Safety and Efficacy in LPL-Deficient Subjects of AMT-011, an Adeno-Associated Viral Vector Expressing Human Lipoprotein Lipase [S447X]

NCT ID: NCT01109498

Last Updated: 2011-09-30

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: PHASE2/PHASE3
• Total Enrollment: 14 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2007-08-31
• Study Completion Date: 2013-06-30

Brief Summary

LPLD is a rare autosomal recessive disorder, characterized by the presence of marked chylomicronemia and hence hypertriglyceridemia. Clinically the most severe manifestation of chylomicronemia, is acute pancreatitis, which can be lethal. There is no effective therapy available to modulate the course of the illness and prevent complications for these patients. The current clinical management consists of severe reduction of dietary fat that is hard if not almost impossible to comply with. LPLD subjects continue to experience pancreatitis attacks, and are admitted to intensive care units on several occasions.

Alipogene tiparvovec corrects or restores lipoprotein lipase (LPL) function long term, and hence reverses some symptoms, halts the disease progression and prevents further complications. Alipogene tiparvovec gene therapy ensures that a catabolically beneficial variant of the human LPL gene, LPL[S447X] is expressed and active in the relevant tissues in humans. Delivery of the gene is realized via intramuscular injection of an adeno-associated viral vector, pseudotyped with AAV1 capsids.

Detailed Description

The CT-AMT-011-01 study is an open-label, dose-escalating study evaluating the safety and efficacy of a single intramuscular administration of AMT-011 (at multiple sites). The study will be performed in the Community Genomic medicineCenter (CGMC) Chicoutimi, Canada, under the supervision of their medical ethical committee and according to the local biosafety procedures. The study participants will be treated under the responsibility of a Principal Investigator specialised in the treatment of lipid disorders. A total number of 14 subjects will be administered. Participants will be screened 3 weeks prior to administration of AMT-011 and will be evaluated for 12 weeks post administration in this study. After the study, subjects will be followed up long term with particular emphasis on the safety and efficacy aspects of LPL gene therapy using AMT-011. Subjects will be evaluated at the clinical site at 19 weeks, 26 weeks, 39 weeks, 1 year, 1.5 years, 2 years, 3 years, 4 years and 5 years after administration of AMT-011. The TG values that are obtained at week 26 will be used for secondary efficacy analysis.

Conditions

Familial Lipoprotein Lipase Deficiency

Keywords

LPLD; Lipoprotein Lipase Deficiency; Chylomicronaemia; Genetherapy; AAV; Alipogene Tiparvovec; AMT-011; Lipoprotein Lipase

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Dose cohort 3 x 10^11gc/kg

intra muscular, 3 x E11 gc per kg body weight, injected in a single series of intramuscular injections

Group Type: EXPERIMENTAL

Alipogene Tiparvovec (AMT-011), Human LPL [S447X]

Intervention Type: GENETIC

intra muscular, 3 x E11 gc per kg body weight, injected in a single series of intramuscular injections

Alipogene Tiparvovec, Human LPL [S447X]

intra muscular, 3 x E11 gc per kg body weight, injected in a single series of intramuscular injections with immunosuppressants

Group Type: EXPERIMENTAL

Mycophenolate mofetil

Intervention Type: DRUG

oral, 2 g/day, day -3 till week 12

Alipogene Tiparvovec (AMT-011), Human LPL [S447X]

Intervention Type: GENETIC

intra muscular, 3 x E11 gc per kg body weight, injected in a single series of intramuscular injections

cyclosporine

Intervention Type: DRUG

oral, 3 mg/kg/day, day -3 till week 12

Alipogene Tiparvovec, Human LPL [S447X], 1xE12 gc/kg

intra muscular, 1 x E12 gc per kg body weight, injected in a single series of intramuscular injections with immunosuppressants

Group Type: EXPERIMENTAL

Alipogene Tiparvovec (AMT-011), Human LPL [S447X]

Intervention Type: GENETIC

intra muscular, 1 x E12 gc per kg body weight, injected in a single series of intramuscular injections

Interventions

Alipogene Tiparvovec (AMT-011), Human LPL [S447X]

intra muscular, 1 x E12 gc per kg body weight, injected in a single series of intramuscular injections

Intervention Type: GENETIC

Mycophenolate mofetil

oral, 2 g/day, day -3 till week 12

Intervention Type: DRUG

Alipogene Tiparvovec (AMT-011), Human LPL [S447X]

intra muscular, 3 x E11 gc per kg body weight, injected in a single series of intramuscular injections

