Evaluation of the Allergenicity of AMPHADASE INJECTION (Hyaluronidase Injection USP)

NCT ID: NCT01689363

Last Updated: 2017-01-20

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

253 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-09-30

Study Completion Date

2012-12-31

Brief Summary

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This study is designed to evaluate the allergenicity of Amphadase® in healthy volunteers using an intradermal skin test.

Detailed Description

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Conditions

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Allergic Skin Reaction

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

SCREENING

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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Arm H

Intradermal injection of Amphadase (hyaluronidase 150 USP units/mL)

Group Type EXPERIMENTAL

Hyaluronidase

Intervention Type DRUG

Subjects received intradermal injections of 0.02 mL Amphadase® (hyaluronidase 150 USP units/mL) at two random sites on their forearms.

Arm P

Intradermal injection of Histatrol (histamine base 0.1 mg/mL)

Group Type ACTIVE_COMPARATOR

Histamine

Intervention Type DRUG

Subjects received one intradermal injection of 0.02 mL Histatrol® (histamine base 0.1 mg/mL) at one random sites on their forearms.

Arm N

Intradermal injection of saline (0.02 mL)

Group Type PLACEBO_COMPARATOR

Saline

Intervention Type DRUG

Subjects received one intradermal injection of 0.02 mL saline at one random site on their forearm.

Interventions

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Hyaluronidase

Subjects received intradermal injections of 0.02 mL Amphadase® (hyaluronidase 150 USP units/mL) at two random sites on their forearms.

Intervention Type DRUG

Histamine

Subjects received one intradermal injection of 0.02 mL Histatrol® (histamine base 0.1 mg/mL) at one random sites on their forearms.

Intervention Type DRUG

Saline

Subjects received one intradermal injection of 0.02 mL saline at one random site on their forearm.

Intervention Type DRUG

Other Intervention Names

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Amphadase® Histatrol®

Eligibility Criteria

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Inclusion Criteria

Healthy volunteers of either gender; Those without clinically significant cardiovascular gastrointestinal, hepatic, neurological, psychiatric, endocrine, or other major systemic disease that would unduly risk the subject's safety or interfere with the interpretation of results, assessed according to the judgment of the Principal Investigator. A non-inclusive list which would not be exclusionary and define healthy individual for the purposes of this study are:

* hypothyroidism,
* stable hypertension except those subjects on beta blockers including ocular preparations,
* seasonal/perennial allergic rhinitis if able to wash out of antihistamines,
* stable, mild intermittent asthma (subjects using beta agonists as a monotherapy on an as-needed basis, excluding daily usage),
* migraine if not taking excluded medications,
* mild anxiety/depression if not taking excluded medications, and
* mild arthritic conditions if not taking excluded medications.

* Willingness and ability to sign an informed consent document;
* 18 - 80 years of age;
* Intact skin at the forearm ;
* Female participants are currently practicing effective birth control methods or abstinence.

Exclusion Criteria

* Known allergy, hypersensitivity or contraindications to hyaluronidase, thimerosal, edetate disodium (EDTA);
* Use of medications within a duration considered to interfere with skin testing.
* Known dermographism which may interfere with skin testing.
* Pregnant or lactating women.
Minimum Eligible Age

18 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Amphastar Pharmaceuticals, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Selina Su, MPH

Role: STUDY_CHAIR

A:mphastar Pharmaceuticals, Inc.

Locations

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Amphastar Site 0022

Eugene, Oregon, United States

Site Status

Amphastar Site 0023

Lake Oswego, Oregon, United States

Site Status

Amphastar Site 0026

Portland, Oregon, United States

Site Status

Amphastar Site 0038

Renton, Washington, United States

Site Status

Amphastar Site 0034

Seattle, Washington, United States

Site Status

Countries

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United States

References

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Barr J, Kirkpatrick N, Dick A, Leonard L, Hawksworth G, Noble DW. Effects of adrenaline and hyaluronidase on plasma concentrations of lignocaine and bupivacaine after peribulbar anaesthesia. Br J Anaesth. 1995 Dec;75(6):692-7. doi: 10.1093/bja/75.6.692.

