Low Dose β-carotene Supplementation Diminishes Oxidative Stress in Type 2 Diabetics and Healthy Individuals

NCT ID: NCT01477112

Last Updated: 2011-11-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

117 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-01-31

Study Completion Date

2010-12-31

Brief Summary

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Since diabetes has multiple etiologies and oxidative stress one of the proposed mechanisms, the objective is to determine the effect of supplementation with β-carotene to type 2 diabetics and healthy individuals, on iron metabolism, oxidative balance, and antioxidant plasma capacity, using doses similar to the daily nutritional requirement.

Detailed Description

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Type 2 diabetes is a chronic, multifactorial disease, and oxidative stress one of the pathophysiological mechanisms associated with its appearance and development. The objective was to determine the effect of supplementation with β-carotene to type 2 diabetics and healthy individuals, on iron metabolism, oxidative balance, and antioxidant plasma capacity, using doses similar to the daily nutritional requirement.

A total of 117 volunteers participated in the study. Type 2 diabetics (34) and healthy individuals (24), received 6 mg β-carotene for 45 d, and were compared to similar non-supplemented diabetic (33) and control (26) groups. Blood samples were taken at the beginning, end and 30 days after finishing supplementation, to determine hemoglobin, hematocrit unsaturated iron binding capacity, total iron binding capacity, transferrin saturation, ferritin, glycemia, glycosylated hemoglobin, cholesterol, triglycerides, HDL, LDL, oxidized LDL, copper, zinc, TBARS, FRAP, nitrites, GPx, SOD, folates, retinol and β-carotene.

Conditions

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Diabetes Mellitus Oxidative Stress Iron Metabolism Disorders

Keywords

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Type 2 diabetes β-carotene Oxidative balance Oxidants Antioxidants

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Supplemented Diabetics (DB)

Diabetics supplemented with betacarotene for 45 days

Group Type EXPERIMENTAL

Betacarotene

Intervention Type DIETARY_SUPPLEMENT

6 mg betacarotene in caplets for 45 days (daily)and reevaluate parameters 30 days after finishing supplementation

Unsupplemented Diabetics (DS)

Diabetics without betacarotene supplementation

Group Type EXPERIMENTAL

Controls. No treatment

Intervention Type DIETARY_SUPPLEMENT

Evaluate at time 0, 45 days and 75 days, but without receiving betacarotene supplements

Supplemented Controls (CB)

Controls supplemented with betacarotene for 45 days

Group Type ACTIVE_COMPARATOR

Betacarotene

Intervention Type DIETARY_SUPPLEMENT

6 mg betacarotene in caplets for 45 days (daily)and reevaluate parameters 30 days after finishing supplementation

Unsupplemented Controls (CS)

Controls without betacarotene supplementation

Group Type ACTIVE_COMPARATOR

Controls. No treatment

Intervention Type DIETARY_SUPPLEMENT

Evaluate at time 0, 45 days and 75 days, but without receiving betacarotene supplements

Interventions

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Betacarotene

6 mg betacarotene in caplets for 45 days (daily)and reevaluate parameters 30 days after finishing supplementation

Intervention Type DIETARY_SUPPLEMENT

Controls. No treatment

Evaluate at time 0, 45 days and 75 days, but without receiving betacarotene supplements

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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β-carotene as a soft gel capsule (GNC, Pennsylvania-USA) No supplements

Eligibility Criteria

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Inclusion Criteria

Patients with a diagnose of Type 2 diabetes mellitus of at least 5 years of diagnosis, in treatment with oral hypoglycemics Patients in regular control (once a month) in the Hospital

Exclusion Criteria

* Hospitalized patient
* Diabetic patient with diabetes related acute complications (ketoacidosis, hyperosmolar coma)in the 3 months previous to the study.
* Individuals with infections that required antibiotics in the 3 weeks previous to the study.
* Individuals with antibodies anti-insulin, autoimmune diseases or in treatment with immunosuppressive drugs.
* Individuals with viral infections such as hepatitis B, hematological, renal or hepatic diseases.
* Pregnancy
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Seguros Caracas Foundation

UNKNOWN

Sponsor Role collaborator

National Fund for Science and Technology, Science Mission

UNKNOWN

Sponsor Role collaborator

Instituto Venezolano de Investigaciones Cientificas

OTHER

Sponsor Role lead

Responsible Party

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Maria Nieves Garcia-Casal

Director of Pathophysiology Laboratory

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Maria N Garcia-Casal, PhD

Role: STUDY_DIRECTOR

Instituto Venezolano de Investigaciones Cientificas

Jose M Moreno, PhD

Role: PRINCIPAL_INVESTIGATOR

Instituto Venezolanode Investigaciones cientificas

Locations

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Hospital Baudilio Lara

Barquisimeto, Lara, Venezuela

Site Status

Countries

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Venezuela

References

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Ford ES, Cogswell ME. Diabetes and serum ferritin concentration among U.S. adults. Diabetes Care. 1999 Dec;22(12):1978-83. doi: 10.2337/diacare.22.12.1978.

Reference Type BACKGROUND
PMID: 10587829 (View on PubMed)

Ford ES, Mokdad AH, Giles WH, Brown DW. The metabolic syndrome and antioxidant concentrations: findings from the Third National Health and Nutrition Examination Survey. Diabetes. 2003 Sep;52(9):2346-52. doi: 10.2337/diabetes.52.9.2346.

Reference Type BACKGROUND
PMID: 12941775 (View on PubMed)

Sugiura M, Nakamura M, Ikoma Y, Yano M, Ogawa K, Matsumoto H, Kato M, Ohshima M, Nagao A. The homeostasis model assessment-insulin resistance index is inversely associated with serum carotenoids in non-diabetic subjects. J Epidemiol. 2006 Mar;16(2):71-8. doi: 10.2188/jea.16.71.

Reference Type BACKGROUND
PMID: 16537987 (View on PubMed)

Song Y, Cook NR, Albert CM, Van Denburgh M, Manson JE. Effects of vitamins C and E and beta-carotene on the risk of type 2 diabetes in women at high risk of cardiovascular disease: a randomized controlled trial. Am J Clin Nutr. 2009 Aug;90(2):429-37. doi: 10.3945/ajcn.2009.27491. Epub 2009 Jun 2.

Reference Type BACKGROUND
PMID: 19491386 (View on PubMed)

Related Links

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http://www.diabetes.org

American Diabetes Association

Other Identifiers

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IVIC-HUMNUT-001

Identifier Type: -

Identifier Source: org_study_id