Variation in Serum Prolactin Levels During IntraCytoplasmic Sperm Injection

NCT ID: NCT02292953

Last Updated: 2014-11-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

90 participants

Study Classification

OBSERVATIONAL

Study Start Date

2014-11-30

Study Completion Date

2015-06-30

Brief Summary

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The purpose of the study is to determine whether or not stressful conditions such as ovum pick and awaiting Embryo Transfer is associated with significant increase in serum Prolactin (PRL) concentration in some normo-prolactinemic patients undergoing intracytoplasmatic sperm injection (ICSI) \& possible effect on implantation \& pregnancy rate.

Detailed Description

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Methodology in details:

After applying inclusion and exclusion criteria to infertile patients scheduled for start of ICSI cycle at kasr alainy assisted reproduction unit, and taking the patient's informed consent for all blood samples that will be drawn from her.

The baseline serum prolactin level is recorded and the first blood sample is scheduled to be taken in operating room just before induction of anesthesia for ovum pickup. A Second blood sample will be taken two hours after aspiration of the last follicle in recovery room post-operatively.

A third final blood sample will be taken before Embryo transfer .The blood samples will be sent immediately to central laboratory in Kasr Alainy Cairo University Hospital after being obtained on every occasion, and Serum prolactin will be analyzed by immunoassay in each time.

Descriptive data such as age, type of infertility \& cause will be recorded. Also result of every serum prolactin level will be recorded as well as type of protocol used for ovulation induction, number of oocytes retrieved at ovum pick up, embryo grading and day of transfer.

Serum beta human chorionic gonadotropin (B-hCG) will be drawn from the patient 2 weeks after the date of embryo transfer to determine pregnancy and recorded.

Statistical analysis will be done for the laboratory results, All statistical calculations were performed using the computer program SPSS (Statistical Package for the Social Science; SPSS Inc.) version 15 for Microsoft Windows

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Conditions

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Infertility

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

PROSPECTIVE

Interventions

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Serum Prolactin levels

blood samples will be drawn from patients undergoing ICSI to analyse serum prolactin levels

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Age: adult female \>20 years old.
* Complaining of infertility 1ry or 2ry \& scheduled for ICSI.

Exclusion Criteria

* Hyperprolactinemia patients.
* Severe Male factor infertility
* Frozen embryo transfer cycles
* Uterine Anomalies.
* Repeated implantation failure in ICSI.
* Any contraindications for Pregnancy as(serious uncontrolled medical diseases such as Diabetes, heart disease, hypertension), mental illnesses, ovarian tumors,cancer of any location and Acute inflammatory disease).
* On medication that is known to alter prolactin levels e.g antipsychotics, Atypical agents and risperidone
* Thyroid dysfunction.
* Medical disorders affecting serum prolactin eg acromegaly ,chronic renal failure and hypothyroidism.
Minimum Eligible Age

20 Years

Maximum Eligible Age

43 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Kasr El Aini Hospital

OTHER

Sponsor Role lead

Responsible Party

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Ahmed M.Kamel

Lecturer of Obstetric & Gynecology

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Ahmed M Kamel, MD

Role: PRINCIPAL_INVESTIGATOR

Lecturer Of obstetrics & Gynecology

Locations

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11562

Ḩadā’iq al Qubbah, Cairo Governorate, Egypt

Site Status RECRUITING

Countries

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Egypt

Central Contacts

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Ahmed M Kamel, MD

Role: CONTACT

01120022332

Facility Contacts

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Ahmed M KameL, M.D

Role: primary

01120022332

References

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Boyers SP, Lavy G, Russell JB, Polan ML, DeCherney AH. Serum prolactin response to embryo transfer during human in vitro fertilization and embryo transfer. J In Vitro Fert Embryo Transf. 1987 Oct;4(5):269-72. doi: 10.1007/BF01555202.

Reference Type BACKGROUND
PMID: 2961832 (View on PubMed)

Kamel A, Halim AA, Shehata M, AlFarra S, El-Faissal Y, Ramadan W, Hussein AM. Changes in serum prolactin level during intracytoplasmic sperm injection, and effect on clinical pregnancy rate: a prospective observational study. BMC Pregnancy Childbirth. 2018 May 9;18(1):141. doi: 10.1186/s12884-018-1783-4.

Reference Type DERIVED
PMID: 29739353 (View on PubMed)

Other Identifiers

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A13952014

Identifier Type: -

Identifier Source: org_study_id