Trial Outcomes & Findings for Dietary Flavanols and Dentate Gyrus Function (NCT NCT02312310)

NCT ID: NCT02312310

Last Updated: 2020-06-11

Results Overview

The ModBent task is divided into two parts. During the matching trials (top), participants were shown a complex stimulus for 10 s. Following a 1-s inter-trial interval, they were required to select, via a key press, which of two stimuli matched the one they had just studied, as quickly and as accurately as possible. Following 41 matching trials, participants were shown a series of 82 stimuli (bottom), 41 of which appeared on the initial study set during the matching trials and 41 of which were foils. They were required to indicate, as quickly as possible, whether each stimulus appeared earlier (yes response) or was new (no response). The primary variable of the ModBent task is the mean RT for correct rejections of foils during the recognition trials

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

211 participants

Primary outcome timeframe

baseline and 12 weeks

Results posted on

2020-06-11

Participant Flow

first consent; 1/13/2016 Final data collection session: 11/21/2018 All data collected at Columbia University Medical Center

1. consent: exclusions: 5 BMI too high; 66 MOCA too low; 4 elevated blood pressure; 16 other; 17 lost to follow-up 2. two week run-in period: exclusions: 24 failed, 17 withdrew or lost to follow-up 3. baseline assessments: exclusions: 1 lost to follow-up

Participant milestones

Participant milestones
Measure
0 mg Cocoa Flavanol
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Overall Study
STARTED
53
53
53
52
Overall Study
COMPLETED
51
51
50
52
Overall Study
NOT COMPLETED
2
2
3
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Dietary Flavanols and Dentate Gyrus Function

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
0 mg
n=53 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg
n=53 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg
n=53 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg
n=52 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Total
n=211 Participants
Total of all reporting groups
Age, Continuous
61.47 years
STANDARD_DEVIATION 6.78 • n=5 Participants
60.81 years
STANDARD_DEVIATION 6.71 • n=7 Participants
61 years
STANDARD_DEVIATION 6.15 • n=5 Participants
61.23 years
STANDARD_DEVIATION 6.37 • n=4 Participants
61.13 years
STANDARD_DEVIATION 6.47 • n=21 Participants
Sex: Female, Male
Female
31 Participants
n=5 Participants
30 Participants
n=7 Participants
30 Participants
n=5 Participants
29 Participants
n=4 Participants
120 Participants
n=21 Participants
Sex: Female, Male
Male
22 Participants
n=5 Participants
23 Participants
n=7 Participants
23 Participants
n=5 Participants
23 Participants
n=4 Participants
91 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
4 Participants
n=5 Participants
4 Participants
n=7 Participants
8 Participants
n=5 Participants
4 Participants
n=4 Participants
20 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
49 Participants
n=5 Participants
49 Participants
n=7 Participants
45 Participants
n=5 Participants
48 Participants
n=4 Participants
191 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Race (NIH/OMB)
Asian
4 Participants
n=5 Participants
2 Participants
n=7 Participants
4 Participants
n=5 Participants
3 Participants
n=4 Participants
13 Participants
n=21 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
1 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
1 Participants
n=21 Participants
Race (NIH/OMB)
Black or African American
7 Participants
n=5 Participants
7 Participants
n=7 Participants
8 Participants
n=5 Participants
8 Participants
n=4 Participants
30 Participants
n=21 Participants
Race (NIH/OMB)
White
41 Participants
n=5 Participants
40 Participants
n=7 Participants
38 Participants
n=5 Participants
41 Participants
n=4 Participants
160 Participants
n=21 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=5 Participants
3 Participants
n=7 Participants
3 Participants
n=5 Participants
0 Participants
n=4 Participants
7 Participants
n=21 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Region of Enrollment
United States
53 Participants
n=5 Participants
53 Participants
n=7 Participants
53 Participants
n=5 Participants
52 Participants
n=4 Participants
211 Participants
n=21 Participants
alternative Healthy Eating Index (aHEI)
65.09 units on a scale
STANDARD_DEVIATION 11.44 • n=5 Participants
68.78 units on a scale
STANDARD_DEVIATION 10.82 • n=7 Participants
64.83 units on a scale
STANDARD_DEVIATION 14.3 • n=5 Participants
66.74 units on a scale
STANDARD_DEVIATION 10.84 • n=4 Participants
66.36 units on a scale
STANDARD_DEVIATION 12 • n=21 Participants
5-(3',4'-dihydroxyphenyl)-γ-valerolactone (gVLM)
19.7 nM
STANDARD_DEVIATION 24.23 • n=5 Participants
20.98 nM
STANDARD_DEVIATION 34.28 • n=7 Participants
44.32 nM
STANDARD_DEVIATION 72.4 • n=5 Participants
36.59 nM
STANDARD_DEVIATION 72.06 • n=4 Participants
30.44 nM
STANDARD_DEVIATION 55.84 • n=21 Participants

