SAFEGUARD: Pleiotropic Effects of Incretin Based Therapies

NCT ID: NCT01744236

Last Updated: 2015-12-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE4

Total Enrollment

70 participants

Study Classification

INTERVENTIONAL

Study Start Date

2013-04-30

Study Completion Date

2015-08-31

Brief Summary

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The aim of this study is to detail the (mechanisms underlying the) actions of the GLP-1 receptor agonists and DPP-4 inhibitors on the cardiovascular, renal and gastrointestinal systems in patients with Type 2 Diabetes Mellitus.

Detailed Description

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GLP-1 receptors are present in most organ systems of the human body, and pharmacological interventions enhancing GLP-1 activity may influence the function of these organs. The use of GLP-1 receptor agonists (GLP-1RA) and DPP-4 inhibitors (DPP-4i) has been associated with an increased heart rate, acute pancreatitis and acute renal failure. To date, studies in humans detailing the effects of these drugs on these organ systems, biological processes and underlying mechanisms, which could explain these associations, are lacking.

Therefore, as part of the EU-FP7 SAFEGUARD program, the present study will aim to detail the (mechanisms underlying the) actions of GLP-1RA and DPP-4i on the cardiovascular, renal and gastrointestinal system in healthy obese subjects and patients with T2DM.

In the main study, sixty patients with type 2 diabetes will undergo two interventions within the same protocol in order to assess changes in the outcome parameters:

* acute study = acute infusion with exenatide or placebo (to assess the cardiovascular and renal effects)
* long-term study = 12 weeks of treatment with liraglutide, sitagliptin or placebo (to assess the cardiovascular, renal and gastrointestinal effects)

In a substudy (termed 'acute MRI study'), twelve patients with type 2 diabetes will undergo an additional acute intervention study with exenatide (to assess the pancreatic effects)

In a substudy (termed 'pilot-study'), ten healthy obese subjects will undergo a similar acute study like the patients with type 2 diabetes (to assess the cardiovascular and renal effects). Moreover, in these healthy subjects, the effects of exenatide during L-NMMA infusion will be assessed.

Conditions

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Type 2 Diabetes

Keywords

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GLP-1 Receptor Agonists, DPP-4 inhibitors

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Liraglutide (main study, long-term intervention)

This arm (n=20) will receive liraglutide 1.8mg and sitagliptin-placebo during 12 weeks

Group Type EXPERIMENTAL

Liraglutide

Intervention Type DRUG

Liraglutide will be started with a titration period of 2 weeks (week 1: 0.6mg once daily and week 2: 1.2mg once daily). If liraglutide is well tolerated it will be continued for 10 more weeks in a dosage of 1.8mg once daily.

Sitagliptin placebo

Intervention Type DRUG

Sitagliptin-placebo be given once daily for 12 weeks.

Sitagliptin (main study, long-term intervention)

This arm (n=20) will receive sitagliptin 100mg and liraglutide-placebo during 12 weeks

Group Type EXPERIMENTAL

Sitagliptin

Intervention Type DRUG

Sitagliptin 100mg will be given once daily for 12 weeks.

Liraglutide placebo

Intervention Type DRUG

Liraglutide-placebo will be started with a titration period of 2 weeks (week 1: 0.6mg once daily and week 2: 1.2mg once daily). It will be continued for 10 more weeks in a dosage of 1.8mg once daily.

Placebo (main study, long-term intervention)

This arm (n=20) will receive liraglutide-placebo and sitagliptin-placebo during 12 weeks

Group Type PLACEBO_COMPARATOR

Liraglutide placebo

Intervention Type DRUG

Liraglutide-placebo will be started with a titration period of 2 weeks (week 1: 0.6mg once daily and week 2: 1.2mg once daily). It will be continued for 10 more weeks in a dosage of 1.8mg once daily.

Sitagliptin placebo

Intervention Type DRUG

Sitagliptin-placebo be given once daily for 12 weeks.

Exenatide (main study, acute intervention)

Prior to the 12-week intervention study, a GLP-1 receptor agonist (exenatide) will be administered intravenously (n=30).

Group Type EXPERIMENTAL

Exenatide

Intervention Type DRUG

Exenatide will be administered intravenously with a loading dose of 50ng/min for 30 minutes, followed by a maintenance dose of 25ng/min during the rest of the tests

Placebo (main study, acute intervention)

Prior to the 12-week intervention study, placebo will be administered intravenously (n=30).

