Trial Outcomes & Findings for Pharmacokinetic Study to Compare the Blood Levels of Abatacept Manufactured at Lonza Biologics to the Blood Levels of Abatacept Manufactured at the Devens, Massachusetts (MA) Facility of Bristol-Myers Squibb (NCT NCT01439204)

NCT ID: NCT01439204

Last Updated: 2014-03-19

Results Overview

Cmax was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). Cmax was measured in micro grams per milliliter (µg/mL).

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

223 participants

Primary outcome timeframe

Days 1 to 71

Results posted on

2014-03-19

Participant Flow

10 October 2011 to 11 Feb 2012. Participants who were randomized to an arm were admitted to a clinical facility the day prior to dosing (Day -1) and confined until 24 hours post the single dose; participants followed to Day 71.

Healthy participants who weighed between 60 and 100 kilograms, inclusive. 223 enrolled; 72 randomized to an arm. Reasons for not being randomized: 24 withdrew consent, 5 poor/non-compliance, 111 no longer met study criteria, 11 other. Participants were age and sex matched between treatment arms.

Participant milestones

Participant milestones
Measure
750 mg Abatacept From Lonza, NH
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Overall Study
STARTED
36
36
Overall Study
COMPLETED
32
33
Overall Study
NOT COMPLETED
4
3

Reasons for withdrawal

Reasons for withdrawal
Measure
750 mg Abatacept From Lonza, NH
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Overall Study
Withdrawal by Subject
0
1
Overall Study
Lost to Follow-up
0
2
Overall Study
No longer meets study criteria
1
0
Overall Study
poor/non-compliance
3
0

Baseline Characteristics

Pharmacokinetic Study to Compare the Blood Levels of Abatacept Manufactured at Lonza Biologics to the Blood Levels of Abatacept Manufactured at the Devens, Massachusetts (MA) Facility of Bristol-Myers Squibb

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Total
n=72 Participants
Total of all reporting groups
Age, Continuous
31.6 years
STANDARD_DEVIATION 10.02 • n=93 Participants
31.2 years
STANDARD_DEVIATION 8.90 • n=4 Participants
31.4 years
STANDARD_DEVIATION 9.41 • n=27 Participants
Sex: Female, Male
Female
22 Participants
n=93 Participants
21 Participants
n=4 Participants
43 Participants
n=27 Participants
Sex: Female, Male
Male
14 Participants
n=93 Participants
15 Participants
n=4 Participants
29 Participants
n=27 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
4 Participants
n=93 Participants
6 Participants
n=4 Participants
10 Participants
n=27 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
32 Participants
n=93 Participants
30 Participants
n=4 Participants
62 Participants
n=27 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=93 Participants
0 Participants
n=4 Participants
0 Participants
n=27 Participants
Region of Enrollment
United States
36 participants
n=93 Participants
36 participants
n=4 Participants
72 participants
n=27 Participants
Body Weight
78.93 kg
STANDARD_DEVIATION 11.472 • n=93 Participants
79.39 kg
STANDARD_DEVIATION 11.057 • n=4 Participants
79.16 kg
STANDARD_DEVIATION 11.189 • n=27 Participants

PRIMARY outcome

Timeframe: Days 1 to 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles. All completers had evaluable PK.

Cmax was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). Cmax was measured in micro grams per milliliter (µg/mL).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Maximum Observed Concentration (Cmax) of Single Dose Abatacept - Pharmacokinetic Evaluable Population
262 µg/mL
Geometric Coefficient of Variation 27
255 µg/mL
Geometric Coefficient of Variation 21

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

Tmax was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). Tmax was measured in hours (h).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Time to Reach Maximum Concentration (Tmax) of Single Dose Abatacept - Pharmacokinetic Evaluable Population
1.00 h
Interval 0.55 to 2.12
1.00 h
Interval 1.0 to 6.0

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

AUC (0 - 28) was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). AUC (0 - 28) was measured in micro grams\*hours per milliliter (µg\*h/mL).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Area Under the Concentration-time Curve (AUC) From Time Zero to 28 Days [AUC(0-28 Days)] of Single Dose Abatacept - Pharmacokinetic Evaluable Population
33195 µg*h/mL
Geometric Coefficient of Variation 26
35255 µg*h/mL
Geometric Coefficient of Variation 23

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

AUC (0 - T) was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). AUC (0 - T) was measured in micro grams\*hour per milliliter (µg\*h/mL).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Area Under the Concentration-time Curve From Zero to the Last Time of the Last Quantifiable Concentration [AUC(0-T)] of Single Dose Abatacept - Pharmacokinetic Evaluable Population
39295 µg*h/mL
Geometric Coefficient of Variation 23
41916 µg*h/mL
Geometric Coefficient of Variation 25

