Trial Outcomes & Findings for Physiologic Adaptations to Interval and Continuous Running at Low Volume and Vigorous Intensity Over 14-Weeks. (NCT NCT01816451)

NCT ID: NCT01816451

Last Updated: 2014-04-29

Results Overview

The relative maximal oxygen uptake was taken by individual connected to a metabolic gas analyzer (VO-2000, Aerosport, Medgraphics, St. Paul, Minnesota) through which the gas samples were collected and measured each 10 seconds during the test. The participants were submitted to a ramp protocol with an initial velocity of 8.0 km/h (0% of inclination), progressive increments, a final velocity of 18 km/h (2% de inclination). The duration was equal to 10 minutes or until voluntary exhaustion.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

35 participants

Primary outcome timeframe

Pre and post 14 weeks

Results posted on

2014-04-29

Participant Flow

Participants were randomly assigned on August 2012 to either an interval,continuous training or control group from Sept to Dec. Tests and training were performed in a controlled laboratory condition.Tests were each separated by 24-48 h.One week before baseline they did a familiarization to submaximal test and all physiological measures.

Due to factors outside the present study, three individuals were removed from the program. Two individuals during maximal exercise test (VO2) showed clinical impairment test being interrupted by the doctor and the third individual presented a plantar fasciitis in the early familiarization.

Participant milestones

Participant milestones
Measure
Interval
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
The control group did not do the running training program. Control did their normal physical activities.
Overall Study
STARTED
13
11
8
Overall Study
COMPLETED
13
11
8
Overall Study
NOT COMPLETED
0
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Physiologic Adaptations to Interval and Continuous Running at Low Volume and Vigorous Intensity Over 14-Weeks.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Total
n=32 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Age, Categorical
Between 18 and 65 years
13 Participants
n=5 Participants
11 Participants
n=7 Participants
8 Participants
n=5 Participants
32 Participants
n=4 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Age, Continuous
30.2 years
STANDARD_DEVIATION 0.8 • n=5 Participants
30.5 years
STANDARD_DEVIATION 1.0 • n=7 Participants
29.1 years
STANDARD_DEVIATION 4.4 • n=5 Participants
30 years
STANDARD_DEVIATION 2 • n=4 Participants
Sex: Female, Male
Female
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Sex: Female, Male
Male
13 Participants
n=5 Participants
11 Participants
n=7 Participants
8 Participants
n=5 Participants
32 Participants
n=4 Participants
Region of Enrollment
Brazil
13 participants
n=5 Participants
11 participants
n=7 Participants
8 participants
n=5 Participants
32 participants
n=4 Participants

PRIMARY outcome

Timeframe: Pre and post 14 weeks

The relative maximal oxygen uptake was taken by individual connected to a metabolic gas analyzer (VO-2000, Aerosport, Medgraphics, St. Paul, Minnesota) through which the gas samples were collected and measured each 10 seconds during the test. The participants were submitted to a ramp protocol with an initial velocity of 8.0 km/h (0% of inclination), progressive increments, a final velocity of 18 km/h (2% de inclination). The duration was equal to 10 minutes or until voluntary exhaustion.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Relative Maximal Oxygen Uptake
Pre VO2
47.8 mL/(kg*min)
Standard Deviation 6.3
50.9 mL/(kg*min)
Standard Deviation 6.0
49.7 mL/(kg*min)
Standard Deviation 5.6
Relative Maximal Oxygen Uptake
Post VO2
51.7 mL/(kg*min)
Standard Deviation 5.9
52.8 mL/(kg*min)
Standard Deviation 5.1
49.4 mL/(kg*min)
Standard Deviation 6.4

PRIMARY outcome

Timeframe: Pre and post 14 weeks

The absolute maximal oxygen uptake was taken by individual connected to a metabolic gas analyzer (VO-2000, Aerosport, Medgraphics, St. Paul, Minnesota) through which the gas samples were collected and measured each 10 seconds during the test. The participants were submitted to a ramp protocol with an initial velocity of 8.0 km/h (0% of inclination), progressive increments, a final velocity of 18 km/h (2% de inclination). The duration was equal to 10 minutes or until voluntary exhaustion.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Absolute Maximal Oxygen Uptake
Post VO2
4.0 L/min
Standard Deviation 0.6
4.0 L/min
Standard Deviation 0.3
3.7 L/min
Standard Deviation 0.4
Absolute Maximal Oxygen Uptake
Pre VO2
3.67 L/min
Standard Deviation 0.6
3.89 L/min
Standard Deviation 0.3
3.80 L/min
Standard Deviation 0.5