Reference Type BACKGROUND
PMID: 8672315 (View on PubMed)

Brydon CW, Basler M, Kerr WJ. An evaluation of two concentrations of hyaluronidase for supplementation of peribulbar anaesthesia. Anaesthesia. 1995 Nov;50(11):998-1000. doi: 10.1111/j.1365-2044.1995.tb05940.x.

Reference Type BACKGROUND
PMID: 8678264 (View on PubMed)

Ahluwalia HS, Lukaris A, Lane CM. Delayed allergic reaction to hyaluronidase: a rare sequel to cataract surgery. Eye (Lond). 2003 Mar;17(2):263-6. doi: 10.1038/sj.eye.6700243. No abstract available.

Reference Type BACKGROUND
PMID: 12640426 (View on PubMed)

Agrawal A, McLure HA, Dabbs TR. Allergic reaction to hyaluronidase after a peribulbar injection. Anaesthesia. 2003 May;58(5):493-4. doi: 10.1046/j.1365-2044.2003.03154_17.x. No abstract available.

Reference Type BACKGROUND
PMID: 12694021 (View on PubMed)

Kempeneers A, Dralands L, Ceuppens J. Hyaluronidase induced orbital pseudotumor as complication of retrobulbar anesthesia. Bull Soc Belge Ophtalmol. 1992;243:159-66.

Reference Type BACKGROUND
PMID: 1302146 (View on PubMed)

Minning CA Jr. Hyaluronidase allergy simulating expulsive choroidal hemorrhage. Arch Ophthalmol. 1994 May;112(5):585-6. doi: 10.1001/archopht.1994.01090170029012. No abstract available.

Reference Type BACKGROUND
PMID: 8185511 (View on PubMed)

Szepfalusi Z, Nentwich I, Dobner M, Pillwein K, Urbanek R. IgE-mediated allergic reaction to hyaluronidase in paediatric oncological patients. Eur J Pediatr. 1997 Mar;156(3):199-203. doi: 10.1007/s004310050582.

Reference Type BACKGROUND
PMID: 9083759 (View on PubMed)

Allergy testing. American College of Physicians. Ann Intern Med. 1989 Feb 15;110(4):317-20. No abstract available.

Reference Type BACKGROUND
PMID: 2578014 (View on PubMed)

Poulsen LK. In vivo and in vitro techniques to determine the biological activity of food allergens. J Chromatogr B Biomed Sci Appl. 2001 May 25;756(1-2):41-55. doi: 10.1016/s0378-4347(01)00070-6.

Reference Type BACKGROUND
PMID: 11419727 (View on PubMed)

Howard A, Mercer P, Nataraj HC, Kang BC. Bevel-down superior to bevel-up in intradermal skin testing. Ann Allergy Asthma Immunol. 1997 Jun;78(6):594-6. doi: 10.1016/s1081-1206(10)63222-x.

Reference Type BACKGROUND
PMID: 9207725 (View on PubMed)

Barbaud A, Reichert-Penetrat S, Trechot P, Jacquin-Petit MA, Ehlinger A, Noirez V, Faure GC, Schmutz JL, Bene MC. The use of skin testing in the investigation of cutaneous adverse drug reactions. Br J Dermatol. 1998 Jul;139(1):49-58. doi: 10.1046/j.1365-2133.1998.02313.x.

Reference Type BACKGROUND
PMID: 9764148 (View on PubMed)

Fisher MM, Bowey CJ. Intradermal compared with prick testing in the diagnosis of anaesthetic allergy. Br J Anaesth. 1997 Jul;79(1):59-63. doi: 10.1093/bja/79.1.59.

Reference Type BACKGROUND
PMID: 9301390 (View on PubMed)

Ruzicka T, Gerstmeier M, Przybilla B, Ring J. Allergy to local anesthetics: comparison of patch test with prick and intradermal test results. J Am Acad Dermatol. 1987 Jun;16(6):1202-8. doi: 10.1016/s0190-9622(87)70158-3.

Reference Type BACKGROUND
PMID: 3597862 (View on PubMed)

Other Identifiers

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API-H001-CLN-A2

Identifier Type: -

Identifier Source: org_study_id

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