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

AUC (0 - INF) was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). AUC (0 - INF) was measured in µg\*h/mL.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Area Under the Concentration-time Curve From Time Zero Extrapolated to Infinity [AUC(0 - INF)] of Single Dose Abatacept - Pharmacokinetic Evaluable Population
40385 µg*h/mL
Geometric Coefficient of Variation 23
43229 µg*h/mL
Geometric Coefficient of Variation 27

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

T-HALF was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). T-HALF was measured in hours (h).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Terminal Phase Elimination Half-life (T-HALF) of Single Dose Abatacept - Pharmacokinetic Evaluable Population
344 h
Standard Deviation 87.6
364 h
Standard Deviation 106

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

CLT was the volume of abatacept cleared by the system, normalized by baseline body weight. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). CLT was measured in milliliters per hours per kilogram of body weight (mL/h/kg).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Total Body Clearance (CLT) of Single Dose Abatacept - Pharmacokinetic Evaluable Population
0.237 mL/h/kg
Geometric Coefficient of Variation 24
0.219 mL/h/kg
Geometric Coefficient of Variation 24

PRIMARY outcome

Timeframe: Day 1 to Day 71

Population: Pharmacokinetic (PK) population: all participants who received study drug and had adequate PK profiles.

Vss was derived from serum concentration versus time data. Serum samples were analyzed for abatacept by a validated enzyme-linked immunosorbent assay (ELISA) and were obtained at: predose (0 hours), 0.25 hours (h), 0.5, 1, 2, 6, 12, 24, 72, 168, 336, 504, 672, 1008, 1344, and 1688 h post dose (Days 1 to 71). The results were summarized. The lower limit of assay quantitation (LLOQ) was 1.00 nanograms per milliliter (ng/mL). Vss was measured in liters per kg body weight (L/kg).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=32 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=33 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Volume of Distribution at Steady-state (Vss) of Single Dose Abatacept - Pharmacokinetic Evaluable Population
0.088 L/kg
Geometric Coefficient of Variation 33
0.083 L/kg
Geometric Coefficient of Variation 22

SECONDARY outcome

Timeframe: Days 29, 57, 71

Population: Immunogenicity Data Set: All participants with at least 1 postdose visit.

Immunogenicity determination was based on titers of anti-abatacept and anti- cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4-T) antibodies in serum over time. A participant had a positive abatacept-induced immunogenicity if 1 of the following criteria were met: missing baseline measurement and a positive response after baseline; negative baseline response and positive response after baseline; a baseline response and a positive response after baseline that has a titer value strictly greater than the baseline titer value. A validated, sensitive, electrochemiluminescence assay (ECL) method was used to analyze the antibodies in serum. Samples confirmed positive with ECL and with abatacept serum concentrations of less than equal to 1 µg/mL were further analyzed with a validated, in vitro, cell-based bioassay to analyze the sera containing the abatacept neutralizing activity. Samples obtained on Days 29, 57 and 71 post dose of abatacept on Day 1 (baseline).

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Number of Participants With Positive Abatacept-induced Immunogenicity Response
4 participants
1 participants

SECONDARY outcome

Timeframe: Day 2 to Day 71

Population: All participants who received study drug (safety population) and had a laboratory assessment were included in the analysis. Day 2 N=36 both arms; Day 15 (Lonza arm N=36; Devens arm N=34; Day 29 (Lonza arm N=34; Devens arm N=33); Day 57 (Lonza arm N=31; Devens arm N=33); Day 71 (Lonza arm N=36; Devens arm N=34).

Blood samples obtained: Days 1, 2, 4, 8, 15, 22, 29, 43, 57 and 71. International Units per liter (U/L); milligram per deciliter (mg/dL); Male(M); Female (F). Reference ranges (low/high) for laboratories for which participants were identified with marked abnormalities during the study: Blood Urea Nitrogen (M/F) 10-20mg/dL ; Creatine Kinase (F) 21-21 U/L,(M) 32-294 U/L; Direct Bilirubin (M/F) 0.1-0.4 mg/dL ; Fasting Glucose (M/F) 70-110 mg/dL; Lactate Dehydrogenase (M/F) 110-209 U/L.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Creatine Kinase (high) Day 2 (N=36)
1 participants
0 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Creatine Kinase (high) Day 15 (N=36,34)
1 participants
0 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Creatine Kinase (high) Day 29 (N=34,33)
2 participants
1 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Creatine Kinase (high) Day 57 (N=31,33)
1 participants
1 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Creatine Kinase (high) Day 71 (N=36,34)
1 participants
2 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Lactate Dehydrogenase (high) Day 2 (N=36)
0 participants
1 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Blood urea nitrogen (high) Day 15 (N=36,34)
1 participants
0 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Blood urea nitrogen (high) Day 29 (N=34)
0 participants
1 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Blood urea nitrogen (high) Day 71 (N=36,34)
2 participants
0 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Bilirubin Direct (high) Day 29 (N=34)
0 participants
3 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Bilirubin Direct (high) Day 71 (N=36,34)
0 participants
1 participants
Number of Participants With Marked Serum Chemistry Abnormalities on Days 2, 15, 29, 57 and 71 - Safety Population
Fasting Glucose (high) Day 71 (N=36,34)
1 participants
0 participants