PRIMARY outcome

Timeframe: Pre and post 14 weeks/46 sessions of training

The criterion for determining the total time reaching in maximal VO2 test was associated with the time immediately preceding heart rate shown a reduction of five or more beats.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Total Time Reaching on Maximal Test (tVO2max)
pre tVO2max
9.2 minutes
Standard Deviation 0.8
10.5 minutes
Standard Deviation 1.1
9.6 minutes
Standard Deviation 1.8
Total Time Reaching on Maximal Test (tVO2max)
post tVO2max
10.2 minutes
Standard Deviation 0.7
10.7 minutes
Standard Deviation 1.0
9.5 minutes
Standard Deviation 1.9

PRIMARY outcome

Timeframe: Pre and post 14 weeks at rest (5 min sitting; before start the test), during the test (to determine max), and 60s and 120s immediately after the end of the test (on sitting position)

The HR was collected pre and post training on maximal VO2 test. Initially, with the individual on the treadmill (Inbrasport Master Super, Porto Alegre, Brazil), electrodes (Micromed) were placed at the manubrium, right and left iliac crest for measured heart rate (HR) (derivation CM5) and were connected to an electromyography equipment (Micromed®). HR values were visualized through Elite software (Micromed Biotechnology, Brasilia, Brazil).

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Heart Rate on Maximal Test
pre HR rest
68.8 bpm
Standard Deviation 9.8
67.3 bpm
Standard Deviation 9.6
65.8 bpm
Standard Deviation 8.2
Heart Rate on Maximal Test
post HR rest
59.3 bpm
Standard Deviation 8.6
57.2 bpm
Standard Deviation 2.1
64.4 bpm
Standard Deviation 10.3
Heart Rate on Maximal Test
pre HR max
185.3 bpm
Standard Deviation 11.2
183.5 bpm
Standard Deviation 9.4
190.5 bpm
Standard Deviation 6.0
Heart Rate on Maximal Test
post HR max
187.0 bpm
Standard Deviation 10.6
185.3 bpm
Standard Deviation 10.5
191.0 bpm
Standard Deviation 8.5
Heart Rate on Maximal Test
pre HR 60s
122.5 bpm
Standard Deviation 19.8
116.3 bpm
Standard Deviation 10
122.9 bpm
Standard Deviation 22.8
Heart Rate on Maximal Test
post HR 60s
120.9 bpm
Standard Deviation 10.5
117.5 bpm
Standard Deviation 13.3
123.0 bpm
Standard Deviation 15.3
Heart Rate on Maximal Test
pre HR 120s
110.3 bpm
Standard Deviation 14.4
107.0 bpm
Standard Deviation 9.4
111.6 bpm
Standard Deviation 18.0
Heart Rate on Maximal Test
post HR 120s
108.7 bpm
Standard Deviation 11.1
108.4 bpm
Standard Deviation 13.7
112.3 bpm
Standard Deviation 13.8

PRIMARY outcome

Timeframe: Pre and post 14 weeks/46sessions on rest (5 min sitting position; before start the test); 1-3-5 min immediately after the end of the test (on sitting position).

