Trial Outcomes & Findings for Effects of Pioglitazone on High-density Lipoprotein (HDL) Function in Persons With Diabetes (NCT NCT01156597)

NCT ID: NCT01156597

Last Updated: 2014-11-21

Results Overview

The primary endpoint will be increased high density lipoprotein cholesterol and decreased triglycerides measured as the difference after 12 or 24 weeks of treatment from baseline levels. The data are expressed as the percent change from the baseline value and calculated using he equation: * Change=\[100%\*(Endpoint value - Baseline Value)/Baseline Value\]

Recruitment status

COMPLETED

Study phase

PHASE3

Target enrollment

30 participants

Primary outcome timeframe

24 weeks

Results posted on

2014-11-21

Participant Flow

Participant milestones

Participant milestones
Measure
Pioglitazone Group
This is a baseline versus treatment study comparing subjects on pioglitazone to a matched group of subjects treated with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level pioglitazone: 30 mg daily for three weeks increase to 45 mg daily for 21 more weeks
Comparator Group
This group of subjects will be maintained on standard treatment with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level as group treated with pioglitazone.
Overall Study
STARTED
16
14
Overall Study
COMPLETED
13
13
Overall Study
NOT COMPLETED
3
1

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Effects of Pioglitazone on High-density Lipoprotein (HDL) Function in Persons With Diabetes

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Pioglitazone Group
n=16 Participants
This is a baseline versus treatment study comparing subjects on pioglitazone to a matched group of subjects treated with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level The pioglitazone treatment regimen for this arm of the study: 30 mg daily for three weeks increase to 45 mg daily for 21 more weeks
Comparator Group
n=14 Participants
This group of subjects will be maintained on standard treatment with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level as group treated with pioglitazone.
Total
n=30 Participants
Total of all reporting groups
Age, Continuous
58.4 years
STANDARD_DEVIATION 5.9 • n=5 Participants
58.2 years
STANDARD_DEVIATION 8.2 • n=7 Participants
58.3 years
STANDARD_DEVIATION 5.9 • n=5 Participants
Sex: Female, Male
Female
6 Participants
n=5 Participants
2 Participants
n=7 Participants
8 Participants
n=5 Participants
Sex: Female, Male
Male
10 Participants
n=5 Participants
12 Participants
n=7 Participants
22 Participants
n=5 Participants
Region of Enrollment
United States
16 participants
n=5 Participants
14 participants
n=7 Participants
30 participants
n=5 Participants
Hemoglobin A1C
7.2 %
STANDARD_DEVIATION 1.3 • n=5 Participants
7.8 %
STANDARD_DEVIATION 1.3 • n=7 Participants
7.5 %
STANDARD_DEVIATION 1.2 • n=5 Participants
High Density Lipoprotein
45.3 mg/dl
STANDARD_DEVIATION 11.2 • n=5 Participants
48.0 mg/dl
STANDARD_DEVIATION 8.4 • n=7 Participants
46.7 mg/dl
STANDARD_DEVIATION 9.8 • n=5 Participants

PRIMARY outcome

Timeframe: 24 weeks

Population: All subjects that completed the study were used for the final analysis

The primary endpoint will be increased high density lipoprotein cholesterol and decreased triglycerides measured as the difference after 12 or 24 weeks of treatment from baseline levels. The data are expressed as the percent change from the baseline value and calculated using he equation: * Change=\[100%\*(Endpoint value - Baseline Value)/Baseline Value\]

Outcome measures

Outcome measures
Measure
Pioglitazone Group
n=13 Participants
This is a baseline versus treatment study comparing subjects on pioglitazone to a matched group of subjects treated with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level The pioglitazone treatment regimen for this arm of the study: 30 mg daily for three weeks increase to 45 mg daily for 21 more weeks
Comparator Group
n=13 Participants
This group of subjects will be maintained on standard treatment with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level as group treated with pioglitazone.
Increased HDL-Cholesterol and Decreased Triglycerides
% Change in HDL cholesterol at 12 weeks
7.9 % Change
Standard Deviation 15.3
2.7 % Change
Standard Deviation 9.9
Increased HDL-Cholesterol and Decreased Triglycerides
% Change in HDL cholesterol at 24 weeks
15.7 % Change
Standard Deviation 5.9
-1.5 % Change
Standard Deviation 3.8
Increased HDL-Cholesterol and Decreased Triglycerides
% Change in triglycerides at 12 weeks
-10.9 % Change
Standard Deviation 26.6
7.4 % Change
Standard Deviation 33.5
Increased HDL-Cholesterol and Decreased Triglycerides
% Change in triglycerides at 24 weeks
-15.4 % Change
Standard Deviation 6.3
19.7 % Change
Standard Deviation 11.3

