Trial Outcomes & Findings for L-Serine Supplementation in Hereditary Sensory Neuropathy Type 1 (NCT NCT01733407)

NCT ID: NCT01733407

Last Updated: 2018-09-12

Results Overview

The Charcot Marie Tooth Neuropathy Score (CMTNS) is a 0 to 36 point composite scoring assessment that is used to measure disease severity in Charcot Marie Tooth Neuropathy and other sensory and motor neuropathies. The CMTNS is composed of 9 items that evaluate functions related to disease progression. These 9 parameters include reviewing sensory symptoms, motor symptoms (arms and legs), pinprick sensibility, vibration, leg strength, arm strength, and nerve conduction tests. Each item is scored from 0 to 4, with the lower scores representing less severe symptoms and higher scores representing more severe symptoms.The 9 individual item scores are then totaled to provide a global measure of disease severity. For example the lowest possible total score is 0 which represents an asymptomatic individual and the highest score possible is a 36 which represents an individual with severe disease progression. There are sub scores that can be assessed but sub scores were not utilized in this study

Recruitment status

COMPLETED

Study phase

PHASE1/PHASE2

Target enrollment

18 participants

Primary outcome timeframe

48 Weeks

Results posted on

2018-09-12

Participant Flow

Participant milestones

Participant milestones
Measure
Sugar Pill
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Overall Study
STARTED
9
9
Overall Study
COMPLETED
7
9
Overall Study
NOT COMPLETED
2
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

L-Serine Supplementation in Hereditary Sensory Neuropathy Type 1

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Total
n=18 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
8 Participants
n=5 Participants
9 Participants
n=7 Participants
17 Participants
n=5 Participants
Age, Categorical
>=65 years
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Age, Continuous
49.9 years
STANDARD_DEVIATION 16.9 • n=5 Participants
45.8 years
STANDARD_DEVIATION 11.0 • n=7 Participants
47.8 years
STANDARD_DEVIATION 14.0 • n=5 Participants
Sex: Female, Male
Female
4 Participants
n=5 Participants
8 Participants
n=7 Participants
12 Participants
n=5 Participants
Sex: Female, Male
Male
5 Participants
n=5 Participants
1 Participants
n=7 Participants
6 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
White
9 Participants
n=5 Participants
9 Participants
n=7 Participants
18 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
8 Participants
n=5 Participants
9 Participants
n=7 Participants
17 Participants
n=5 Participants
Region of Enrollment
Canada
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Charcot-Marie-Tooth Neuropathy Score
24.6 scores on a scale
STANDARD_DEVIATION 7.0 • n=5 Participants
20.6 scores on a scale
STANDARD_DEVIATION 10.0 • n=7 Participants
22.6 scores on a scale
STANDARD_DEVIATION 8.6 • n=5 Participants

PRIMARY outcome

Timeframe: 48 Weeks

The Charcot Marie Tooth Neuropathy Score (CMTNS) is a 0 to 36 point composite scoring assessment that is used to measure disease severity in Charcot Marie Tooth Neuropathy and other sensory and motor neuropathies. The CMTNS is composed of 9 items that evaluate functions related to disease progression. These 9 parameters include reviewing sensory symptoms, motor symptoms (arms and legs), pinprick sensibility, vibration, leg strength, arm strength, and nerve conduction tests. Each item is scored from 0 to 4, with the lower scores representing less severe symptoms and higher scores representing more severe symptoms.The 9 individual item scores are then totaled to provide a global measure of disease severity. For example the lowest possible total score is 0 which represents an asymptomatic individual and the highest score possible is a 36 which represents an individual with severe disease progression. There are sub scores that can be assessed but sub scores were not utilized in this study

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Charcot Marie Tooth Neuropathy Score
25.67 scores on a scale
Standard Deviation 6.69
20.22 scores on a scale
Standard Deviation 10.10