Intervention Type: GENETIC

cyclosporine

oral, 3 mg/kg/day, day -3 till week 12

Intervention Type: DRUG

Other Intervention Names

Glybera, AMT-011; CellCept; Glybera, AMT 011; Neoral

Eligibility Criteria

Inclusion Criteria

• Eligible Population Study participants must have participated in the preceding observation study (Prep-02: Appendix III) and be diagnosed with lipoprotein lipase deficiency, meeting the following criteria: (I) Their lipoprotein lipase activity levels in post-heparin plasma should be ≤20 % of normal; (II) Confirmed homozygocity or compound heterozygocity for mutations in the LPL gene; (III) Post-heparin plasma LPL mass should be >5% of normal; (IV) Median fasting plasma TG concentrations >10.00mmol/L, as determined on the basis of 5 consecutive time points in the preceding observation study with a history of pancreatitis.
• General Health The participant must be in good general physical health with, in the opinion of the investigator, no other clinically significant and relevant abnormalities of medical history, and no abnormalities at the physical examination and routine laboratory evaluation performed prior to the trial.
• Age Age ≥18 years old.
• Sex Male or female. Females must be of non-child bearing potential or with a negative pregnancy test and not breast feeding. Female subjects must use appropriate contraception (if relevant) and their spouse must use barrier contraception for the duration of the study (12 weeks). Males must practice barrier birth control and their spouse should use appropriate contraception until three consecutive semen samples, taken at least 75 days after administration, are negative for AMT-011 vector DNA.
• Compliance The participant is willing to fully comply with all study procedures and requirements of the trial such as restrictions to a low-fat diet (see section 8.1).
• Consent The participant has the mental ability to give voluntary written informed consent to participate in the study.

Exclusion Criteria

• Disease
• Apolipoprotein CII deficiency.
• Inflammatory muscle disease (e.g. myositis, myopathies or rhabdomolysis).
• Any current or relevant previous history of serious, severe or unstable physical or psychiatric illness, any medical disorder that may make the participant unlikely to fully complete the study, or any condition that presents undue risk from the study medication or procedures (e.g. malignant neoplasia).
• Active infectious disease of any nature, including clinically active viral infections.
• Laboratory Parameters

The following blood screening tests will result in exclusion from participation:

• Platelet count < 100 x 109 /L.
• Hemoglobin < 7.0 mmol/L.
• Liver function disturbances (bilirubin >2.50 x normal, transaminases >3 x ULN).
• CPK > 3 x ULN.
• Creatinine > 3 x ULN.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

International Antiviral Therapy Evaluation Center

OTHER

Sponsor Role: collaborator

Amsterdam Molecular Therapeutics

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Ecogene-21 Clinical Trial Center/ Centre de santé et de services sociaux de Chicoutimi

Chicoutimi, Quebec, Canada

Countries

Canada

References

Rip J, Nierman MC, Sierts JA, Petersen W, Van den Oever K, Van Raalte D, Ross CJ, Hayden MR, Bakker AC, Dijkhuizen P, Hermens WT, Twisk J, Stroes E, Kastelein JJ, Kuivenhoven JA, Meulenberg JM. Gene therapy for lipoprotein lipase deficiency: working toward clinical application. Hum Gene Ther. 2005 Nov;16(11):1276-86. doi: 10.1089/hum.2005.16.1276.

Reference Type: BACKGROUND

PMID: 16259561 (View on PubMed)

Ross CJ, Twisk J, Meulenberg JM, Liu G, van den Oever K, Moraal E, Hermens WT, Rip J, Kastelein JJ, Kuivenhoven JA, Hayden MR. Long-term correction of murine lipoprotein lipase deficiency with AAV1-mediated gene transfer of the naturally occurring LPL(S447X) beneficial mutation. Hum Gene Ther. 2004 Sep;15(9):906-19. doi: 10.1089/hum.2004.15.906.

Reference Type: BACKGROUND

PMID: 15353045 (View on PubMed)

Ross CJ, Liu G, Kuivenhoven JA, Twisk J, Rip J, van Dop W, Excoffon KJ, Lewis SM, Kastelein JJ, Hayden MR. Complete rescue of lipoprotein lipase-deficient mice by somatic gene transfer of the naturally occurring LPLS447X beneficial mutation. Arterioscler Thromb Vasc Biol. 2005 Oct;25(10):2143-50. doi: 10.1161/01.ATV.0000176971.27302.b0. Epub 2005 Jul 7.

Reference Type: BACKGROUND

PMID: 16002740 (View on PubMed)

Ross CJ, Twisk J, Bakker AC, Miao F, Verbart D, Rip J, Godbey T, Dijkhuizen P, Hermens WT, Kastelein JJ, Kuivenhoven JA, Meulenberg JM, Hayden MR. Correction of feline lipoprotein lipase deficiency with adeno-associated virus serotype 1-mediated gene transfer of the lipoprotein lipase S447X beneficial mutation. Hum Gene Ther. 2006 May;17(5):487-99. doi: 10.1089/hum.2006.17.487.

Reference Type: BACKGROUND

PMID: 16716106 (View on PubMed)

Stroes ES, Nierman MC, Meulenberg JJ, Franssen R, Twisk J, Henny CP, