Combination Chemotherapy, Monoclonal Antibody, and Natural Killer Cells in Treating Young Patients With Recurrent or Refractory Neuroblastoma

NCT ID: NCT01576692

Last Updated: 2018-11-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

34 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-04-30

Study Completion Date

2018-10-17

Brief Summary

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This is a safety / feasibility trial evaluating the combination of a humanized anti-GD2 antibody (HU14.18K322A) manufactured at the Children's GMP, LLC at St. Jude with allogeneic natural killer (NK) cells and standard chemotherapy in children with relapsed or refractory neuroblastoma.

Detailed Description

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Eligible participants will receive chemotherapy combined with Hu14.18K322A antibody daily for four consecutive days. Those participants who go on to receive the second course of chemotherapy with Hu14.18K322A will receive an infusion of allogeneic NK cells after the 4th dose of Hu14.18K322A antibody. A maximum of six courses will be given.

Primary Objective:

* To observe and describe the toxicities associated with humanized anti-GD2 antibody (hu14.18K322A) with and without allogeneic NK cells when given with repeated cycles of chemotherapy to children with refractory/relapsed neuroblastoma.

Secondary Objective:

* To describe response, time to progression, event-free and overall survival.
* To evaluate the feasibility of administering NK cells from a suitable donor after completion of the last dose of hu14.18K322A in three repeated cycles of chemotherapy

Conditions

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Neuroblastoma

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Treatment

Participants receive humanized anti-GD2 antibody, chemotherapy, cytokines, and natural killer cells.

Cells for infusion are prepared using the CliniMACS System.

Group Type EXPERIMENTAL

Humanized anti-GD2 antibody

Intervention Type BIOLOGICAL

A maximum of 6 courses of therapy may be given on the following schedule:

* Courses 1, 3, and 5: Humanized anti-GD2 antibody + chemotherapy
* Courses 2, 4, and 6: Humanized anti-GD2 antibody + chemotherapy, with or without natural killer (NK) cells (depending on availability of appropriate NK donor)
* Humanized anti-GD2 antibody (hu14.18 K322A) dosage: 40 mg/m\^2)/dose, over 4 hours daily
* NK Cell dosage: minimum of 0.1 \* 10\^6 cells/kg; maximum of 400 \* 10\^6 CD45+ cells/kg, given once

Chemotherapy

Intervention Type DRUG

Chemotherapy may include the following at the dosages shown below:

* cyclophosphamide: 400 mg/m\^2 IV days 1-5
* topotecan: 1.2 mg/m\^2 IV days 1-5
* temozolomide: 150 mg/m\^2 PO at least 1 hour before irinotecan
* irinotecan: 50 mg/m\^2 IV over 1 hour daily for 5 days
* carboplatin (AUC 8-dosing based on GFR), IV day 1 only
* ifosfamide: 2 g/m2 IV days 1-3
* etoposide: 100 mg/m2 days 1-3

Cytokines

Intervention Type OTHER

Cytokines may be given at the following dosages:

* interleukin-2: 1 million units/m\^2 SQ beginning day 6 of each chemotherapy course and continued every other day for 6 doses
* GM-CSF: 250 mcg/m\^2/day beginning on day 7 or day 8 of chemotherapy course and continued daily through the nadir until ANC \>2,000/mm\^3

Natural killer cells

Intervention Type BIOLOGICAL

NK cells from haploidentical family donor will be infused on day 7 or 8, depending on course. NK cells may be infused in either the inpatient or outpatient setting by a physician, Physician Assistant, Nurse Practitioner, or qualified RN. Careful monitoring and supportive care during NK cell infusion will be guided in part by the Standard Operating Procedures for Lymphocytes Infusions in the St. Jude Nursing Policy \& Procedure Manual.

CliniMACS

Intervention Type DEVICE

The mechanism of action of the CliniMACS Cell Selection System is based on magnetic-activated cell sorting (MACS). The CliniMACS device is a powerful tool for the isolation of many cell types from heterogeneous cell mixtures, (e.g. apheresis products). These can then be separated in a magnetic field using an immunomagnetic label specific for the cell type of interest, such as CD3+ human T cells.

Interventions

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Humanized anti-GD2 antibody

A maximum of 6 courses of therapy may be given on the following schedule:

* Courses 1, 3, and 5: Humanized anti-GD2 antibody + chemotherapy
* Courses 2, 4, and 6: Humanized anti-GD2 antibody + chemotherapy, with or without natural killer (NK) cells (depending on availability of appropriate NK donor)
* Humanized anti-GD2 antibody (hu14.18 K322A) dosage: 40 mg/m\^2)/dose, over 4 hours daily
* NK Cell dosage: minimum of 0.1 \* 10\^6 cells/kg; maximum of 400 \* 10\^6 CD45+ cells/kg, given once

Intervention Type BIOLOGICAL

Chemotherapy

Chemotherapy may include the following at the dosages shown below:

* cyclophosphamide: 400 mg/m\^2 IV days 1-5
* topotecan: 1.2 mg/m\^2 IV days 1-5
* temozolomide: 150 mg/m\^2 PO at least 1 hour before irinotecan
* irinotecan: 50 mg/m\^2 IV over 1 hour daily for 5 days
* carboplatin (AUC 8-dosing based on GFR), IV day 1 only
* ifosfamide: 2 g/m2 IV days 1-3
* etoposide: 100 mg/m2 days 1-3

Intervention Type DRUG

Cytokines

Cytokines may be given at the following dosages:

* interleukin-2: 1 million units/m\^2 SQ beginning day 6 of each chemotherapy course and continued every other day for 6 doses
* GM-CSF: 250 mcg/m\^2/day beginning on day 7 or day 8 of chemotherapy course and continued daily through the nadir until ANC \>2,000/mm\^3

Intervention Type OTHER

Natural killer cells

NK cells from haploidentical family donor will be infused on day 7 or 8, depending on course. NK cells may be infused in either the inpatient or outpatient setting by a physician, Physician Assistant, Nurse Practitioner, or qualified RN. Careful monitoring and supportive care during NK cell infusion will be guided in part by the Standard Operating Procedures for Lymphocytes Infusions in the St. Jude Nursing Policy \& Procedure Manual.

