Safety and Tolerability of a Bioconjugate Vaccine Against Shigella Flexneri 2a

NCT ID: NCT02388009

Last Updated: 2015-12-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-02-28

Study Completion Date

2015-09-30

Brief Summary

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This is a phase I, single-blind, randomized, placebo-controlled, single-center study in healthy subjects using a staggered approach to dosing.

30 subjects will be randomized to receive 10 μg Flexyn2a candidate vaccine with or without adjuvant or placebo.

Detailed Description

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A total of 30 subjects will be randomly assigned to one of 3 different arms in order to evaluate the safety, tolerability and immunogenicity of a candidate vaccine, formulated with or without adjuvant, and the outcome compared to a placebo control group.

For each active treatment group, 12 subjects will be injected twice with 10 μg Flexyn2a candidate vaccine 4 weeks apart. A control group with 6 subjects will be injected following the same schedule with a placebo solution.

Conditions

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Shigellosis

Keywords

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bioconjugate vaccine safety immunogenicity shigellosis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Participants

Study Groups

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Flexyn2a

2 doses of 10 μg of Flexyn2a will be injected intramuscularly 4 weeks apart

Group Type ACTIVE_COMPARATOR

Flexyn2a

Intervention Type BIOLOGICAL

Intramuscular doses of 0.5 mL

Flexyn2a plus adjuvant

2 doses of 10 μg of Flexyn2a plus adjuvant will be injected intramuscularly 4 weeks apart

Group Type ACTIVE_COMPARATOR

Flexyn2a plus adjuvant

Intervention Type BIOLOGICAL

Intramuscular doses of 0.5 mL

Placebo

2 doses of saline buffer plus adjuvant will be injected intramuscularly 4 weeks apart

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

Intramuscular doses of 0.5 mL

Interventions

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Flexyn2a

Intramuscular doses of 0.5 mL

Intervention Type BIOLOGICAL

Placebo

Intramuscular doses of 0.5 mL

Intervention Type BIOLOGICAL

Flexyn2a plus adjuvant

Intramuscular doses of 0.5 mL

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. Healthy male or female volunteers, age of 18 to 50 years (inclusive) at the time of enrollment.
2. Signed informed consent form.
3. Completion and review of comprehension test (achieved \>70% accuracy)
4. Available for the required follow-up period and scheduled clinic visits.
5. Women: negative pregnancy test with understanding (through informed consent process) to not become pregnant or or breastfeed during the study or within twelve (12) weeks after the last vaccine dose.

Exclusion Criteria

1. Health condition that, in the opinion of the investigator, may interfere with optimal participation in the study or place the volunteer at increased risk of adverse Events (AEs). Study clinicians, in consultation with the PI, will use clinical judgment on a case by-case basis to assess safety risks under this criterion. The PI will consult with the Research Monitor as appropriate.
2. Clinically significant abnormalities on physical examination.
3. Clinically significant abnormalities on basic laboratory screening.
4. Presence of significant unexplained laboratory abnormalities that, in the opinion of the PI, may potentially confound the analysis of the study results
5. Regular use of constipation, antacid or anti-diarrheal medications or treatments.
6. Abnormal stool pattern (fewer than 3 stools per week or more than 3 per day) or loose/liquid stools more than occasionally.
7. Use of immunosuppressive drugs such as corticosteroids or chemotherapeutics that may influence antibody development.
8. Women currently nursing.
9. Participation in research involving another investigational product (defined as receipt of investigational product or exposure to invasive investigational device) within 30 days of planned date of first vaccination or anytime throughout the duration of the study.
10. Positive blood test for HBsAg, hepatitis C Virus (HCV), HIV-1.
11. Positive blood test for HLA-B27.
12. Immunosuppressive illness or immunoglobulin deficiency (serum immunoglobulin A level \< 7 mg/dL or limit of detection of assay).
13. Family history of congenital or hereditary immunodeficiency.
14. Treatment with immunoglobulins or blood products within 3 months from first candidate vaccine injection.
15. History of microbiologically confirmed Shigella infection.
16. Personal or family history of inflammatory arthritis.
17. Personal or family history of irritable bowel syndrome.
18. Received previous experimental Shigella vaccine or live Shigella challenge.
19. Have had diarrhea while traveling outside the United States or lived for 2 or more months during the past 3 years in a country with potentially higher Shigella infection rates, including Africa, South America, Central America, and Asia (except Japan).
20. Occupation involving handling of Shigella bacteria currently, or in the past 3 years.
21. History of allergy to any vaccine.
22. History of allergy to aluminum hydroxide.
23. Serum immunoglobulin G endpoint titer ≥ 2500 to Shigella Lipopolysaccharide.
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Wellcome Trust

OTHER

Sponsor Role collaborator

Naval Medical Research Center

FED

Sponsor Role collaborator

LimmaTech Biologics AG

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Mark S. Riddle, MD Dr Ph

Role: PRINCIPAL_INVESTIGATOR

Navy Medical Research Center

Locations

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WRAIR Clinical Trial Center (CTC)

Silver Spring, Maryland, United States

Site Status

Countries

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United States

References

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Riddle MS, Kaminski RW, Di Paolo C, Porter CK, Gutierrez RL, Clarkson KA, Weerts HE, Duplessis C, Castellano A, Alaimo C, Paolino K, Gormley R, Gambillara Fonck V. Safety and Immunogenicity of a Candidate Bioconjugate Vaccine against Shigella flexneri 2a Administered to Healthy Adults: a Single-Blind, Randomized Phase I Study. Clin Vaccine Immunol. 2016 Dec 5;23(12):908-917. doi: 10.1128/CVI.00224-16. Print 2016 Dec.

Reference Type DERIVED
PMID: 27581434 (View on PubMed)

Other Identifiers

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SF2A-1

Identifier Type: -

Identifier Source: org_study_id