Capillary blood samples (25 µl) were obtained from the finger of each subject during all tests and the \[La\] were measured using an (Accutrend®Plus Roche Diagnostics Gesellschaft mit beschränkter Haftung , Mannheim, Germany). The measuring range was 0.8-22 millimole (mM). The sample is collecting (Accu-Chek® Softclix) and first applied to a coded yellow test strip (Accutrend Blood measure-Roche) with a reagent chemical substance. Blood was added to the strip by letting it drip from a finger; in accordance with the instrument's instructions. The finger never touched the strip's pad in order to exclude any possible interference due to the sweat. All \[La\] were collected by a single, experienced investigator.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Blood Lactate Concentrations on Maximal Test
post [La] 5-min
13 mmol*L^-1
Standard Deviation 2.58
12.9 mmol*L^-1
Standard Deviation 1.49
13.45 mmol*L^-1
Standard Deviation 1.40
Blood Lactate Concentrations on Maximal Test
pre [La] rest
2.55 mmol*L^-1
Standard Deviation 0.38
2.40 mmol*L^-1
Standard Deviation 0.20
2.36 mmol*L^-1
Standard Deviation 0.23
Blood Lactate Concentrations on Maximal Test
post [La] rest
2.73 mmol*L^-1
Standard Deviation 0.39
2.29 mmol*L^-1
Standard Deviation 0.45
2.68 mmol*L^-1
Standard Deviation 0.23
Blood Lactate Concentrations on Maximal Test
pre [La] 1-min
12.03 mmol*L^-1
Standard Deviation 2.89
12.21 mmol*L^-1
Standard Deviation 2.49
13.41 mmol*L^-1
Standard Deviation 1.54
Blood Lactate Concentrations on Maximal Test
post [La] 1-min
13.14 mmol*L^-1
Standard Deviation 2.57
13.23 mmol*L^-1
Standard Deviation 1.57
13.30 mmol*L^-1
Standard Deviation 1.39
Blood Lactate Concentrations on Maximal Test
pre [La] 3-min
11.42 mmol*L^-1
Standard Deviation 2.23
11.97 mmol*L^-1
Standard Deviation 2.90
12.88 mmol*L^-1
Standard Deviation 1.56
Blood Lactate Concentrations on Maximal Test
post [La] 3-min
12.66 mmol*L^-1
Standard Deviation 2.50
12.88 mmol*L^-1
Standard Deviation 1.65
13.44 mmol*L^-1
Standard Deviation 1.56
Blood Lactate Concentrations on Maximal Test
pre [La] 5-min
9.74 mmol*L^-1
Standard Deviation 3.40
11.5 mmol*L^-1
Standard Deviation 2.74
12.26 mmol*L^-1
Standard Deviation 1.74

PRIMARY outcome

Timeframe: Body Mass was collected pre and post training

To measure body mass Filizola scales with a stadiometer was used.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Body Mass (BM)
post BM
77.5 kg
Standard Deviation 13.7
76.1 kg
Standard Deviation 4.4
76.0 kg
Standard Deviation 11.1
Body Mass (BM)
pre BM
77.6 kg
Standard Deviation 13.5
76.6 kg
Standard Deviation 3.7
76.4 kg
Standard Deviation 10.9

PRIMARY outcome

Timeframe: Pre and post 14 weeks/46 sessions of training

The RPE was collected at maximal test (see description of test on outcome measure "Maximal Oxygen Uptake"). Relative to overall feelings were collected by Category Ratio Scale (CR10) during the last 15 s of each stage using the Borg category (0 - 10) scale. Instructions for RPE were that "0" corresponds to rest, and "9 - 10" corresponds to maximal exertion. This verbal procedure was explained before initiating exercise.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Rate of Perceived Exertion (RPE) on Maximal Test
pre RPE
8.9 units on a scale
Standard Deviation 0.9
9.5 units on a scale
Standard Deviation 0.9
9.2 units on a scale
Standard Deviation 1.0
Rate of Perceived Exertion (RPE) on Maximal Test
post RPE
8.8 units on a scale
Standard Deviation 1.5
9.0 units on a scale
Standard Deviation 1.3
9.0 units on a scale
Standard Deviation 0.9

PRIMARY outcome

Timeframe: Pre and post 14 weeks/46 sessions of training

The subjects underwent a set of anthropometric assessments, which followed the norms of the International Society for Advancement of Kinanthropometry. The fat mass was calculated by percentage using the equation proposed by Jackson and Pollock (1978) from seven skinfold measurement. To measure the skinfolds, a Sanny Professional Skinfold Caliper was used.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
n=8 Participants
The control group did not do the running training program. Control did their normal physical activities.
Fat Mass (%)
pre
14.3 Fat Mass %
Standard Deviation 6.0
11.9 Fat Mass %
Standard Deviation 3.9
12.5 Fat Mass %
Standard Deviation 4.9
Fat Mass (%)
post
14.9 Fat Mass %
Standard Deviation 5.6
13.6 Fat Mass %
Standard Deviation 5.1
13.4 Fat Mass %
Standard Deviation 4.7