Intervention Type BIOLOGICAL

CliniMACS

The mechanism of action of the CliniMACS Cell Selection System is based on magnetic-activated cell sorting (MACS). The CliniMACS device is a powerful tool for the isolation of many cell types from heterogeneous cell mixtures, (e.g. apheresis products). These can then be separated in a magnetic field using an immunomagnetic label specific for the cell type of interest, such as CD3+ human T cells.

Intervention Type DEVICE

Other Intervention Names

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Hu14.18K322A monoclonal antibody Cytoxan Hycamtin Temodar Camptosar Paraplatin Ifex VP-16 Vepesid IL-2 Aldesleukin Proleukin Sargramostim Leukine NK cells Cell Selection System

Eligibility Criteria

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Inclusion Criteria

* Diagnosis of recurrent or refractory neuroblastoma.
* Age \< 22 years at the time of enrollment.
* Measurable or evaluable (detectable by bone scan or MIBG, but not measurable) disease.
* Organ function: Must have adequate organ and marrow function as defined by the following parameters:

* Bone marrow: Absolute neutrophil count (ANC) \> 750/mm3; Platelets \> 75,000/mm3 (no platelet transfusions for at least 1 week)
* Hepatic: Total bilirubin ≤ 2 x upper limit of normal (ULN) for age; SGPT (ALT) ≤ 2.5 x ULN for age.
* Renal: Creatinine clearance or radioisotope GFR equal to or \>70 ml/min/1.73m2 OR serum creatinine based on age as follows:

* Age 5 years of age and under, then maximum serum creatinine 0.8 mg/dL
* Age \>5 and equal to or \<10 years, then maximum serum creatinine 1.0 mg/dL
* Age \>10 and equal to or \<15 years, then maximum serum creatinine 1.2 mg/dL
* Age \>15 years, then maximum serum creatinine 1.5 mg/dL
* Cardiovascular: Shortening fraction \> or equal to 27% by echocardiogram; Corrected QT interval \< or equal to 450 milliseconds
* Performance status: Karnofsky \> or equal to 50 for \> 10 years of age; Lansky \> or equal to 50 for children equal to or \< 10 years of age.
* Prior therapy: Participant must have fully recovered from the acute toxic effects of all prior therapy prior to enrolling on study.

* Myelosuppressive chemotherapy: Must not have received myelosuppressive therapy within 2 weeks prior to study entry (4 weeks if nitrosurea).
* Biologic (anti-neoplastic agent): At least 7 days since the completion of therapy with biologic agent, including retinoic acid. At least 6 weeks must have elapsed since prior therapy that includes a monoclonal antibody. Participants receiving IVIG are eligible; however, participant must not receive IVIG during the 4 days of antibody infusion.
* Radiation therapy: At least 2 weeks since prior local radiation therapy at the time of study entry.
* Growth factors: Must not have received hematopoietic growth factors (G-CSF, GM-CSF) for at least 1 week prior to study entry.
* Investigational agent: Must not have received investigational agent within 7 days of study entry.
* Immune therapy: Must not have received immunosuppressive (including glucocorticoids), immunostimulatory or any immunomodulatory treatment within 2 weeks of study entry. Steroid containing inhalers, steroid replacement for adrenal insufficiency and steroid premedication for prevention of transfusion or imaging contrast-agent related allergic reaction will be permitted.
* Participants may have had prior CNS metastasis providing: CNS disease has been previously treated and CNS disease has been clinically stable for 4 weeks prior to study entry (assessment must be made by CT or MRI).
* Written informed consent following institutional and federal guidelines.


* Donor is a partially matched family member.
* Donor is HIV negative.
* Donor is at least 18 years of age.
* Donor is not pregnant.
* Donor does not have any other medical condition that, in the opinion of an independent physician, precludes performance of an apheresis procedure.

Exclusion Criteria

* Prior monoclonal antibody: Participants having received in vivo monoclonal anti-GD2 antibodies for biologic therapy or for tumor imaging are ineligible if they have experienced a severe allergic reaction while receiving prior anti-GD2 therapy.
* Pregnancy or breast feeding: Study participants who are pregnant are not eligible for this study. Pregnancy tests must be obtained in girls who are \> 10 years of age or post-menarchal within 7 days prior to study enrollment. Males or females of reproductive potential may not participate unless they have agreed to use an effective contraceptive method during participation in the trial. Breast feeding should be discontinued if a mother wishes to participate in this study.
* Allergy: Known hypersensitivity to other recombinant human antibodies.
* An uncontrolled infection.
* Participants who have not started protocol therapy within 7 days of study enrollment.
Maximum Eligible Age

21 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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CURE Childhood Cancer, Inc.

OTHER

Sponsor Role collaborator

St. Jude Children's Research Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Wayne L. Furman, MD

Role: PRINCIPAL_INVESTIGATOR

St. Jude Children's Research Hospital

Locations

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St. Jude Children's Research Hospital

Memphis, Tennessee, United States

Site Status

Countries

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United States

Related Links

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http://www.stjude.org

St. Jude Children's Research Hospital

http://www.stjude.org/protocols

Clinical Trials Open at St. Jude

Other Identifiers

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NCI-2012-00495

Identifier Type: REGISTRY

Identifier Source: secondary_id

GD2NK

Identifier Type: -

Identifier Source: org_study_id

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