet Radiation on Normal Skin (Photo-aging)

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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RCS-01

Cultured, autologous hair follicle cells suspended in cryomedium

Group Type EXPERIMENTAL

RCS-01

Intervention Type DRUG

Cultured, autologous hair follicle cells suspended in cryomedium

Placebo

Intervention Type DRUG

Cryomedium

Sham injection

Intervention Type DEVICE

skin penetration of the needle without injection of liquid

Placebo

cryomedium

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DRUG

Cryomedium

Sham injection

Intervention Type DEVICE

skin penetration of the needle without injection of liquid

Interventions

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RCS-01

Cultured, autologous hair follicle cells suspended in cryomedium

Intervention Type DRUG

Placebo

Cryomedium

Intervention Type DRUG

Sham injection

skin penetration of the needle without injection of liquid

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

1. Male or female Caucasian in good health between the age of 50 and 65 years.
2. Female subjects must be post-menopausal for at least one year or surgically sterile.
3. Signed and dated informed consent and willingness to attend all study visits and complete all procedures required by this protocol.
4. The treatment evaluation sites are of uniform skin color without erythema, dark pigmentation or scars that may confound study results.

Exclusion Criteria

1. Any transient skin disorder or infections (e.g. pyoderma, tinea corporis, contact or irritative dermatitis, scleroderma etc.) within 20 cm of the treatment evaluation sites or the presence of tattoos near the treatment sites.
2. Subjects diagnosed with psoriasis, lichen planus, vitiligo, systemic scleroderma, or lupus erythematosus.
3. Any condition that, in the investigator's opinion, would impact subject's safety and/or a subject's ability to complete all study related procedures.
4. History of infection with or positive serology results for human immunodeficiency virus (HIV), hepatitis B or C, or syphilis.
5. Presence/history of hypertrophic scars and/or keloids.
6. Subjects diagnosed with cancer with or without chemotherapy treatment within 3 years prior enrollment.
7. Women who are pregnant or nursing.
8. Current use of any medications not permitted in the study
9. Ongoing or recent participation in a cosmetic and/or clinical research study prior to enrolment.
10. Subject not agreeing to avoid UV exposure (sun, solarium) on the nude buttocks during the study period.
11. Known allergy or hypersensitivity to human serum albumin, DMSO, gentamicin, material of bovine origin and/or local anesthetics.
12. Close affiliation with the investigational site (e.g., a close relative of the investigator or a possibly dependent person (e.g., employee or student of the investigational site)).
13. Subjects diagnosed with coagulopathies or significant thrombocytopenia
Minimum Eligible Age

50 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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JensonR+ Limited

UNKNOWN

Sponsor Role collaborator

PHARMALOG Institut für klinische Forschung GmbH

UNKNOWN

Sponsor Role collaborator

Syreon Corporation

INDUSTRY

Sponsor Role collaborator

Innovacell Biotechnologie AG

INDUSTRY

Sponsor Role collaborator

Datinf GmbH

UNKNOWN

Sponsor Role collaborator

RepliCel Life Sciences, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Alessandra Marini, MD

Role: PRINCIPAL_INVESTIGATOR

IUF Leibniz-Institut für umweltmedizinische Forschung gGmbH

Locations

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IUF Leibniz-Institut für umweltmedizinische Forschung gGmbH

Düsseldorf, , Germany

Site Status

Countries

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Germany

Related Links

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http://www.replicel.com

RepliCel Life Sciences, Inc. Website

Other Identifiers

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RCS-01-001-2014

Identifier Type: -

Identifier Source: org_study_id