SECONDARY outcome

Timeframe: Pre; during (retest I 0-6weeks; retest II 0-10 weeks) and post 14 weeks/46sessions training: rest (5-min sitting; before start the test); maximal during the test (maxHRsub); 60s-120s immediately after the end of the test (on sitting position).

Population: The control group didn't do the submaximal test because it was done to determine and adjusted the training intensities during the 14 weeks.

Submaximal tests were done at the same treadmill (Treadmill® TR9100HR, Life Fitness, USA) as training.Before the test, all subjects should be able to do it at moderate-high intensity. The test began with three minutes of progressive warm-up. During the first minute, the subject maintains a light level; during the second and third minutes of the warm-up, the intensity was moderate. Starting from the fourth minute, the subject should be able to maintain a velocity for the next seven to ten minutes. This load can be adjusted as required, through verbal communication between the subject and the evaluator. The test finishes at the end of 10 minutes.The test velocity should be clearly constant at the end of the test. Heart rate were monitored and recorded at the end of each minute, e.g. 10 seconds before ending each measured minute, and the last measurement were at the end of the 10 minutes. The objective of this procedure was to identify the HR values for training intensities.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
The control group did not do the running training program. Control did their normal physical activities.
Heart Rate on Submaximal Test
pre HR rest
63.9 bpm
Standard Deviation 6.4
67.2 bpm
Standard Deviation 10.8
Heart Rate on Submaximal Test
retest I HR rest
60.8 bpm
Standard Deviation 9.7
64.0 bpm
Standard Deviation 8.5
Heart Rate on Submaximal Test
retest II HR rest
61.2 bpm
Standard Deviation 8.8
59.6 bpm
Standard Deviation 10.7
Heart Rate on Submaximal Test
post HR rest
59.8 bpm
Standard Deviation 8.8
58.7 bpm
Standard Deviation 2.6
Heart Rate on Submaximal Test
pre maxHRsub
174.8 bpm
Standard Deviation 9.6
175.0 bpm
Standard Deviation 8.3
Heart Rate on Submaximal Test
retest I maxHRsub
173.3 bpm
Standard Deviation 10.5
169.8 bpm
Standard Deviation 10.3
Heart Rate on Submaximal Test
retest II maxHRsub
174.3 bpm
Standard Deviation 9.9
173.2 bpm
Standard Deviation 10.0
Heart Rate on Submaximal Test
post maxHRsub
174.3 bpm
Standard Deviation 7.6
170.1 bpm
Standard Deviation 9.7
Heart Rate on Submaximal Test
pre HR 60s
116.2 bpm
Standard Deviation 16.8
116.9 bpm
Standard Deviation 12.5
Heart Rate on Submaximal Test
retest I HR 60s
106.5 bpm
Standard Deviation 14.1
98.3 bpm
Standard Deviation 11.6
Heart Rate on Submaximal Test
retest II HR 60s
105.5 bpm
Standard Deviation 14.0
99.8 bpm
Standard Deviation 14.4
Heart Rate on Submaximal Test
post HR 60s
103.0 bpm
Standard Deviation 11.3
100.7 bpm
Standard Deviation 12.7
Heart Rate on Submaximal Test
pre HR 120s
98.9 bpm
Standard Deviation 14.8
102.3 bpm
Standard Deviation 9.2
Heart Rate on Submaximal Test
retest I HR 120s
90.5 bpm
Standard Deviation 13.4
87.5 bpm
Standard Deviation 10.5
Heart Rate on Submaximal Test
retest II HR 120s
91.9 bpm
Standard Deviation 17.4
90.8 bpm
Standard Deviation 12.3
Heart Rate on Submaximal Test
post HR 120s
93.8 bpm
Standard Deviation 11.9
89.9 bpm
Standard Deviation 9.3

SECONDARY outcome

Timeframe: Pre; during (retest I 0-6weeks; retest II 0-10 weeks) and post 14 weeks/46 sessions training: rest (5-min sitting; before start the test); 1-3-5 minutes immediately after the end of the test (on sitting position).

