The Role of Immature Myeloid Cells, Monocytes, Dentritic Cells and Neutrophils in Human Pregnancies as Markers for Mature Pregnancy and Spontaneous Labor
NCT ID: NCT02361853
Last Updated: 2015-02-12
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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UNKNOWN
60 participants
OBSERVATIONAL
2015-03-31
2017-03-31
Brief Summary
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CD11b+Gr1+ cells are a heterogeneous population of bone marrow-derived cells (BMDC) that consist of immature myeloid cells (IMCs), and were first described as myeloid-derived suppressor cells. In healthy individuals, IMCs that are generated in the bone marrow differentiate into mature granulocytes, macrophages, or dendritic cells (DCs). These cells have been shown to play an essential role in mediating immune suppression in animal models of human tumors. As a result of tumor-induced alterations in myelopoiesis, IMCs have been found in peripheral blood, lymphoid organs and the tumor tissue itself. An increased population of IMCs was identified in patients with several tumor types. Accordingly, IMCs detected in the peripheral blood of such patients bearing express the common myeloid marker CD33 but lack markers of mature myeloid cells such as the MHC class II molecule HLADR.
IMCs have been shown to actively promote tumor growth and metastasis by modulating the cytokine environment, and through vascular remodeling by promoting angiogenesis.
It has been demonstrated in our laboratory that IMCs infiltrate placentas of pregnant mice and actively promote angiogenesis. These cells show striking similarity to IMCs that populate malignant tumors. Accordingly, human placentas are also infiltrated by a significant population of IMCs. Immunostaining of human placentas showed that IMCs comprise around 25% ( range 10-40%) of total placental CD45+ bone marrow-derived hematopoietic cells and that this population is located close to blood capillaries. The investigators also demonstrated that immature DCs, cells originally described to regulate the adaptive immune response, also promote angiogenesis in models of choroidal neovascularization, endometriosis and tumors.
Tho objective in this proposed study is to compare the abundance of IMC, DC, monocytes and neutrophils counts in blood samples of normal pregnancies: 1. in women in term active labor. 2. In women not in labor. 3. In pre term labor.
According to our previous findings, the investigators hypothesize that IMC's, monocytes and in active delivery (both term and pre term) will be lower than women without signs for labor. The investigators also hypothesize that DC's and neutrophils will be more abundant in active delivery (in term and pre term) than women without signs for labor.
Experimental plan:
Blood count will be collected from pregnant women who agree to participate in the research:
The samples will be sent for the routine complete blood count in Hillel Yaffe hematologic lab and also will be analyzed for IMC's and DC's population in our lab using fluorescent immunostaining with specific monoclonal antibodies and flow cytometry.
IMC's per total CD45 positive hematopoietic cells from the placental tissue will be analyzed using fluorescent immunostaining with specific monoclonal antibodies and flow cytometry . The specific location of these cells in the placenta will be identified within the placental tissue using Immunohistochemistry (IHC). The expression of pro angiogenic genes will be analyzed by RT PCR and Western blotting.
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Detailed Description
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Conditions
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Study Design
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COHORT
PROSPECTIVE
Study Groups
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Active labor at term
Women in active labor normal pregnancy at term
Exposure: abundance of Immature Myeloid Cells counts in blood samples
Exposure: abundance of Immature Dentritic Cells counts in blood samples
Exposure: abundance of Immature monocytes counts in blood samples
Exposure: abundance of Immature neutrophils counts in blood samples
Non-active labor at term
Women, normal pregnancy at term who come for usual follow up without signs for labor ( contraction / "show" discharge/ rupture of membranes).
Exposure: abundance of Immature Myeloid Cells counts in blood samples
Exposure: abundance of Immature Dentritic Cells counts in blood samples
Exposure: abundance of Immature monocytes counts in blood samples
Exposure: abundance of Immature neutrophils counts in blood samples
Active labor, pre-term
Women in active pre term labor (34- 37w) normal pregnancy.
Exposure: abundance of Immature Myeloid Cells counts in blood samples
Exposure: abundance of Immature Dentritic Cells counts in blood samples
Exposure: abundance of Immature monocytes counts in blood samples
Exposure: abundance of Immature neutrophils counts in blood samples
Interventions
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Exposure: abundance of Immature Myeloid Cells counts in blood samples
Exposure: abundance of Immature Dentritic Cells counts in blood samples
Exposure: abundance of Immature monocytes counts in blood samples
Exposure: abundance of Immature neutrophils counts in blood samples
Eligibility Criteria
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Inclusion Criteria
* Women, normal pregnancy at term who come for usual follow up without signs for labor ( contraction / "show" discharge/ rupture of membranes).
* Women in active pre term labor (34- 37w) normal pregnancy.
Exclusion Criteria
* Signs for infection ( fever \>38)
* Women in pre-term birth under 34 weeks.
* Administration of Celeston in 2 weeks prior to enrollment.
* Any complication to the women or fetus which require induction of labor ( Pre-eclampsia, Gestational Diabetes Mellitus A2, pre gestational diabetes, Intrauterine Growth Restriction).
18 Years
FEMALE
No
Sponsors
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Hillel Yaffe Medical Center
OTHER_GOV
Responsible Party
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Central Contacts
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Other Identifiers
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0086-14-HYMC
Identifier Type: -
Identifier Source: org_study_id
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