SECONDARY outcome

Timeframe: Day 2 to Day 72

Population: All participants who received study drug (safety population) and had a laboratory assessment were included in the analysis. Day 2 N=36 both arms; Day 15 (Lonza arm N=36; Devens arm N=34; Day 29 (Lonza arm N=34; Devens arm N=33); Day 57 (Lonza arm N=31; Devens arm N=33); Day 71 (Lonza arm N=36; Devens arm N=34).

Blood samples obtained: Days 2, 4, 8, 15, 22, 29, 43, 57 and 71. Male(M); Female (F). Reference ranges (low/high) for laboratory parameters for which participants were identified with marked abnormalities during the study: Leukocytes (quantitative White blood cells) (M/F) 4-11\*10\^3/microliters (µL); Neutrophils (absolute)(M/F) 1.4- 8.2\*10\^3/µL.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Any marked abnormalities Day 2 (N=36)
0 participants
0 participants
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Any marked abnormalities Day 15 (n=36,34)
0 participants
0 participants
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Leukocytes (high) Day 29 (N=34,33)
0 participants
1 participants
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Leukocytes (high) Day 57 (N=31,33)
0 participants
1 participants
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Leukocytes (high) Day 71 (N=36,34)
1 participants
1 participants
Number of Participants With Marked Hematology Abnormalities on Days 2, 15, 29, 57, and 71 - Safety Population
Neutrophils (absolute) (high) Day 71 (N=36,34)
1 participants
1 participants

SECONDARY outcome

Timeframe: Day 1 to Day 71

Population: All participants who received study drug (safety population) and had systolic assessment were included in the analysis. Days 1 and 2 N=36 both arms; Day 15 (Lonza arm N=36; Devens arm N=34; Day 29 (N=33 in both arms); Day 57 (Lonza arm N=31; Devens arm N=33); Day 71 (Lonza arm N=36; Devens arm N=34).

Blood pressure was obtained while the participant had been quietly seated for at least 5 minutes. Baseline was the 0 hour measurement on Day 1 (day of dosing) or if this value was missing, the last measurement before dosing. Blood pressure was measured in millimeters of mercury (mmHg) on Days 1, 2, 15, 29, 57, and 71.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 1 (1 hour post dose) (N=36)
-6.3 mmHg
Standard Deviation 9.73
-4.5 mmHg
Standard Deviation 9.06
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 2 (N=36)
-3.0 mmHg
Standard Deviation 12.31
-1.4 mmHg
Standard Deviation 10.35
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 15 (N=36,34)
-1.1 mmHg
Standard Deviation 12.09
0.3 mmHg
Standard Deviation 8.12
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 29 (N=33)
-2.3 mmHg
Standard Deviation 10.94
-0.2 mmHg
Standard Deviation 9.65
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 57 (N=31,33)
1.0 mmHg
Standard Deviation 11.96
3.4 mmHg
Standard Deviation 9.48
Change From Baseline in Systolic Blood Pressure - Safety Population
Day 71 (N=36,34)
2.2 mmHg
Standard Deviation 12.09
2.9 mmHg
Standard Deviation 9.87

SECONDARY outcome

Timeframe: Day 1 to Day 71

Population: All participants who received study drug (safety population) and had diastolic assessment were included in the analysis. Day 2 N=36 both arms; Day 15 (Lonza arm N=36; Devens arm N=34; Day 29 (N=33 in both arms); Day 57 (Lonza arm N=31; Devens arm N=33); Day 71 (Lonza arm N=36; Devens arm N=34).