SECONDARY outcome

Timeframe: 48 Weeks

Counts of nerve fibers per unit area in skin biopsies

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Intraepidermal Nerve Fiber Density (IENFD)
Upper Thigh
34.67 nerve fibers per micrometer^2
Standard Deviation 35.12
49.56 nerve fibers per micrometer^2
Standard Deviation 43.44
Intraepidermal Nerve Fiber Density (IENFD)
Lower Calf
0.89 nerve fibers per micrometer^2
Standard Deviation 2.67
13.89 nerve fibers per micrometer^2
Standard Deviation 24.10

SECONDARY outcome

Timeframe: 48 Weeks

Autonomic Function Testing (AFT) tests the effectiveness of your autonomic nervous system which regulates important functions such as blood pressure, heart rate, and respiration. AFT results are quantified using the composite autonomic severity score scale (CASS) which is a scale from 0 to 10 that is the sum of three sub scores (cardiovagal, adrenergic, and sudomotor). Cardiovagal is scored from 0 to 3, sudomotor is scored from 0 to 3, and adrenergic is scored from 0 to 4. The tests include deep breathing, Valsalva maneuver, head-up tilt, and a sweat test. The three subscores are then summed. This total represents the CASS which classifies autonomic function as normal functioning (total score 0), mild (total score 1-3), moderate (total score 4-6), or severe (total score 7-10).

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Autonomic Function Testing (AFT) Composite Autonomic Severity Score (CASS)
3.56 scores on a scale
Standard Deviation 1.24
2.22 scores on a scale
Standard Deviation 1.64

SECONDARY outcome

Timeframe: 48 Weeks

Evaluates the functioning of electrical conduction of the motor and sensory nerves of the human body.

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Nerve Conduction Testing
Motor Right Median (Wrist) Amplitude
3.34 microvolts
Standard Error 4.52
4.21 microvolts
Standard Error 4.29
Nerve Conduction Testing
Motor Right Ulnar (Wrist) Amplitude
2.49 microvolts
Standard Error 3.60
4.37 microvolts
Standard Error 4.80
Nerve Conduction Testing
Sensory Right Superficial Peroneal Amplitude
0.00 microvolts
Standard Error 0.00
0.00 microvolts
Standard Error 0.00
Nerve Conduction Testing
Motor Right Peroneal EDB (Ankle) Amplitude
0.54 microvolts
Standard Error 1.52
0.24 microvolts
Standard Error 0.66
Nerve Conduction Testing
Motor Right Peroneal Tib (Below) Amplitude
0.29 microvolts
Standard Error 0.47
1.39 microvolts
Standard Error 2.15
Nerve Conduction Testing
Sensrory Right Median Amplitude
1.34 microvolts
Standard Error 4.03
5.51 microvolts
Standard Error 9.27
Nerve Conduction Testing
Sensory Right Antebrach Amplitude
2.31 microvolts
Standard Error 3.94
5.89 microvolts
Standard Error 9.54
Nerve Conduction Testing
Sensory Right Superficial Radial Amplitude
4.56 microvolts
Standard Error 10.17
10.84 microvolts
Standard Error 20.11
Nerve Conduction Testing
Sensory Right Sural Amplitude
0.52 microvolts
Standard Error 1.57
1.07 microvolts
Standard Error 2.61

SECONDARY outcome

Timeframe: 48 Weeks

Plasma levels of the deoxysphingoid lipid 1-deoxy-sphinganine measured by liquid chromatography/mass spectrometry after hydrolyzing the N-acyl and O-linked headgroups

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
1-deoxy-sphinganine
0.338 micromole per liter
Standard Deviation 0.191
0.112 micromole per liter
Standard Deviation 0.042

SECONDARY outcome

Timeframe: 48 weeks

Plasma levels of the deoxysphingoid lipid 1-deoxy-sphingosine measured by liquid chromatography/mass spectrometry after hydrolyzing the N-acyl and O-linked headgroups

Outcome measures

Outcome measures
Measure
Sugar Pill
n=9 Participants
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 Participants
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
1-deoxy-sphingosine
0.698 micromole per liter
Standard Deviation 0.306
0.337 micromole per liter
Standard Deviation 0.132

