Efficacy Of Tocotrienol a Natural Vitamin E In Biopsy Wound

NCT ID: NCT01579227

Last Updated: 2018-01-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

101 participants

Study Classification

OBSERVATIONAL

Study Start Date

2012-01-31

Study Completion Date

2018-12-31

Brief Summary

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The following two objectives are proposed in healthy subjects to characterize (1) wound closure, (2) scar formation/appearance, and (3) inflammatory response:

Objective 1, (topical only - referred to as "TOP") - Topical application of Tocotrienol (TCT) vs placebo in bilateral punch biopsy

Objective 2, (oral and topical - referred to as "OTOP") - Combined oral supplementation and topical application of tocotrienol (TCT) vs placebo in bilateral punch biopsy

Objective 3, (topical only - referred to as "TAM") - Topical application of tamoxifen vs placebo in bilateral punch biopsy.

Objective 4, (topical only to normal skin) - Topical application of TCT vs placebo on bilateral lets on normal skin.

Detailed Description

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* In nature, the vitamin E family is split into two classes: tocopherols (TCP) and tocotrienols (TCT). Members of the TCP and TCT family are biologically unique.

* TCP are mainly found in green leafy vegetables while TCT are the primary vitamin E of seeds, including cereal grains such as wheat, rice, and barley.
* Vitamin E is thought to improve wound healing by inhibiting collagen synthesis and attenuating fibroblast proliferation and inflammation. However, outcomes based scientific literature on the therapeutic efficacy of vitamin E in skin wound closure is scant and has primarily focused on TCP.

* Oral supplementation of TCP showed modest improvement in rodent wound closure, but the relevance of oral TCP supplementation in rats already receiving high dose vitamin E in a standard laboratory is questionable.
* Topical TCP on surgical wounds of children have been shown to improve wound healing; yet no mechanistic basis for the observed effect was described.
* Preliminary observations from the PI's active IRB protocol to test TCT in scar appearance of surgical wounds led us to evaluate the potential of TCT vitamin E to improve wound closure in healthy subjects. To date, the therapeutic efficacy of TCT in either topical (TOP) or oral with topical (OTOP) applications for skin wound healing remains to be reported.

* Preliminary observations also made show down-regulation of microRNA-200b supports cutaneous angiogenesis, the most important step in cutaneous wound healing. Tamoxifen silences mircroRNA-200b and later work has recognized that under non-neoplastic conditions, tamoxifen may induce angiogenesis.

Conditions

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Scar

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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1-TOP group 1

TOP group 1 will have biopsy #2 collected 3 days after 1st biopsy collected.

No interventions assigned to this group

TOP group 2

TOP group 2 will have biopsy #2 collected 30 days after 1st biopsy collected.

No interventions assigned to this group

OTOP group-1

OTOP will use topical cream (either topical tocotrienol (TCT) or placebo cream) as well as oral supplementation (either oral Tocotrienol capsules (TCT) or placebo capsules). OTOP group 1 will have biopsy #2 collected 3 days after 1st biopsy collected.

Topical Tocotrienol (TCT)

Intervention Type DEVICE

Natural Form Vitamin E topical cream(TCT)

Oral Tocotrienol Capsules (TCT)

Intervention Type DIETARY_SUPPLEMENT

Natural Form Vitamin E Oral capsules (TCT)

Placebo cream

Intervention Type DEVICE

Placebo cream

placebo capsule

Intervention Type DIETARY_SUPPLEMENT

placebo capsule

OTOP group-2

OTOP will use topical cream (either topical tocotrienol (TCT) or placebo cream) as well as oral supplementation (either oral Tocotrienol capsules (TCT) or placebo capsules). OTOP group 2 will have biopsy #2 collected 30 days after 1st biopsy collected.

Topical Tocotrienol (TCT)

Intervention Type DEVICE

Natural Form Vitamin E topical cream(TCT)

Oral Tocotrienol Capsules (TCT)

Intervention Type DIETARY_SUPPLEMENT

Natural Form Vitamin E Oral capsules (TCT)

Placebo cream

Intervention Type DEVICE

Placebo cream

placebo capsule

Intervention Type DIETARY_SUPPLEMENT

placebo capsule

TAM Group 1

TAM group 1 will have #2 biopsy collected 21 days after 1st biopsy collected. Tamoxifen cream and placebo cream will be applied where biopsies are collected from 1 week prior to having the biopsy procedure until the second biopsy is collected (21 days later).

Tamoxifen Cream

Intervention Type DEVICE

Tamoxifen cream

Normal Skin

Placebo group will apply placebo and TCT cream that will be applied daily to a specified area on the subjects legs (normal skin) for 5 weeks. One leg will be applied with placebo and the other will be applied with TCT cream. Subjects will return weekly for 5 weeks, where non-invasive measurements using Laser Speckle imaging, will be completed at each study visit

Tamoxifen Cream

Intervention Type DEVICE

Tamoxifen cream

Placebo cream

Intervention Type DEVICE

Placebo cream

Interventions

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Topical Tocotrienol (TCT)

Natural Form Vitamin E topical cream(TCT)

Intervention Type DEVICE

Oral Tocotrienol Capsules (TCT)

Natural Form Vitamin E Oral capsules (TCT)

Intervention Type DIETARY_SUPPLEMENT

Tamoxifen Cream

Tamoxifen cream

Intervention Type DEVICE

Placebo cream

Placebo cream

Intervention Type DEVICE

placebo capsule

placebo capsule

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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TCT TCT Placebo placebo

Eligibility Criteria

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Inclusion Criteria

* Ages- 18-50 (Both Male \& Female)
* Non-smoker - having quit at least 3 months prior to enrollment
* Non-diabetic
* Non-pregnant or non-breastfeeding - verbal assent.
* If a female subject of childbearing age misses her menstrual period after the start of the study, she will inform the investigators and be given a pregnancy test to ensure, for safety reasons, that she is not pregnant. If she is pregnant, she will discontinue participation in the study.
* No current use of OTC medications or other form of supplements containing vitamin-E

Exclusion Criteria

* Diabetes or HIV diagnosis
* Alcohol or drug abuse
* unable to provide informed consent
* Therapeutically anti-coagulated
* Prisoner
* Currently prescribed immunosuppressant medication
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Carotech Inc.

INDUSTRY

Sponsor Role collaborator

Chandan K Sen

OTHER

Sponsor Role lead

Responsible Party

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Chandan K Sen

Professor

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Chandan K Sen, PhD

Role: PRINCIPAL_INVESTIGATOR

Ohio State University

Locations

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The Ohio State University Medical Center

Columbus, Ohio, United States

Site Status

Countries

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United States

Other Identifiers

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2011H0286

Identifier Type: -

Identifier Source: org_study_id

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