Safety and Tolerability of FITC-Adalimumab Administration During Confocal Laser Endomicroscopy of the Gut

NCT ID: NCT01275508

Last Updated: 2012-09-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

25 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-01-31

Study Completion Date

2012-04-30

Brief Summary

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The scientific objective of the project is to evaluate if the topical administration of Fluoresceinisothiocyanate (FITC)-Adalimumab during the endomicroscopic examination of the gut in Crohn's disease (CD) patients shows an acceptable safety profile. Another objective is to explore whether the use of FITC-Adalimumab as a predictive biomarker might reliably predict patient response to Adalimumab therapy and hence enable optimized utilization of this treatment option.

Detailed Description

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Conditions

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Crohn's Disease

Keywords

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Subjects with active Crohn's disease Safety and tolerability of FITC-Adalimumab Intestinal topical application

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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FITC-Adalimumab

Group Type EXPERIMENTAL

FITC-Adalimumab

Intervention Type DRUG

The study product FITC-Adalimumab will be topically applied via a spray catheter to the intestinal mucosa of Crohn's disease patients during endomicroscopy of the gut. One mL of the diluted study product will be applied each time to overall four selected intestinal areas at a concentration of 20 μg/mL respectively (equivalent to 20 μg of FITC-Adalimumab per area). Total amount applied will be 80 μg FITC-Adalimumab per patient.

Interventions

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FITC-Adalimumab

The study product FITC-Adalimumab will be topically applied via a spray catheter to the intestinal mucosa of Crohn's disease patients during endomicroscopy of the gut. One mL of the diluted study product will be applied each time to overall four selected intestinal areas at a concentration of 20 μg/mL respectively (equivalent to 20 μg of FITC-Adalimumab per area). Total amount applied will be 80 μg FITC-Adalimumab per patient.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Male and female subjects from 18 to 70 years of age who are capable of giving informed consent without any restrictions
* Endoscopically and histologically confirmed diagnosis of Crohn's disease (CD)
* Manifestation of CD in the colon and/or in the terminal ileum
* Currently active CD with a CDAI score \>150
* Clinically inadequate response of CD to systemic glucocorticoids and/or immunosuppressants (azathioprine, 6-mercaptopurine, methotrexate) or glucocorticoid-dependent CD or any contraindication to glucocorticoids
* Indication for treatment with Adalimumab
* Indication for intestinal confocal laser endomicroscopy prior to institution of adalimumab therapy
* Subject is mentally capable of understanding the nature and purpose/conduct of the clinical trial and of following the study staff's instructions
* Subject has given written informed consent after being informed by an investigator

Female subjects additionally must meet at least one of the following criteria:

* Must be menopausal (at least 12 months' natural amenorrhea or 6 months' amenorrhea with serum FSH \>40 mU/mL) or
* Must have undergone bilateral oophorectomy or hysterectomy or
* Must be a regular, correct, and reliable user of a contraceptive method with a failure rate of \<1% per year (such as oral contraceptives, implants, depot shots, intrauterine device, hormone coil)
* Must have a vasectomized partner

Exclusion Criteria

* Impaired blood clotting (prothrombin rate \<50% and/or PTT \>55 sec and/or a platelet count of \<50,000/μL)
* Pregnancy and lactation

Contraindications to treatment with adalimumab:

* Moderate to severe heart failure (NYHA Class III/IV)
* Active tuberculosis
* Severe acute infections, e.g. sepsis
* Opportunistic infections including invasive fungal infections

* Known hypersensitivity to any of the ingredients of the study product or to any drug with a similar chemical structure
* Treatment with beta-receptor blocking agents (because of altered symptoms in case of possible anaphylactic reactions to IV fluorescein)
* Any other condition or medical treatment that, in the investigator's judgment, is not compatible with participation in the trial
* Individuals who are in a relationship of dependence on or employed by the sponsor or any of the investigators
* Planned prolonged stay outside the region of the study site, preventing the subject from returning for scheduled visits
* Participation in any other clinical trial or administration of any investigational drug within the last four weeks prior to the screening visit
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Abbott

INDUSTRY

Sponsor Role collaborator

University of Erlangen-Nürnberg Medical School

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Markus Neurath, MD PhD

Role: STUDY_DIRECTOR

Medizinische Klinik I, University Hospital Erlangen

Raja Atreya, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Medizinische Klinik I, University Hospital Erlangen

Locations

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Medizinische Klinik I, University Hospital Erlangen

Erlangen, Bavaria, Germany

Site Status

Countries

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Germany

References

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Colombel JF, Sandborn WJ, Rutgeerts P, Enns R, Hanauer SB, Panaccione R, Schreiber S, Byczkowski D, Li J, Kent JD, Pollack PF. Adalimumab for maintenance of clinical response and remission in patients with Crohn's disease: the CHARM trial. Gastroenterology. 2007 Jan;132(1):52-65. doi: 10.1053/j.gastro.2006.11.041. Epub 2006 Nov 29.

Reference Type BACKGROUND
PMID: 17241859 (View on PubMed)

Keller R, Winde G, Terpe HJ, Foerster EC, Domschke W. Fluorescence endoscopy using a fluorescein-labeled monoclonal antibody against carcinoembryonic antigen in patients with colorectal carcinoma and adenoma. Endoscopy. 2002 Oct;34(10):801-7. doi: 10.1055/s-2002-34254.

Reference Type BACKGROUND
PMID: 12244502 (View on PubMed)

Kiesslich R, Burg J, Vieth M, Gnaendiger J, Enders M, Delaney P, Polglase A, McLaren W, Janell D, Thomas S, Nafe B, Galle PR, Neurath MF. Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo. Gastroenterology. 2004 Sep;127(3):706-13. doi: 10.1053/j.gastro.2004.06.050.

Reference Type BACKGROUND
PMID: 15362025 (View on PubMed)

Kiesslich R, Goetz M, Vieth M, Galle PR, Neurath MF. Technology insight: confocal laser endoscopy for in vivo diagnosis of colorectal cancer. Nat Clin Pract Oncol. 2007 Aug;4(8):480-90. doi: 10.1038/ncponc0881.

Reference Type BACKGROUND
PMID: 17657253 (View on PubMed)

Other Identifiers

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2010-018959-99

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

FITC-ADA 01-2010

Identifier Type: -

Identifier Source: org_study_id