Trial Outcomes & Findings for Biodistribution and Safety of the PET Probes [18F]FPRGD2 and [18F]FPPRGD2 (NCT NCT01383135)

NCT ID: NCT01383135

Last Updated: 2024-01-12

Results Overview

Normal radiopharmaceutical biodistribution was analyzed visually to obtain dosimetry data in healthy volunteers. Organs with the highest radiation absorbed dose (dosimetry) are provided below. Dosimetry was calculated by drawing regions-of-interest around organs with visually appreciable radiopharmaceutical uptake greater than background and using organ-level internal dose assessment software from Vanderbuilt University (2003). Results are reported in mSv/MBq (milli-Sieverts per mega-Bequerel) which is a measurement of the mean absorbed radiation dose within an organ.

Recruitment status

COMPLETED

Study phase

EARLY_PHASE1

Target enrollment

27 participants

Primary outcome timeframe

5 hours

Results posted on

2024-01-12

Participant Flow

Participant milestones

Participant milestones
Measure
Healthy Volunteers
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection.
Breast Cancer
4 to 11 mCi F18-FPPRGD2 by IV injection
Glioblastoma Multiform Patients
5 to14 mCi F18-FPPRGD2 by IV injection
Lung Cancer
X to XX mCi F18-FPPRGD2 by IV injection
Overall Study
STARTED
6
8
9
4
Overall Study
COMPLETED
5
8
8
4
Overall Study
NOT COMPLETED
1
0
1
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Healthy Volunteers
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection.
Breast Cancer
4 to 11 mCi F18-FPPRGD2 by IV injection
Glioblastoma Multiform Patients
5 to14 mCi F18-FPPRGD2 by IV injection
Lung Cancer
X to XX mCi F18-FPPRGD2 by IV injection
Overall Study
Not Scanned / Imaged
1
0
1
0

Baseline Characteristics

Biodistribution and Safety of the PET Probes [18F]FPRGD2 and [18F]FPPRGD2

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Healthy Volunteers
n=6 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Breast Cancer Patients
n=8 Participants
4 to 11 mCi F18-FPPRGD2 by IV injection
Glioblastoma Multiforme
n=9 Participants
5 to14 mCi F18-FPPRGD2 by IV injection
Lung Cancer
n=4 Participants
X to XX mCi F18-FPPRGD2 by IV injection
Total
n=27 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Age, Categorical
Between 18 and 65 years
6 Participants
n=5 Participants
6 Participants
n=7 Participants
7 Participants
n=5 Participants
3 Participants
n=4 Participants
22 Participants
n=21 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
2 Participants
n=7 Participants
2 Participants
n=5 Participants
1 Participants
n=4 Participants
5 Participants
n=21 Participants
Sex: Female, Male
Female
4 Participants
n=5 Participants
8 Participants
n=7 Participants
4 Participants
n=5 Participants
2 Participants
n=4 Participants
18 Participants
n=21 Participants
Sex: Female, Male
Male
2 Participants
n=5 Participants
0 Participants
n=7 Participants
5 Participants
n=5 Participants
2 Participants
n=4 Participants
9 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
2 Participants
n=5 Participants
7 Participants
n=7 Participants
8 Participants
n=5 Participants
2 Participants
n=4 Participants
19 Participants
n=21 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
4 Participants
n=5 Participants
1 Participants
n=7 Participants
1 Participants
n=5 Participants
2 Participants
n=4 Participants
8 Participants
n=21 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=7 Participants
4 Participants
n=5 Participants
0 Participants
n=4 Participants
4 Participants
n=21 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Race (NIH/OMB)
Black or African American
0 Participants
n=5 Participants
1 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
1 Participants
n=21 Participants
Race (NIH/OMB)
White
5 Participants
n=5 Participants
6 Participants
n=7 Participants
3 Participants
n=5 Participants
2 Participants
n=4 Participants
16 Participants
n=21 Participants
Race (NIH/OMB)
More than one race
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
0 Participants
n=21 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=5 Participants
1 Participants
n=7 Participants
2 Participants
n=5 Participants
2 Participants
n=4 Participants
6 Participants
n=21 Participants

PRIMARY outcome

Timeframe: 5 hours

Population: Per protocol, data were collected for this outcome from the Healthy Volunteers group only, and only those that completed all scans are included.

Normal radiopharmaceutical biodistribution was analyzed visually to obtain dosimetry data in healthy volunteers. Organs with the highest radiation absorbed dose (dosimetry) are provided below. Dosimetry was calculated by drawing regions-of-interest around organs with visually appreciable radiopharmaceutical uptake greater than background and using organ-level internal dose assessment software from Vanderbuilt University (2003). Results are reported in mSv/MBq (milli-Sieverts per mega-Bequerel) which is a measurement of the mean absorbed radiation dose within an organ.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=4 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Tracer Dosimetry by Organ
Kidneys
0.3603 mSv/MBq
Standard Deviation 0.1852
Tracer Dosimetry by Organ
Bladder
0.8615 mSv/MBq
Standard Deviation 0.4362
Tracer Dosimetry by Organ
Bowel
0.5290 mSv/MBq
Standard Deviation 0.2364
Tracer Dosimetry by Organ
Liver
0.0988 mSv/MBq
Standard Deviation 0.0379

PRIMARY outcome

Timeframe: 30, 60, and 90 minutes post-injection

Population: Per protocol, data were collected for this outcome from the Healthy Volunteers group only. 4/5 volunteers were able to complete all of the scans. 1/5 participant could only complete one of the four scans and was not included in pharmacokinetic calculations.