PRIMARY outcome

Timeframe: baseline and 12 weeks

The ModBent task is divided into two parts. During the matching trials (top), participants were shown a complex stimulus for 10 s. Following a 1-s inter-trial interval, they were required to select, via a key press, which of two stimuli matched the one they had just studied, as quickly and as accurately as possible. Following 41 matching trials, participants were shown a series of 82 stimuli (bottom), 41 of which appeared on the initial study set during the matching trials and 41 of which were foils. They were required to indicate, as quickly as possible, whether each stimulus appeared earlier (yes response) or was new (no response). The primary variable of the ModBent task is the mean RT for correct rejections of foils during the recognition trials

Outcome measures

Outcome measures
Measure
0 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
n=50 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
n=52 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Modified Benton Recognition Task (ModBent)
Baseline
2807.98 ms
Standard Deviation 1733.36
2579.94 ms
Standard Deviation 1026.18
2603.65 ms
Standard Deviation 999.31
2732.79 ms
Standard Deviation 1367.96
Modified Benton Recognition Task (ModBent)
12 Weeks
2671.50 ms
Standard Deviation 1431.17
2489.84 ms
Standard Deviation 1135.40
2381.68 ms
Standard Deviation 929.14
2653.01 ms
Standard Deviation 1289.18

SECONDARY outcome

Timeframe: baseline and 12 weeks

measured in functional MRI. we generated high-resolution CBV brain maps using gadolinium-enhanced T1-weighted scans acquired perpendicular to the hippocampal long-axis, with sub-millimeter in-plane resolution of 0.68 × 0.68 mm and slice thickness of 3 mm. To perform group-wise voxel-based analyses on the whole hippocampal circuit, we first isolated hippocampal subfields using FreeSurfer segmentations and thresholded posterior probabilities to generate a composite mask of the bilateral hippocampal formation. These masked T1-weighted images were then used to generate a group-wise template to which individual images were co-registered using a diffeomorphic technique that maximizes cross-correlation among images.

Outcome measures

Outcome measures
Measure
0 mg Cocoa Flavanol
n=14 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
n=10 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
n=13 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
n=12 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Dentate Gyrus Cerebral Blood Volume
Baseline
2.44 % cerebral blood volume
Standard Deviation .81
2.17 % cerebral blood volume
Standard Deviation .69
2.11 % cerebral blood volume
Standard Deviation 0.47
1.85 % cerebral blood volume
Standard Deviation 0.67
Dentate Gyrus Cerebral Blood Volume
12 Weeks
2.25 % cerebral blood volume
Standard Deviation .57
2.11 % cerebral blood volume
Standard Deviation .52
2.01 % cerebral blood volume
Standard Deviation .71
2.23 % cerebral blood volume
Standard Deviation .59

SECONDARY outcome

Timeframe: 20 weeks

The ModBent task is divided into two parts. During the matching trials (top), participants were shown a complex stimulus for 10 s. Following a 1-s inter-trial interval, they were required to select, via a key press, which of two stimuli matched the one they had just studied, as quickly and as accurately as possible. Following 41 matching trials, participants were shown a series of 82 stimuli (bottom), 41 of which appeared on the initial study set during the matching trials and 41 of which were foils. They were required to indicate, as quickly as possible, whether each stimulus appeared earlier (yes response) or was new (no response). The primary variable of the ModBent task is the mean RT for correct rejections of foils during the recognition trials