Blood pressure was obtained while the participant had been quietly seated for at least 5 minutes. Baseline was the 0 hour measurement on Day 1 (day of dosing) or if this value was missing, the last measurement before dosing. Blood pressure was measured in millimeters of mercury (mmHg) on Days 1, 2, 15, 29, 57, and 71.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 2 (N=36)
-2.3 mmHg
Standard Deviation 8.98
-0.6 mmHg
Standard Deviation 6.02
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 1 (1 hour post dose) N=36
-4.9 mmHg
Standard Deviation 5.91
-4.1 mmHg
Standard Deviation 5.78
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 15 (N=36,34)
-1.4 mmHg
Standard Deviation 7.45
1.0 mmHg
Standard Deviation 7.29
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 29 (N=33)
-1.9 mmHg
Standard Deviation 6.37
0.9 mmHg
Standard Deviation 7.08
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 57 (N=31,33)
0.4 mmHg
Standard Deviation 7.48
3.5 mmHg
Standard Deviation 7.56
Change From Baseline in Diastolic Blood Pressure on Days 1, 2, 15, 29, 57, and 71 - Safety Population
Day 71 (N=36,34)
1.6 mmHg
Standard Deviation 8.59
4.0 mmHg
Standard Deviation 5.78

SECONDARY outcome

Timeframe: Day 1 to Day 71

Population: All participants who received study drug and had at least one ECG value.

12-lead electrocardiograms were performed on a supine participant (5 minutes supine) at baseline (baseline = screening; Days -21 to -2) and at Day 71. QT interval and QTc were measured in mille seconds (msec). A change from baseline QT and QTc (corrected for heart rate by Fridericia formula) greater than (\>) 30 msec or less than (\<) 60 msec were presented, as well as values over 450 and 500 msec. QT interval on ECG image defined as: time from the beginning of the QRS (complex consisting of Q, R and S waves) to the end of the T wave.

Outcome measures

Outcome measures
Measure
750 mg Abatacept From Lonza, NH
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Devens, MA
n=36 Participants
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
Change from baseline in QT >60 msec
0 participants
1 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
Change from baseline in QT >30 msec < 60 msec
5 participants
4 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
Change from baseline in QTc >60 msec
0 participants
0 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
Change from baseline in QTc >30 msec < 60 msec
2 participants
2 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
QT > 500 msec
0 participants
0 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
QT > 450 msec
0 participants
1 participants
Number of Participants With a Change From Baseline in QT Interval and Corrected (Fridericia) QT Interval (QTcF) - Safety Population
QTcF >450 msec
0 participants
0 participants

Adverse Events

750 mg Abatacept From Devens, MA

Serious events: 0 serious events
Other events: 11 other events
Deaths: 0 deaths

750 mg Abatacept From Lonza, NH

Serious events: 0 serious events
Other events: 12 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
750 mg Abatacept From Devens, MA
n=36 participants at risk
A single dose of abatacept 750 mg, manufactured at Devens, Massachusetts facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
750 mg Abatacept From Lonza, NH
n=36 participants at risk
A single dose of abatacept 750 mg, manufactured at Lonza, New Hampshire facility, was administered intravenously (IV) using a calibrated, constant rate infusion pump over approximately 30 minutes.
Gastrointestinal disorders
Diarrhoea
5.6%
2/36 • Day 1 to Day 71
2.8%
1/36 • Day 1 to Day 71
Nervous system disorders
Dizziness
5.6%
2/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
8.3%
3/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71
Musculoskeletal and connective tissue disorders
Back pain
0.00%
0/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71
Eye disorders
Vision blurred
0.00%
0/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71
Respiratory, thoracic and mediastinal disorders
Cough
5.6%
2/36 • Day 1 to Day 71
11.1%
4/36 • Day 1 to Day 71
Respiratory, thoracic and mediastinal disorders
Nasal congestion
8.3%
3/36 • Day 1 to Day 71
0.00%
0/36 • Day 1 to Day 71
Infections and infestations
Upper respiratory tract infection
2.8%
1/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71
Nervous system disorders
Headache
13.9%
5/36 • Day 1 to Day 71
16.7%
6/36 • Day 1 to Day 71
Respiratory, thoracic and mediastinal disorders
Rhinorrhoea
0.00%
0/36 • Day 1 to Day 71
8.3%
3/36 • Day 1 to Day 71
Renal and urinary disorders
Dysuria
0.00%
0/36 • Day 1 to Day 71
5.6%
2/36 • Day 1 to Day 71

Additional Information

Bristol-Myers Squibb Study Director

Bristol-Myers Squibb

Results disclosure agreements

  • Principal investigator is a sponsor employee Bristol-Myers Squibb Co. agreements with investigators vary; constant is our right to embargo communications regarding trial results prior to public release for a period ≤60 days from submittal for review. We will not prohibit investigators from publishing, but will prohibit the disclosure of previously undisclosed confidential information other than study results, and request postponement of single-center publications until after disclosure of the clinical trial's primary publication.
  • Publication restrictions are in place

Restriction type: OTHER