Group Type PLACEBO_COMPARATOR

Exenatide placebo

Intervention Type DRUG

Exenatide-placebo (saline) will be administered intravenously

Acute MRI intervention study

In a subset of 12 patients with type 2 diabetes, a crossover trial with acute infusion of exenatide and placebo is performed. This is done prior to the 12-week intervention study.

Group Type OTHER

Exenatide

Intervention Type DRUG

Exenatide will be administered intravenously with a loading dose of 50ng/min for 30 minutes, followed by a maintenance dose of 25ng/min during the rest of the tests

Exenatide placebo

Intervention Type DRUG

Exenatide-placebo (saline) will be administered intravenously

Pilot-study

In 10 healthy obese subjects, a crossover trial with acute infusion of exenatide, placebo and L-NMMA is performed.

Group Type OTHER

Exenatide

Intervention Type DRUG

Exenatide will be administered intravenously with a loading dose of 50ng/min for 30 minutes, followed by a maintenance dose of 25ng/min during the rest of the tests

Exenatide placebo

Intervention Type DRUG

Exenatide-placebo (saline) will be administered intravenously

L-NMMA

Intervention Type DRUG

Interventions

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Liraglutide

Liraglutide will be started with a titration period of 2 weeks (week 1: 0.6mg once daily and week 2: 1.2mg once daily). If liraglutide is well tolerated it will be continued for 10 more weeks in a dosage of 1.8mg once daily.

Intervention Type DRUG

Sitagliptin

Sitagliptin 100mg will be given once daily for 12 weeks.

Intervention Type DRUG

Exenatide

Exenatide will be administered intravenously with a loading dose of 50ng/min for 30 minutes, followed by a maintenance dose of 25ng/min during the rest of the tests

Intervention Type DRUG

Liraglutide placebo

Liraglutide-placebo will be started with a titration period of 2 weeks (week 1: 0.6mg once daily and week 2: 1.2mg once daily). It will be continued for 10 more weeks in a dosage of 1.8mg once daily.

Intervention Type DRUG

Sitagliptin placebo

Sitagliptin-placebo be given once daily for 12 weeks.

Intervention Type DRUG

Exenatide placebo

Exenatide-placebo (saline) will be administered intravenously

Intervention Type DRUG

L-NMMA

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Age between 35 and 75 years.
* Females must be post-menopausal (no menses \>1 year).
* Type 2 diabetes (HbA1c 6.5-9% DCCT or 48-75 mmol/mol IFCC), who are being treated with a stable dose of oral antihyperglycemic agents (either metformin alone, SU alone or a combination of metformin and SU) for at least 3 months prior to inclusion.
* BMI 25 - 40 kg/m2
* Caucasian
* Signed informed consent

Exclusion Criteria

* GFR \< 60 mL/min/1.73m2
* Current / chronic use of the following medication: thiazolidinediones, GLP-1RA, DPP-4i, glucocorticoids, NSAIDs, insulin, antimicrobial agents, chemotherapeutics or immune suppressants. Subjects on diuretics will only be excluded when these drugs (e.g. hydrochlorothiazide) cannot be stopped for the duration of the study.
* History of or actual pancreatic disease or impaired pancreatic exocrine function
* Active liver disease
* History of or actual malignancy (with the exception of basal cell carcinoma)
* Current urinary tract infection and active nephritis
* Recent (\<6 months) history of cardiovascular disease, including acute coronary syndrome, stroke, transient ischemic neurologic disorder or chronic heart failure (New York Heart Association grade II-IV)
* Current atrial fibrillation
* Chronic infectious or auto-immune disease
* Substance and/or alcohol abuse
* History of allergy/hypersensitivity to any of the test agents
* Complaints compatible with or established gastroparesis and/or neurogenic bladder
* Any condition that has been recognized as a contra-indication for the use of GLP-1RA and DPP-4i, as listed in the respective SPCs
* History of or actual (severe) mental illness
* Inability to understand the study protocol and/or inability to give informed consent
* History of claustrophobia or presence of metal objects/implants (because of MRI protocol)

For the preceding Pilot study, we will include:

* Males
* Age between 18 and 50 years
* BMI 25 - 40 kg/m2
* Caucasian


* Subjects with a fasting plasma glucose ≥5.6 mmol/L, a 2-hour glucose of ≥7.8 mmol/L after a 75-grams oral glucose tolerance test, or a HbA1c of ≥6.5%
* Subjects using any kind of medication
Minimum Eligible Age

