Trial Outcomes & Findings for Safety and Immunogenicity of a Human Hookworm Candidate Vaccine With Different Doses of a Novel Adjuvant (NCT NCT01385189)

NCT ID: NCT01385189

Last Updated: 2025-01-06

Results Overview

Frequency of vaccine-related AEs, graded by severity, for each dose and formulation of Na-GST-1

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

40 participants

Primary outcome timeframe

2 hours post vaccination

Results posted on

2025-01-06

Participant Flow

Participant milestones

Participant milestones
Measure
Cohort 1
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Overall Study
STARTED
12
12
4
4
8
Overall Study
COMPLETED
11
11
4
3
6
Overall Study
NOT COMPLETED
1
1
0
1
2

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Safety and Immunogenicity of a Human Hookworm Candidate Vaccine With Different Doses of a Novel Adjuvant

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Cohort 1
n=12 Participants
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
n=12 Participants
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
n=4 Participants
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
n=4 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
n=8 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Total
n=40 Participants
Total of all reporting groups
Age, Continuous
34.3 years
n=5 Participants
33.8 years
n=7 Participants
26.3 years
n=5 Participants
23.3 years
n=4 Participants
35.0 years
n=21 Participants
32.9 years
n=8 Participants
Sex: Female, Male
Female
4 Participants
n=5 Participants
5 Participants
n=7 Participants
1 Participants
n=5 Participants
1 Participants
n=4 Participants
5 Participants
n=21 Participants
16 Participants
n=8 Participants
Sex: Female, Male
Male
8 Participants
n=5 Participants
7 Participants
n=7 Participants
3 Participants
n=5 Participants
3 Participants
n=4 Participants
3 Participants
n=21 Participants
24 Participants
n=8 Participants

PRIMARY outcome

Timeframe: 2 hours post vaccination

Frequency of vaccine-related AEs, graded by severity, for each dose and formulation of Na-GST-1

Outcome measures

Outcome measures
Measure
Cohort 1
n=12 Participants
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
n=12 Participants
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
n=4 Participants
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
n=4 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
n=8 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
100 µg Na-GST-1/Alhydrogel
100 µg Na-GST-1/Alhydrogel/GLA-AF (5 µg)
Immediate Vaccine Related Adverse Events
4 Participants
1 Participants
0 Participants
1 Participants
4 Participants

SECONDARY outcome

Timeframe: 126 days post dose 1

Dose and formulation of Na-GST-1 that generates the highest IgG antibody response at Day 126, as determined by an indirect enzyme-linked immunosorbent assay (ELISA)

Outcome measures

Outcome measures
Measure
Cohort 1
n=4 Participants
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
n=7 Participants
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
n=3 Participants
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
n=7 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
n=4 Participants
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
100 µg Na-GST-1/Alhydrogel
n=3 Participants
100 µg Na-GST-1/Alhydrogel/GLA-AF (5 µg)
n=6 Participants
IgG Antibody Response to Na-GST-1
17.12 Arbitrary Units
Standard Deviation 24.11
210.02 Arbitrary Units
Standard Deviation 366.67
66.86 Arbitrary Units
Standard Deviation 105.60
64.65 Arbitrary Units
Standard Deviation 74.86
69.34 Arbitrary Units
Standard Deviation 107.46
117.24 Arbitrary Units
Standard Deviation 117.99
89.42 Arbitrary Units
Standard Deviation 97.80

Adverse Events

Cohort 1

Serious events: 0 serious events
Other events: 12 other events
Deaths: 0 deaths

Cohort 2

Serious events: 0 serious events
Other events: 12 other events
Deaths: 0 deaths

Cohort 3

Serious events: 0 serious events
Other events: 4 other events
Deaths: 0 deaths

Cohort 4

Serious events: 0 serious events
Other events: 4 other events
Deaths: 0 deaths

Cohort 5

Serious events: 1 serious events
Other events: 8 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Cohort 1
n=12 participants at risk
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
n=12 participants at risk
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
n=4 participants at risk
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
n=4 participants at risk
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
n=8 participants at risk
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Psychiatric disorders
Delusional Episode
0.00%
0/12
0.00%
0/12
0.00%
0/4
0.00%
0/4
12.5%
1/8 • Number of events 1

Other adverse events

Other adverse events
Measure
Cohort 1
n=12 participants at risk
10 μg Na-GST-1/Alhydrogel vs. 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 10 μg Na-GST-1/Alhydrogel: 3 doses 10 μg Na-GST-1/Alhydrogel administered at 56 day intervals 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses 10 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 2
n=12 participants at risk
30 μg Na-GST-1/Alhydrogel vs. 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 30 μg Na-GST-1/Alhydrogel: 3 doses 30 μg Na-GST-1/Alhydrogel administered at 56 day intervals 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg): 3 doses of 30 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) administered at 56 day intervals
Cohort 3
n=4 participants at risk
30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 30 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
Cohort 4
n=4 participants at risk
100 μg Na-GST-1/Alhydrogel/GLA-AF (1 μg) 100 μg Na-GST-1/Alhydrogel: 3 doses 100 μg Na-GST-1/Alhydrogel administered at 56 day intervals
Cohort 5
n=8 participants at risk
100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg): 3 doses 100 μg Na-GST-1/Alhydrogel/GLA-AF (5 μg) administered at 56 day intervals
General disorders
Injection Site Pain
50.0%
6/12 • Number of events 13
33.3%
4/12 • Number of events 8
75.0%
3/4 • Number of events 5
25.0%
1/4 • Number of events 3
62.5%
5/8 • Number of events 8
General disorders
Injection Site Tenderness
33.3%
4/12 • Number of events 9
41.7%
5/12 • Number of events 9
75.0%
3/4 • Number of events 6
50.0%
2/4 • Number of events 2
50.0%
4/8 • Number of events 8
General disorders
Injection Site Erythema
0.00%
0/12
0.00%
0/12
0.00%
0/4
0.00%
0/4
25.0%
2/8 • Number of events 2
General disorders
Injection Site Swelling
8.3%
1/12 • Number of events 1
0.00%
0/12
25.0%
1/4 • Number of events 1
50.0%
2/4 • Number of events 2
25.0%
2/8 • Number of events 2
Gastrointestinal disorders
Nausea
25.0%
3/12 • Number of events 4
16.7%
2/12 • Number of events 2
25.0%
1/4 • Number of events 1
0.00%
0/4
25.0%
2/8 • Number of events 2
Nervous system disorders
Headache
66.7%
8/12 • Number of events 11
58.3%
7/12 • Number of events 10
50.0%
2/4 • Number of events 4
25.0%
1/4 • Number of events 1
12.5%
1/8 • Number of events 1
Gastrointestinal disorders
Vomiting
8.3%
1/12 • Number of events 2
8.3%
1/12 • Number of events 1
0.00%
0/4
25.0%
1/4 • Number of events 1
12.5%
1/8 • Number of events 2
Musculoskeletal and connective tissue disorders
Myalgia
8.3%
1/12 • Number of events 1
16.7%
2/12 • Number of events 2
0.00%
0/4
0.00%
0/4
0.00%
0/8
Musculoskeletal and connective tissue disorders
Arthrlagia
0.00%
0/12
8.3%
1/12 • Number of events 1
0.00%
0/4
0.00%
0/4
0.00%
0/8
Immune system disorders
Urticaria
0.00%
0/12
0.00%
0/12
0.00%
0/4
25.0%
1/4 • Number of events 1
0.00%
0/8

Additional Information

Dr. David J. Diemert, Associate Professor

George Washington University

Phone: 202.994.2909

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place