Radiopharmaceutical pharmacokinetics describe the change in radiopharmaceutical distribution in the body (from the blood to organs, tissues, cells) over time. Radiopharmaceutical pharmacokinetics are used to determine optimal imaging time, ie, when target activity (organ or cell of interest) is greater than background activity (blood). Optimal imaging time must also be balanced with tracer bio-metabolism and radioactive decay. F18-FPPRGD2 pharmacokinetics was measured in healthy volunteers to estimate optimal imaging time. Scans were used to visually identify regions of interest (organs of F18-FPPRGD2 accumulation), and detected radiation was plotted as a measurement over time.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=4 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
F18-FPPRGD2 Time-activity at Specified Timepoints
30 minutes post-injection
26 Percentage of tracer remaining in blood
Standard Deviation 17
F18-FPPRGD2 Time-activity at Specified Timepoints
60 minutes post-injection
18 Percentage of tracer remaining in blood
Standard Deviation 14
F18-FPPRGD2 Time-activity at Specified Timepoints
90 minutes post-injection
8 Percentage of tracer remaining in blood
Standard Deviation 2

PRIMARY outcome

Timeframe: 3 hours

Population: Per protocol, data were collected for this outcome from the Breast Cancer Patients group only. In a per-lesion analysis, a total of 30 lesions were evaluated at PET/CT scanning with F18-FPPRGD2.

Sensitivity is the ability of a test to correctly identify patients with the disease being investigated. In this instance, how well F18-FPPRGD2 PET/CT detects true-positive patients. Sensitivity is defined as \[TP/ (TP+FN)\], where TP= true positive, and FN = false negative. The outcome is reported as a percentage without dispersion. A higher percentage indicates a greater probability that a lesion identified based on scan results is cancerous, and a lower percentage indicates reduced confidence in that result.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=30 Lesions
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Sensitivity of F18-FPPRGD2 PET/CT in Breast Cancer
95.7 percentage of sensitivity

PRIMARY outcome

Timeframe: an estimated average of 3 hours

Population: Per protocol, data were collected for this outcome from the Breast Cancer Patients group only. Other cohorts are not included, and the outcome was only assessed in the breast cancer cohort.

Specificity is the ability of a test to correctly identify patients who do not have the disease being investigated. In this instance, how well F18 FPPRGD2 PET/CT detects true-negative patients. Specificity is: \[TN/ (TN+FP)\], where TN= true negative, and FP = false positive.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=8 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Specificity of F18 FPPRGD2 PET/CT in Breast Cancer
100 percentage of specificity

PRIMARY outcome

Timeframe: Baseline and Week 6

Population: Per protocol, data were collected for this outcome from the Glioblastoma Multiforme group only. Participants in the Glioblastoma Multiforme group who completed the imaging schedule were included in the analysis.

Primary tumor response was assessed after 6 weeks of treatment as the change in tumor metabolism based on the maximum standardized uptake value (SUVmax) as determined by positron emission tomography (PET) scans. Decreased SUVmax correlates to a reduction of tumor metabolism, and is considered an indicator of primary tumor response. Reduction of SUVmax was determined as the change from baseline in uptake of F-18FPPRGD2. The outcome is reported as the baseline and week 6 values, with standard deviation.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=6 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Glioblastoma Primary Tumor Response Assessed by PET Scan
Pretreatment
1.7 SUVmax
Standard Deviation 0.6
Glioblastoma Primary Tumor Response Assessed by PET Scan
Week 6
1.3 SUVmax
Standard Deviation 0.5

SECONDARY outcome

Timeframe: Baseline and Week 6

Population: Per protocol, data were collected for this outcome from the Glioblastoma Multiforme group only. Participants in the Glioblastoma Multiforme group who completed the imaging schedule were included in the analysis.

Primary tumor response was assessed after 6 weeks of treatment as the change in tumor metabolism based on the maximum standardized uptake value (SUVmax) as determined by computed tomography (CT) scans. Decreased SUVmax correlates to a reduction of tumor metabolism, and is considered an indicator of primary tumor response. Reduction of SUVmax was determined as the change from baseline in uptake of F-18FPPRGD2. The outcome is reported as the mean difference from baseline to week 6, with standard deviation.

Outcome measures

Outcome measures
Measure
Healthy Volunteers
n=6 Participants
5 to 14 mCi F18-FPPRGD2 by intravenous (IV) injection
Glioblastoma Primary Tumor Response Assessed by CT Scan
-11.0 percentage change
Standard Deviation 80.7

Adverse Events

Healthy Volunteers

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Breast Cancer Patients

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Glioblastoma Multiforme

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Lung Cancer

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Andrei Iagaru, MD

Stanford University

Phone: 6507362859

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place