A Safety and Immunogenicity Study of Heterologous and Homologous Prime-Boost Ebola Vaccine Regimens in Healthy Participants

NCT ID: NCT02325050

Last Updated: 2017-06-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

164 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-01-08

Study Completion Date

2017-05-08

Brief Summary

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The purpose of this study is to test the safety and immunogenicity of MVA-BN-Filo and Ad26.ZEBOV as heterologous and homologous prime-boost vaccine regimens in healthy adult participants.

Detailed Description

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This study consists of 3 parts: the first and third part with standard doses and the second part with higher doses. All parts are randomized, placebo-controlled, observer-blind to evaluate the safety, tolerability and immunogenicity of MVA-BN-Filo and Ad26.ZEBOV administered in different doses, sequences and schedules to healthy adult participants. The study consists of a screening period of up to 28 days, a vaccination period in which participants will be vaccinated at baseline (Day 1) followed by a boost on Day 15, 29, or 57, and third vaccine 1-year post-prime (3rd vaccination is optional for subjects in groups 1-8). The total duration of the study will be about 1 year for participants who wiil receive boost vaccine and about 3 months for participants who will receive placebo and 2 year for participants who will receive a 3rd dose. Safety will be monitored during the study.

Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Group 1

Participants will receive MVA-BN-Filo/ Ad26.ZEBOV (Day 1 /Day 15) or Placebo (Day 1/Day 15). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 2

Participants will receive MVA-BN-Filo/Ad26.ZEBOV (Day 1 /Day 29) or placebo (Day 1/Day 29). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 3

Participants will receive MVA-BN-Filo /Ad26.ZEBOV/ (Day 1/Day 57) or placebo (Day 1/Day 57). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 4

Participants will receive Ad26.ZEBOV/ MVA-BN-Filo (Day 1/Day 29) or placebo (Day 1/Day 29). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 5

Participants will receive MVA-BN-Filo (Day 1 and Day 15) or placebo (Day 1 and Day 15). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 6

Participants will receive Ad26.ZEBOV (Day 1 and Day 15) or placebo (Day 1 and Day 15). Participants will receive MVA-BN-Filo (1\*10\^8 TCID50) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 7

Participants will receive Ad26.ZEBOV/ MVA-BN-Filo (Day 1/Day 15) or Placebo (Day 1/Day 15). Participants will receive Ad26.ZEBOV (5x10\^10 vp) on Day 360.

Group Type EXPERIMENTAL

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 4.4\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 8

Participants will receive Ad26.ZEBOV/ MVA-BN-Filo (Day 1 /Day 29) or Placebo (Day 1/Day 29). Participants will receive Ad26.ZEBOV (1x10\^11 vp) on Day 360.

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 4.4\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 1\*10\^11 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 9

Participants will receive MVA-BN-Filo/ Ad26.ZEBOV (Day 1 /Day 8) or Placebo (Day 1/Day 8).

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Group 10

Participants will receive MVA-BN-Filo/ Ad26.ZEBOV (Day 1 /Day 15) or Placebo (Day 1/Day 15).

Group Type EXPERIMENTAL

MVA-BN-Filo

Intervention Type BIOLOGICAL

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Ad26. ZEBOV

Intervention Type BIOLOGICAL

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Placebo

Intervention Type OTHER

One 0.5 mL IM injection of 0.9% saline.

Interventions

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MVA-BN-Filo

One 0.5 milliliter (ml) intramuscular (IM) injection of 1\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Intervention Type BIOLOGICAL

Ad26. ZEBOV

One 0.5 mL IM injection of 5\*10\^10 viral particles (vp).

Intervention Type BIOLOGICAL

MVA-BN-Filo

One 0.5 milliliter (ml) intramuscular (IM) injection of 4.4\*10\^8, (50%Tissue Culture Infectious Dose \[TCID50\]).

Intervention Type BIOLOGICAL

Ad26. ZEBOV

One 0.5 mL IM injection of 1\*10\^11 viral particles (vp).

Intervention Type BIOLOGICAL

Placebo

One 0.5 mL IM injection of 0.9% saline.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Must be healthy on the basis of physical examination, medical history, and the investigator's clinical judgment
* Have a body mass index (BMI) ≥18.5 and \<35.0 kg/m2
* Women of childbearing potential must have a negative serum β-human chorionic gonadotropin pregnancy test at screening, a negative urine pregnancy test immediately prior to each study vaccine administration, and practice adequate birth control measures from 28 days before the prime vaccination until at least 3 months after the boost vaccination as specified in the study protocol. If not heterosexually active at screening, must agree to practice adequate birth control measures if they become heterosexually active during their participation in the study (from screening onwards until at least 3 months after the boost vaccination). Agree not to donate eggs (ova, oocytes) for the purposes of assisted reproduction during participation in the study (from screening onwards until at least 3 months after the boost vaccination)
* Women of non-childbearing potential, defined as postmenopausal (\>45 years of age with amenorrhea for ≥2 years or any age with amenorrhea for ≥6 months and serum follicle-stimulating hormone \[FSH\] \>40 mIU/mL) or surgically sterile (hysterectomy, bilateral tubal ligation, or bilateral oophorectomy), are not required to use the birth control methods as specified in the study protocol
* A man who has not had a vasectomy and is sexually active with a woman of childbearing potential must agree to use a double-barrier method of birth control, such as either condom with spermicidal foam/gel/film/cream/suppository or partner with occlusive cap (diaphragm or cervical/vault caps) with spermicidal foam/gel/film/cream/suppository. In case the female partner is using an adequate method of birth control, a single-barrier method of birth control for the male subject is acceptable. Men must also agree not to donate sperm during their participation in the study (from screening onwards until at least 3 months after the boost vaccination)
* Must be available and willing to participate for the duration of the study visits and follow-up, provide verifiable identification, and have a means to be contacted

Exclusion Criteria

* Has been vaccinated with a candidate Ebola vaccine
* Has been diagnosed with Ebola disease or exposed to Ebola including travel to West Africa in the last 12 months. West Africa includes but is not limited to the countries of Guinea, Liberia, Mali, and Sierra Leone. Participants who anticipate traveling to epidemic Ebola areas before the start of the long-term follow-up period will also be excluded from enrollment into the study
* Has received any Ad26- or MVA-based candidate vaccine in the past
* Known allergy or history of anaphylaxis or other serious adverse reactions to vaccines or vaccine products (including any of the constituents of the study vaccines), including known allergy to egg or aminoglycosides
* A woman who is pregnant or breast-feeding, or planning to become pregnant while enrolled in the study or within 3 months after the boost vaccination
* History of diabetes mellitus type 1 or type 2, including cases controlled with diet alone; thyroidectomy, or thyroid disease requiring medication during the last 12 months; uncontrolled hypertension as defined in the study protocol; or, major psychiatric illness and/or substance abuse problems during the past 12 months that in the opinion of the investigator would preclude participation
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Janssen Vaccines & Prevention B.V.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Janssen Vaccines & Prevention B.V. Clinical Trial

Role: STUDY_DIRECTOR

Janssen Vaccines & Prevention B.V.

Locations

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Rockville, Maryland, United States

Site Status

Countries

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United States

Other Identifiers

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VAC52150EBL1002

Identifier Type: OTHER

Identifier Source: secondary_id

CR106479

Identifier Type: -

Identifier Source: org_study_id

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