Trial Outcomes & Findings for Effect of Oral Caffeine and L-Citrulline Supplementation on Arterial Function in Healthy Males (NCT NCT02214290)

NCT ID: NCT02214290

Last Updated: 2020-12-29

Results Overview

Aortic blood pressure at rest and 30 minutes after acute ingestion of 200 mg of caffeine before and after 7 days of either 6g/day of Citrulline or 6 g/day of Placebo.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

16 participants

Primary outcome timeframe

Baseline and Day 7 (Calculated change between rest and 30 minutes post-caffeine ingestion at these time points)

Results posted on

2020-12-29

Participant Flow

Sixteen young healthy men participated in this study. All subjects were non-smokers, recreationally active, and free of apparent cardiovascular-metabolic diseases based on medical history.

Of the 42 individuals screened, 16 individuals completed the intervention in its entirety.

Participant milestones

Participant milestones
Measure
Citrulline First, Then Placebo
Each participant received L-Citrulline (6g/day) for a 7 day period. This was followed by a 14 day washout period. Finally, the same participants then received a Placebo (6g/day maltodextrin) for a 7 day period.
Placebo First, Then L-Citrulline
Each participant received a Placebo (6g/day maltodextrin) for a 7 day period. This was followed by a 14 day washout period. Finally, the same participants then received a L-Citrulline (6g/day) for a 7 day period.
First Intervention (7 Days)
STARTED
8
8
First Intervention (7 Days)
COMPLETED
8
8
First Intervention (7 Days)
NOT COMPLETED
0
0
Washout (14 Days)
STARTED
8
8
Washout (14 Days)
COMPLETED
8
8
Washout (14 Days)
NOT COMPLETED
0
0
Second Intervention (7 Days)
STARTED
8
8
Second Intervention (7 Days)
COMPLETED
8
8
Second Intervention (7 Days)
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
L-Citrulline or Placebo
n=16 Participants
Placebo and L-Citrulline pills produced by NOW FOODS, USA Citrulline or Placebo (both 6g/day) were given for a 7 day supplementation period. This was followed by a 14 day washout period and crossover to the other supplement (L-Citrulline or Placebo) for 7 days. Measurements of arterial function were performed to determine if the supplements had any significant effect on blood pressure control/pressure wave reflection at rest and immediately after ingestion of 200 milligrams of caffeine.
Age, Categorical
<=18 years
0 Participants
n=16 Participants
Age, Categorical
Between 18 and 65 years
16 Participants
n=16 Participants
Age, Categorical
>=65 years
0 Participants
n=16 Participants
Age, Continuous
23 years
STANDARD_DEVIATION 3 • n=16 Participants
Sex: Female, Male
Female
0 Participants
n=16 Participants
Sex: Female, Male
Male
16 Participants
n=16 Participants

PRIMARY outcome

Timeframe: Baseline and Day 7 (Calculated change between rest and 30 minutes post-caffeine ingestion at these time points)

Population: Young, apparently healthy individuals, aged 18-40 years old.

Aortic blood pressure at rest and 30 minutes after acute ingestion of 200 mg of caffeine before and after 7 days of either 6g/day of Citrulline or 6 g/day of Placebo.

Outcome measures

Outcome measures
Measure
Citrulline
n=16 Participants
Participants received 6g/day of Citrulline for 7 days
Placebo
n=16 Participants
Participants received 6 g/day of Maltodextrin for 7 days.
Aortic Blood Pressure
Baseline
5 mmHg
Standard Deviation 1
6 mmHg
Standard Deviation 1
Aortic Blood Pressure
7 Days
4 mmHg
Standard Deviation 1
4 mmHg
Standard Deviation 1

SECONDARY outcome

Timeframe: Baseline and Day 7 (Calculated change between rest and 30 minutes post-caffeine ingestion at these time points)

Population: Young, apparently healthy adults, aged 18-40 years.

Reflected wave pressure at rest and 30 minutes after ingestion of 200mg of caffeine after 7 days supplementation of 6g/day of either L-Citrulline or Placebo.

Outcome measures

Outcome measures
Measure
Citrulline
n=16 Participants
Participants received 6g/day of Citrulline for 7 days
Placebo
n=16 Participants
Participants received 6 g/day of Maltodextrin for 7 days.
Changes in Reflected Wave Pressure (P2)
Baseline
5 mmHg
Standard Deviation 1
5 mmHg
Standard Deviation 1
Changes in Reflected Wave Pressure (P2)
7 Days
4 mmHg
Standard Deviation 1
5 mmHg
Standard Deviation 1

SECONDARY outcome

Timeframe: Baseline and Day 7 (Calculated change between rest and 30 minutes post-caffeine ingestion at these time points)

Population: young, apparently healthy adults, aged 18-40 years

Femoral-ankle pulse wave velocity at rest and 30 minutes after ingestion of 200mg of caffeine after 7 days supplementation of 6g/day of either L-Citrulline or Placebo.

Outcome measures

Outcome measures
Measure
Citrulline
n=16 Participants
Participants received 6g/day of Citrulline for 7 days
Placebo
n=16 Participants
Participants received 6 g/day of Maltodextrin for 7 days.
Changes in Femoral-ankle Pulse Wave Velocity After 7 Days of Either L-Citrulline or Placebo (6g/Day)
Baseline
0.6 m/s
Standard Deviation 0.4
0.7 m/s
Standard Deviation 0.5
Changes in Femoral-ankle Pulse Wave Velocity After 7 Days of Either L-Citrulline or Placebo (6g/Day)
7 Days
0.4 m/s
Standard Deviation 0.4
0.6 m/s
Standard Deviation 0.4

Adverse Events

Citrulline

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Arturo Figueroa

Texas Tech University

Phone: 8068345587

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place