SECONDARY outcome

Timeframe: 24 weeks

Lipoproteins will be isolated and analyzed using the gradient ultracentrifugation-high pressure liquid chromatography technique to isolate very low-density lipoprotein (VLDL), intermediate density lipoprotein (IDL), LDL, and high density lipoprotein (HDL) subfractions. Protein and lipid compositions of HDL is determined

Outcome measures

Outcome measures
Measure
Pioglitazone Group
n=13 Participants
This is a baseline versus treatment study comparing subjects on pioglitazone to a matched group of subjects treated with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level The pioglitazone treatment regimen for this arm of the study: 30 mg daily for three weeks increase to 45 mg daily for 21 more weeks
Comparator Group
n=13 Participants
This group of subjects will be maintained on standard treatment with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level as group treated with pioglitazone.
HDL Apolipoprotein Levels at Study End-point
HDL-apoAI at end point
65.0 mg/dL
Standard Deviation 0.9
65.7 mg/dL
Standard Deviation 7.0
HDL Apolipoprotein Levels at Study End-point
HDL-apoAII at end point
26.6 mg/dL
Standard Deviation 4.8
22.6 mg/dL
Standard Deviation 4.3
HDL Apolipoprotein Levels at Study End-point
HDL-apoCI at end point
10.9 mg/dL
Standard Deviation 2.2
8.4 mg/dL
Standard Deviation 2.5
HDL Apolipoprotein Levels at Study End-point
HDL-apoCII at end point
3.5 mg/dL
Standard Deviation 1.3
2.8 mg/dL
Standard Deviation 1.3
HDL Apolipoprotein Levels at Study End-point
HDL-apoCIII at end point
11.8 mg/dL
Standard Deviation 4.0
12.5 mg/dL
Standard Deviation 2.6
HDL Apolipoprotein Levels at Study End-point
HDL-apoM at end point
0.62 mg/dL
Standard Deviation 0.2
0.43 mg/dL
Standard Deviation 0.2

SECONDARY outcome

Timeframe: 24 weeks

The ability of serum HDL to remove cholesterol from cultured cells will be assessed as an in vitro method to evaluate a functional changes in HDL mediated by changes due to pioglitazone treatment. Cells were incubated with 2% serum from each study subject diluted in culture medium and incubations were performed for a total of 4 hours. Cholesterol efflux was calculated as the percent of cholesterol removed from the cells and appearing in the culture medium normalized to a reference serum pool as described in detail by de la Llera-Moya et al (de la Llera-Moya M, Drazul-Schrader D, Asztalos BF, Cuchel M, Rader DJ, Rothblat GH. The ability to promote efflux via ABCA1 determines the capacity of serum specimens with similar high-density lipoprotein cholesterol to remove cholesterol from macrophages. Arterioscler Thromb Vasc Biol. 2010 Apr;30(4):796-801. doi: 10.1161/ATVBAHA.109.199158. PMID: 20075420).

Outcome measures

Outcome measures
Measure
Pioglitazone Group
n=13 Participants
This is a baseline versus treatment study comparing subjects on pioglitazone to a matched group of subjects treated with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level The pioglitazone treatment regimen for this arm of the study: 30 mg daily for three weeks increase to 45 mg daily for 21 more weeks
Comparator Group
n=13 Participants
This group of subjects will be maintained on standard treatment with either metformin or sulfonylurea with the intent of controlling blood sugar to a comparable level as group treated with pioglitazone.
Cholesterol Efflux Capacity of HDL
1.02 Ratio
Standard Deviation 0.10
1.05 Ratio
Standard Deviation 0.07

Adverse Events

Pioglitazone Group

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Comparator Group

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Armando J. Mendez, Ph.D.

University of Miami Miller School of Medicine

Phone: 305-243-5342

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place

Restriction type: LTE60