Adverse Events

Sugar Pill

Serious events: 1 serious events
Other events: 9 other events
Deaths: 1 deaths

L-serine

Serious events: 1 serious events
Other events: 9 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Sugar Pill
n=9 participants at risk
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 participants at risk
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Gastrointestinal disorders
Esophageal Cancer
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
MRSA Staff Infection
0.00%
0/9
11.1%
1/9 • Number of events 1

Other adverse events

Other adverse events
Measure
Sugar Pill
n=9 participants at risk
Placebo arm placebo: 400mg/kg/d divided TID for year 1 only.
L-serine
n=9 participants at risk
amino acid supplementation with L-serine L-serine: 400mg/kg/d L-serine or placebo divided TID for year 1, then crossover of placebo arm so that all patients on 400mg/kg/d L-serine divided TID for year 2.
Gastrointestinal disorders
ABDOMINAL PAIN UPPER
0.00%
0/9
11.1%
1/9 • Number of events 1
Gastrointestinal disorders
Diarrhea
11.1%
1/9 • Number of events 1
0.00%
0/9
Gastrointestinal disorders
Dyspepsia
0.00%
0/9
11.1%
1/9 • Number of events 1
Infections and infestations
Cellulitis
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
Clostridium Difficile Infection
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
Influenza
11.1%
1/9 • Number of events 1
11.1%
1/9 • Number of events 1
Infections and infestations
Nail Infection
0.00%
0/9
11.1%
1/9 • Number of events 1
Infections and infestations
Nasopharyngitis
22.2%
2/9 • Number of events 2
22.2%
2/9 • Number of events 2
Infections and infestations
Osteomyelitis
0.00%
0/9
11.1%
1/9 • Number of events 2
Infections and infestations
Pneumonia
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
Sinusitis
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
Staphylococcal Infection
0.00%
0/9
11.1%
1/9 • Number of events 1
Injury, poisoning and procedural complications
Burns Second Degree
0.00%
0/9
11.1%
1/9 • Number of events 1
Injury, poisoning and procedural complications
Foot Fracture
11.1%
1/9 • Number of events 1
11.1%
1/9 • Number of events 1
Injury, poisoning and procedural complications
Frostbite
0.00%
0/9
11.1%
1/9 • Number of events 1
Injury, poisoning and procedural complications
Tendon Injury
0.00%
0/9
11.1%
1/9 • Number of events 1
Injury, poisoning and procedural complications
Thermal Burn
11.1%
1/9 • Number of events 1
0.00%
0/9
Investigations
Blood Sodium Decreased
11.1%
1/9 • Number of events 1
0.00%
0/9
Metabolism and nutrition disorders
Vitamin D Deficiency
0.00%
0/9
11.1%
1/9 • Number of events 1
Musculoskeletal and connective tissue disorders
Bunion
0.00%
0/9
11.1%
1/9 • Number of events 1
Musculoskeletal and connective tissue disorders
Bursitis
11.1%
1/9 • Number of events 1
0.00%
0/9
Musculoskeletal and connective tissue disorders
Pain in Extremity
11.1%
1/9 • Number of events 1
11.1%
1/9 • Number of events 1
Musculoskeletal and connective tissue disorders
Tendonitis
11.1%
1/9 • Number of events 1
0.00%
0/9
Nervous system disorders
Hypoaesthesia
0.00%
0/9
11.1%
1/9 • Number of events 1
Nervous system disorders
Peripheral Sensory Neuropathy
11.1%
1/9 • Number of events 1
0.00%
0/9
Skin and subcutaneous tissue disorders
Blister
0.00%
0/9
11.1%
1/9 • Number of events 1
Skin and subcutaneous tissue disorders
Skin Ulcer
11.1%
1/9 • Number of events 2
11.1%
1/9 • Number of events 1
Surgical and medical procedures
Finger Amputation
0.00%
0/9
11.1%
1/9 • Number of events 1
Vascular disorders
Hypertension
11.1%
1/9 • Number of events 1
0.00%
0/9
Infections and infestations
Localized Infection
11.1%
1/9 • Number of events 1
22.2%
2/9 • Number of events 2

Additional Information

Dr. Florian Eichler

Massachusetts General Hospital

Phone: 617-643-3799

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place