The Long-term and Short-term Efficacy and Safety of Transplantation Autologous Bone Marrow Cells (BMCs) in Patients With the First STEMI (ST Segment Elevation Myocardial Infarction)

NCT ID: NCT01748383

Last Updated: 2012-12-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE2

Total Enrollment

85 participants

Study Classification

INTERVENTIONAL

Study Start Date

2005-09-30

Study Completion Date

2014-09-30

Brief Summary

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The purpose of this study is to test the hypothesis that the intracoronary transplantation of autologous mononuclear and CD 133 + bone marrow cells will improve left ventricular contractile function and will reduce the combined end points after the primary STEMI (mortality, recurrent myocardial infarction, angina, heart failure, stroke).

Detailed Description

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The study was randomized, opened, controlled. 85 patients with the first STEMI were enrolled. Patients were divided to three groups. On admission all patients were received thrombolytic therapy by 1,5 million U streptokinase. Transplantation of autologous mononuclear bone marrow cells (BMMCs) and аutologous CD133 + cells by balloon catheter placed into infarct-related artery (IRA) was performed at once after stent implantation in 28 patients patients (1st group) and in 10 patients (2nd group) on the 7-21 days of STEMI. Another 47 patients (3nd group) undergo only stent implantation into IRA the same day of STEMI.

Autologous BMMCs were obtained from bone marrow aspirate by gradient centrifugation. Echocardiography, Holter monitoring were performed. Plasma concentration of the pro-inflammatory and anti-inflammatory cytokines (IL1, 6,8,10), of the growth factors (stem cell factor - SCF, vascular endothelial growth factor - VEGF, hepatocyte growth factor - HGF, fibroblast growth factor - FGF, insulin-like growth factor - IGF), the number of circulating CD34 +38-, CD133 +, СD117 +, CD90 +34- stem cells were determined in these patients in the acute and sub-acute myocardial infarction period.

It is going 7 years after the beginning of planned to evaluate left ventricular function of these patients, incidence of cardiovascular end points (death, recurrent myocardial infarction, angina, heart failure, stroke) and their combinations, to evaluate the safety of transplantation of autologous BMCs (formation of intra-myocardial tumor or neoplastic processes of other sites) after 7 years from the beginning of study.

Conditions

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Vascular Diseases Cardiovascular Diseases Acute Myocardial Infarction

Keywords

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Bone marrow cells Acute myocardial infarction Transplantation of autologous mononuclear Transplantation of autologous CD133 + cells

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Transplantation of BMMCs

Autologous BMCs aspiration and transplantation of these cells

Group Type EXPERIMENTAL

Transplantation of BMMCs

Intervention Type PROCEDURE

The wing of the ilium was punctured under the local anesthesia for receiving of autologous BMCs. 100 ml of bone marrow aspirate was taken. BMMCs were obtained by the method of the gradient centrifugation. Autologous BMMCs in the number 93±43 million transplantation by balloon catheter performed into IRA at once after stent implantation.

Transplantation of CD 133+ cells

Autologous CD 133+ BMCs aspiration and transplantation of CD 133+ cells

Group Type EXPERIMENTAL

Transplantation of CD 133+ cells

Intervention Type PROCEDURE

The wing of the ilium was punctured under the local anesthesia for receiving of autologous BMCs. 100 ml of bone marrow aspirate was taken. Autologous CD133 + cells were obtained by the method of the magnetic separation. Phenotyping of the transplanted cells was performed by the cytofluorimetry. Autologous CD 133+ BMCs in the number 5,7 (0,45;9,0) million transplantation by balloon catheter performed into IRA at once after stent implantation.

stenting of IRA

The only stenting of IRA

Group Type ACTIVE_COMPARATOR

stenting of IRA

Intervention Type PROCEDURE

The only stent implantation

Interventions

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Transplantation of BMMCs

The wing of the ilium was punctured under the local anesthesia for receiving of autologous BMCs. 100 ml of bone marrow aspirate was taken. BMMCs were obtained by the method of the gradient centrifugation. Autologous BMMCs in the number 93±43 million transplantation by balloon catheter performed into IRA at once after stent implantation.

Intervention Type PROCEDURE

Transplantation of CD 133+ cells

The wing of the ilium was punctured under the local anesthesia for receiving of autologous BMCs. 100 ml of bone marrow aspirate was taken. Autologous CD133 + cells were obtained by the method of the magnetic separation. Phenotyping of the transplanted cells was performed by the cytofluorimetry. Autologous CD 133+ BMCs in the number 5,7 (0,45;9,0) million transplantation by balloon catheter performed into IRA at once after stent implantation.

Intervention Type PROCEDURE

stenting of IRA

The only stent implantation

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* 18 years and to 75 Years
* Informed consent
* First STEMI
* Term admission to an intensive care unit in the first 24 hours of onset
* Time reperfusion of the IRA is not earlier than 4 hours after the initial onset of acute transmural myocardial infarction

Exclusion Criteria

* Atrial fibrillation, a permanent form Valvular heart disease
* Severe comorbidity
Minimum Eligible Age

18 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Russian Academy of Medical Sciences

OTHER

Sponsor Role lead

Responsible Party

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Vyacheslav Ryabov

Leading researcher of Emergency Cardiology Department

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Vyacheslav Ryabov, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Scientific and Research Institution of Cardiology of Siberian Department of RAMS

Locations

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Scientific and Research Institution of Cardiology of Siberian Department of RAMS

Tomsk, , Russia

Site Status

Countries

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Russia

Other Identifiers

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99

Identifier Type: -

Identifier Source: org_study_id