35 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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EU FP7: SAFEGUARD consortium

UNKNOWN

Sponsor Role collaborator

Amsterdam UMC, location VUmc

OTHER

Sponsor Role lead

Responsible Party

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M.H.H. Kramer

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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M.H.H. Kramer, MD PhD

Role: PRINCIPAL_INVESTIGATOR

Amsterdam UMC, location VUmc

Locations

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VU University Medical Center

Amsterdam, , Netherlands

Site Status

Countries

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Netherlands

References

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Smits MM, Fluitman KS, Herrema H, Davids M, Kramer MHH, Groen AK, Belzer C, de Vos WM, Cahen DL, Nieuwdorp M, van Raalte DH. Liraglutide and sitagliptin have no effect on intestinal microbiota composition: A 12-week randomized placebo-controlled trial in adults with type 2 diabetes. Diabetes Metab. 2021 Sep;47(5):101223. doi: 10.1016/j.diabet.2021.101223. Epub 2021 Jan 8.

Reference Type DERIVED
PMID: 33429063 (View on PubMed)

Smits MM, Tonneijck L, Muskiet MH, Kramer MH, Pouwels PJ, Pieters-van den Bos IC, Hoekstra T, Diamant M, van Raalte DH, Cahen DL. Twelve week liraglutide or sitagliptin does not affect hepatic fat in type 2 diabetes: a randomised placebo-controlled trial. Diabetologia. 2016 Dec;59(12):2588-2593. doi: 10.1007/s00125-016-4100-7. Epub 2016 Sep 15.

Reference Type DERIVED
PMID: 27627981 (View on PubMed)

Tonneijck L, Smits MM, Muskiet MH, Hoekstra T, Kramer MH, Danser AH, Ter Wee PM, Diamant M, Joles JA, van Raalte DH. Renal Effects of DPP-4 Inhibitor Sitagliptin or GLP-1 Receptor Agonist Liraglutide in Overweight Patients With Type 2 Diabetes: A 12-Week, Randomized, Double-Blind, Placebo-Controlled Trial. Diabetes Care. 2016 Nov;39(11):2042-2050. doi: 10.2337/dc16-1371. Epub 2016 Sep 1.

Reference Type DERIVED
PMID: 27585605 (View on PubMed)

Smits MM, Tonneijck L, Muskiet MH, Hoekstra T, Kramer MH, Diamant M, Nieuwdorp M, Groen AK, Cahen DL, van Raalte DH. Biliary effects of liraglutide and sitagliptin, a 12-week randomized placebo-controlled trial in type 2 diabetes patients. Diabetes Obes Metab. 2016 Dec;18(12):1217-1225. doi: 10.1111/dom.12748. Epub 2016 Aug 30.

Reference Type DERIVED
PMID: 27451030 (View on PubMed)

Tonneijck L, Smits MM, Muskiet MHA, Hoekstra T, Kramer MHH, Danser AHJ, Diamant M, Joles JA, van Raalte DH. Acute renal effects of the GLP-1 receptor agonist exenatide in overweight type 2 diabetes patients: a randomised, double-blind, placebo-controlled trial. Diabetologia. 2016 Jul;59(7):1412-1421. doi: 10.1007/s00125-016-3938-z. Epub 2016 Apr 1.

Reference Type DERIVED
PMID: 27038451 (View on PubMed)

Smits MM, Tonneijck L, Muskiet MH, Hoekstra T, Kramer MH, Pieters IC, Cahen DL, Diamant M, van Raalte DH. Cardiovascular, renal and gastrointestinal effects of incretin-based therapies: an acute and 12-week randomised, double-blind, placebo-controlled, mechanistic intervention trial in type 2 diabetes. BMJ Open. 2015 Nov 19;5(11):e009579. doi: 10.1136/bmjopen-2015-009579.

Reference Type DERIVED
PMID: 26586327 (View on PubMed)

Related Links

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http://www.safeguard-diabetes.org/

The website of the European project SAFEGUARD

Other Identifiers

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U1111-1130-8248

Identifier Type: OTHER

Identifier Source: secondary_id

2012-003256-36

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

DC2012SAFE001

Identifier Type: -

Identifier Source: org_study_id