Population: The control group didn't do the submaximal test because it was done to determine and adjusted the HR training intensities and also collected \[La\] on rest and after submaximal effort during 14 weeks of training.

Capillary blood samples (25 µl) were obtained from the finger of each subject during all tests and the \[La\] were measured using an (Accutrend®Plus Roche Diagnostics Gesellschaft mit beschränkter Haftung , Mannheim, Germany). The measuring range was 0.8-22 mM. The sample is collecting (Accu-Chek® Softclix) and first applied to a coded yellow test strip (Accutrend Blood Measure-Roche) with a reagent chemical substance. Blood was added to the strip by letting it drip from a finger; in accordance with the instrument's instructions we never let the finger touch the strip's pad in order to exclude any possible interference due to the sweat.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
The control group did not do the running training program. Control did their normal physical activities.
Blood Lactate on Submaximal Test
pre [La] rest
2.59 mmol*L^-1
Standard Deviation 0.33
2.40 mmol*L^-1
Standard Deviation 0.22
Blood Lactate on Submaximal Test
retest I [La] rest
2.66 mmol*L^-1
Standard Deviation 0.57
2.45 mmol*L^-1
Standard Deviation 0.39
Blood Lactate on Submaximal Test
retest II [La] rest
2.42 mmol*L^-1
Standard Deviation 0.40
2.25 mmol*L^-1
Standard Deviation 0.44
Blood Lactate on Submaximal Test
post [La] rest
2.81 mmol*L^-1
Standard Deviation 0.39
2.56 mmol*L^-1
Standard Deviation 0.50
Blood Lactate on Submaximal Test
pre [La] 1-min
7.41 mmol*L^-1
Standard Deviation 2.28
9.39 mmol*L^-1
Standard Deviation 2.30
Blood Lactate on Submaximal Test
retest I [La] 1-min
7.52 mmol*L^-1
Standard Deviation 1.81
6.94 mmol*L^-1
Standard Deviation 1.75
Blood Lactate on Submaximal Test
retest II [La] 1-min
8.14 mmol*L^-1
Standard Deviation 2.34
7.89 mmol*L^-1
Standard Deviation 1.50
Blood Lactate on Submaximal Test
post [La] 1-min
8.12 mmol*L^-1
Standard Deviation 2.37
7.69 mmol*L^-1
Standard Deviation 2.10
Blood Lactate on Submaximal Test
pre [La] 3-min
6.96 mmol*L^-1
Standard Deviation 2.43
8.80 mmol*L^-1
Standard Deviation 2.48
Blood Lactate on Submaximal Test
retest I [La] 3-min
6.52 mmol*L^-1
Standard Deviation 1.93
5.65 mmol*L^-1
Standard Deviation 1.05
Blood Lactate on Submaximal Test
retest II [La] 3-min
7.50 mmol*L^-1
Standard Deviation 2.23
7.00 mmol*L^-1
Standard Deviation 1.39
Blood Lactate on Submaximal Test
post [La] 3-min
7.52 mmol*L^-1
Standard Deviation 2.25
7.07 mmol*L^-1
Standard Deviation 2.02
Blood Lactate on Submaximal Test
pre [La] 5-min
5.85 mmol*L^-1
Standard Deviation 2.33
7.21 mmol*L^-1
Standard Deviation 3.18
Blood Lactate on Submaximal Test
retest I [La] 5-min
5.80 mmol*L^-1
Standard Deviation 1.74
5.22 mmol*L^-1
Standard Deviation 1.35
Blood Lactate on Submaximal Test
retest II [La] 5-min
6.91 mmol*L^-1
Standard Deviation 2.34
6.49 mmol*L^-1
Standard Deviation 1.27
Blood Lactate on Submaximal Test
post [La] 5-min
6.79 mmol*L^-1
Standard Deviation 2.03
6.49 mmol*L^-1
Standard Deviation 2.03

SECONDARY outcome

Timeframe: Pre; during (retest I 0-6weeks; retest II 0-10 weeks) and post 14 weeks/46sessions of training

Population: The control group didn't do the submaximal test because it was done to determine and adjusted the HR training intensities and also collected \[Vsub\] during submaximal effort.