Outcome measures

Outcome measures
Measure
0 mg Cocoa Flavanol
n=50 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
n=49 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
n=50 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Modified Benton Recognition Task (ModBent)
2542.76 ms
Standard Deviation 1278.87
2475.35 ms
Standard Deviation 1512.88
2373.78 ms
Standard Deviation 934.84
2550.18 ms
Standard Deviation 1165.92

SECONDARY outcome

Timeframe: Baseline and 12 Weeks

In learning Trials 1 to 3 the participant is read 20 unique, semantically/phonemically unrelated words (List A) and is to free recall those words after each trial. Next the participant is read a list of 20 unique words (List B) and is to recall as many as possible. This trial is followed by a short delay free recall trial: the participant is to recall as many words as possible from List A. After a 1.5-hour, the participant is asked to long delay free recall words from List A and then from List B. This is followed by a 66-item forced recognition trial: the participant is read a list of 66 words and asked to distinguish List A words from semantic and phonemic distractors, including words from List B. Finally, a source memory trial is administered: the examiner serially reads words from List A and B. The participant is to specify from which list each word came.Higher score indicates better performance. score ranges from 0 to 60.

Outcome measures

Outcome measures
Measure
0 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
n=50 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
n=52 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
Modified Rey Auditory Verbal Learning Test
Baseline
38.91 units on a scale
Standard Deviation 8.45
37.25 units on a scale
Standard Deviation 8.19
39.75 units on a scale
Standard Deviation 7.38
39.96 units on a scale
Standard Deviation 9.62
Modified Rey Auditory Verbal Learning Test
12 Weeks
39.49 units on a scale
Standard Deviation 9.70
38.69 units on a scale
Standard Deviation 9.30
41.10 units on a scale
Standard Deviation 8.50
41.92 units on a scale
Standard Deviation 8.48

SECONDARY outcome

Timeframe: Baseline and 12 Weeks

Participants are presented with a series of stimuli, each of which is both visually and auditorily presented by computer. A picture of each stimulus is displayed on the computer monitor for 2 seconds while the name of the stimulus is simultaneously being read via a computerized voice; stimuli are presented one after another. The participant must remember each stimulus in a series, mentally reorder them from smallest to largest, and recite the names of the stimuli in this order. List Sorting scores are based upon Total Score. Higher score indicated better performance.Score ranges from 0-100.

Outcome measures

Outcome measures
Measure
0 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 0 mg cocoa flavanol for 12 weeks
260 mg Cocoa Flavanol
n=51 Participants
daily consumption of capsules containing 260 mg cocoa flavanol for 12 weeks;
510 mg Cocoa Flavanol
n=50 Participants
daily consumption of capsules containing 510 mg cocoa flavanol for 12 weeks;
770 mg Cocoa Flavanol
n=52 Participants
daily consumption of capsules containing 770 mg cocoa flavanol for 12 weeks;
NIH Toolbox Cognition Battery (NIHTB-CB): The List Sorting Test
Baseline
52.89 units on a scale
Standard Deviation 8.85
56.81 units on a scale
Standard Deviation 8.91
54.60 units on a scale
Standard Deviation 9.15
55.18 units on a scale
Standard Deviation 8.65
NIH Toolbox Cognition Battery (NIHTB-CB): The List Sorting Test
12 Weeks
53.34 units on a scale
Standard Deviation 7.44
56.10 units on a scale
Standard Deviation 8.47
54.86 units on a scale
Standard Deviation 8.56
53.46 units on a scale
Standard Deviation 9.23

Adverse Events

0 mg Cocoa Flavanol

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

260 mg Cocoa Flavanol

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

510 mg Cocoa Flavanol

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

770 mg Cocoa Flavanol

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Scott A. Small, MD

Columbia University Medical Center

Phone: 212-305-1269

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place