Differences in Evoked Intracellular Interferon Signaling Pathways Using Single Cell Phosphoprotein Profiling

NCT ID: NCT01217359

Last Updated: 2016-11-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

22 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-05-31

Study Completion Date

2012-08-31

Brief Summary

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Purpose:

The purpose of this exploratory study is to evaluate phosphoprotein profiling to determine differences in the intracellular actions of interferon or interferon stimulated genes among people with different treatment outcomes to interferon based therapy for hepatitis C (HCV). Participants: Patients with genotype 1 HCV who have had interferon based therapy at the University of North Carolina (UNC). Procedures: Thirty patients with varied responses to treatment will be given a single subcutaneous injection of interferon alpha 2b. Prior to the injection and at 30 minutes, 1,2,4,6,12 and 20 hours after injection, blood will be drawn for analysis of phosphoprotein profiling and changes in serum cytokines.

Detailed Description

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Conditions

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Hepatitis C

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Blinding Strategy

NONE

Study Groups

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Interferon Alfa-2b

Patients will receive a single dose of interferon

Group Type EXPERIMENTAL

Interferon Alfa-2b

Intervention Type DRUG

Patients will receive a single dose of interferon followed by serial blood draws to assess cellular responses to interferon

Interventions

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Interferon Alfa-2b

Patients will receive a single dose of interferon followed by serial blood draws to assess cellular responses to interferon

Intervention Type DRUG

Other Intervention Names

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Intron A

Eligibility Criteria

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Inclusion Criteria

* Willing and able to provide informed consent
* Males or females age 18 - 65 years
* For women of childbearing potential, negative blood pregnancy test documented within the 24-hour period prior to IFN-α administration
* History of genotype 1 HCV infection previously treated with peginterferon and ribavirin and with a virological response that can be determined from retrospective review of medical records
* A washout period of \>12 weeks between the last dose of prescribed peginterferon and ribavirin and the screening visit

Exclusion Criteria

Subjects with any of the following will not be eligible for participation:

* Neutrophil count \<1500 cells/mm3, Hgb \<12 g/dL in women or 13 g/dL in men, or platelet count \<75,000 cells/mm3 at screening
* Serum creatinine level \>1.5 times the upper limit of normal at screening
* Poorly controlled psychiatric illness, as determined by the study physician, which can be exacerbated by interferon therapy
* Infection requiring antibiotics, symptomatic viral infection, inflammatory process requiring therapy, or allergic reactions in the week immediately preceding the inpatient study visit
* Use of medications that affect the immune system (e.g. glucocorticoids, chronic NSAIDs daily) in the week immediately preceding the inpatient study visit
* History of immunologically mediated disease (e.g., inflammatory bowel disease, idiopathic thrombocytopenic purpura, lupus erythematosus, autoimmune hemolytic anemia, psoriasis, rheumatoid arthritis)
* Women with ongoing pregnancy or breast-feeding
* History or other evidence of bleeding from esophageal varices or other conditions consistent with decompensated liver disease
* History of a seizure disorder or current anticonvulsant use
* History of major organ transplantation with an existing functional graft
* History of thyroid disease poorly controlled on prescribed medications
* History or other evidence of severe illness or any other conditions which would make the patient, in the opinion of the investigator, unsuitable for the study
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Tibotec Pharmaceutical Limited

INDUSTRY

Sponsor Role collaborator

University of North Carolina, Chapel Hill

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Sid Barritt, MD, MSCR

Role: PRINCIPAL_INVESTIGATOR

University of North Carolina, Chapel Hill

Locations

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University of North Carolina

Chapel Hill, North Carolina, United States

Site Status

Countries

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United States

Other Identifiers

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08-2040

Identifier Type: -

Identifier Source: org_study_id