SEGATROM: Sensory and Gastrointestinal Impact of Taste Receptor Variants on Human Metabolism and Nutrition

NCT ID: NCT02219295

Last Updated: 2020-06-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

97 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-12-31

Study Completion Date

2014-09-30

Brief Summary

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We investigate the impact of taste receptors (sweet, bitter and umami) on human metabolism. There ist growing evidence, that components of the taste signalling system, including the taste receptor proteins , have important functions in the regulation of metabolic parameters in non-gustatory tissues.

Detailed Description

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We apply different sweet, bitter and umami taste compounds in a single dose in the human intervention. We use substances as for instance glucose, sucrose,saccharin and lactisol in the sweet part, and substances from cabbage, beer or red endive in the bitter part. For umami we take glutamate.

Blood samples are collected at baseline and 6 times thereafter up to 3hours, to analyse the incretins GLP-1(glucagon-like peptide 1) and GIP(gastrointestinal polypeptide) and other peptide hormones as insulin and PYY(peptide tyrosin trosin) in human beings.

Gastrointestinal mucosa is taken by gastroscopy from the lower duodenum from study subjects for analysis for the presence or absence of functional taste receptors in gastrointestinal tissues.

Conditions

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Disorder of Glucose Regulation

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

FACTORIAL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants

Study Groups

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sweet -block

application of different sugars, artificial sweeteners and sweet-taste antagonists in a single dose in 300 ml tap water every week up to 7 times and collection of blood samples over 3 hours

Group Type EXPERIMENTAL

sweet block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin

bitter block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose

umami-block

Intervention Type DIETARY_SUPPLEMENT

oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

bitter block

application of purified bitter tasting ingredients of vegetables and hop in 300 ml tap water , collection of blood samples for 3h (-15,0,15,30,60,120,180 min)

Group Type EXPERIMENTAL

sweet block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin

bitter block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose

umami-block

Intervention Type DIETARY_SUPPLEMENT

oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

umami-block

application of glutamate and glutamate +IMP

same procedure as above

Group Type EXPERIMENTAL

sweet block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin

bitter block

Intervention Type DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose

umami-block

Intervention Type DIETARY_SUPPLEMENT

oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

gastroscopy

gastroscopy with and without oral intervention with artificial sweetener saccharin to take tissue samples from the upper bowel for the investigation of taste receptor cells in the upper bowel in human beings

Group Type OTHER

gastroscopy

Intervention Type OTHER

gastroscopy with and without oral application of saccharin, tissue samples from th upper duodenum by endoscopy for the detection of taste receptor cells in the upper bowel

Interventions

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sweet block

oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin

Intervention Type DIETARY_SUPPLEMENT

bitter block

oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose

Intervention Type DIETARY_SUPPLEMENT

umami-block

oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

Intervention Type DIETARY_SUPPLEMENT

gastroscopy

gastroscopy with and without oral application of saccharin, tissue samples from th upper duodenum by endoscopy for the detection of taste receptor cells in the upper bowel

Intervention Type OTHER

Other Intervention Names

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Sugars as sucrose, glucose, palatinose, galactose artificial sweeteners as saccharin sweet taste antagonist as lactisol application of sinigrin lactucopicrin iso-alpha-acid application of glutamate and glutamate+IMP, tissue samples sweet taste receptor stimualtion artificial sweetener saccharin SGLT-1

Eligibility Criteria

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Inclusion Criteria

* Healthy persons

Exclusion Criteria

* Tumor in history
* Pregnancy
* Severe illness in history
* Bodymass index below 18
Minimum Eligible Age

18 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Technical University of Munich

OTHER

Sponsor Role collaborator

German Institute of Human Nutrition

OTHER

Sponsor Role lead

Responsible Party

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Prof. Dr. med. Andreas F. H. Pfeiffer

Prof. Dr. med.

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Andreas FH Peiffer, Prof

Role: PRINCIPAL_INVESTIGATOR

German Institute of Human Nutrition

Locations

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Department of Clinical Nutrition

Bergholz-Rehbrücke, Brandenburg, Germany

Site Status

Countries

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Germany

References

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Zhang J, Schafer SM, Kabisch S, Csanalosi M, Schuppelius B, Kemper M, Markova M, Meyer NMT, Pivovarova-Ramich O, Keyhani-Nejad F, Rohn S, Pfeiffer AFH. Implication of sugar, protein and incretins in excessive glucagon secretion in type 2 diabetes after mixed meals. Clin Nutr. 2023 Apr;42(4):467-476. doi: 10.1016/j.clnu.2023.02.011. Epub 2023 Feb 21.

Reference Type DERIVED
PMID: 36857956 (View on PubMed)

Keyhani-Nejad F, Kemper M, Schueler R, Pivovarova O, Rudovich N, Pfeiffer AF. Effects of Palatinose and Sucrose Intake on Glucose Metabolism and Incretin Secretion in Subjects With Type 2 Diabetes. Diabetes Care. 2016 Mar;39(3):e38-9. doi: 10.2337/dc15-1891. Epub 2015 Dec 30. No abstract available.

Reference Type DERIVED
PMID: 26721819 (View on PubMed)

Other Identifiers

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BMBF 11005D

Identifier Type: -

Identifier Source: org_study_id

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