Submaximal tests were done at the same treadmill (Treadmill® TR9100HR, Life Fitness, USA) as training.Before the test, all subjects should be able to do it at moderate-high intensity. The test began with three minutes of progressive warm-up. During the first minute, the subject maintains a light level; during the second and third minutes of the warm-up, the intensity was moderate. Starting from the fourth minute, the subject should be able to maintain a velocity for the next seven to ten minutes. This load can be adjusted as required, through verbal communication between the subject and the evaluator. The test finishes at the end of 10 minutes. The test velocity should be clearly constant at the end of the test. Values of velocity were monitored and recorded at the end of each minute, e.g. 10 seconds before ending each measured minute, and the last measurement were at the end of the 10 minutes. The objective of this procedure was to identify the value of that speed has stabilized.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
The control group did not do the running training program. Control did their normal physical activities.
Submaximal Velocity [Vsub]
pre [Vsub]
13.2 km/h
Standard Deviation 2.0
13.9 km/h
Standard Deviation 1.4
Submaximal Velocity [Vsub]
retest I [Vsub]
13.4 km/h
Standard Deviation 0.8
13.7 km/h
Standard Deviation 1.6
Submaximal Velocity [Vsub]
retest II [Vsub]
13.9 km/h
Standard Deviation 0.6
14.4 km/h
Standard Deviation 1.1
Submaximal Velocity [Vsub]
post [Vsub]
13.9 km/h
Standard Deviation 0.8
13.9 km/h
Standard Deviation 0.9

SECONDARY outcome

Timeframe: Pre; during (retest I 0-6weeks; retest II 0-10 weeks) and post 14 weeks/46sessions training

Population: The control group didn't do the submaximal test because it was done only for determine and adjusted the training intensities.

The RPE was collected at submaximal test (see description of test on outcome measure "HR at submaximal test"). Relative to overall feelings were collected by Category Ratio Scale (CR10) during the last 15 s of each stage using the Borg category (0 - 10) scale. Instructions for RPE were that "0" corresponds to rest, and "9 - 10" corresponds to maximal exertion. This verbal procedure was explained before initiating exercise.

Outcome measures

Outcome measures
Measure
Interval
n=13 Participants
The running training for interval group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine. The interval training group intensities ranged between 84-87% HRVO2peak/HRmax-maximal heart rate (vigorous intensity), 88-93%HRVO2peak/HRmax (near maximum intensity) and 94-99% HRVO2peak/HRmax (maximum intensity).
Continuous
n=11 Participants
The running training for continuous group were performed on a treadmill for a period of 14 weeks, with a frequency of three times a week, lasting 20-minutes per session thus completing 46 sessions of training. The intensity and volume of training both were adapted according to the recommendations of the American College of Sports Medicine.The continuous group ran at an intensity of \~87%HRVO2peak/HRmax.
Control
The control group did not do the running training program. Control did their normal physical activities.
Rate of Perceived Exertion (RPE) on Submaximal Test
pre RPE
6 units on a scale
Standard Deviation 1
6 units on a scale
Standard Deviation 2
Rate of Perceived Exertion (RPE) on Submaximal Test
retest I RPE
6 units on a scale
Standard Deviation 1
5 units on a scale
Standard Deviation 1
Rate of Perceived Exertion (RPE) on Submaximal Test
retest II RPE
6 units on a scale
Standard Deviation 2
6 units on a scale
Standard Deviation 2
Rate of Perceived Exertion (RPE) on Submaximal Test
post RPE
7 units on a scale
Standard Deviation 2
7 units on a scale
Standard Deviation 1

Adverse Events

Interval

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Continuous

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Control

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Ph.D. Laura Castro de Garay

University of Trás-os-Montes and Alto Douro

Phone: +55 21 22275084

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place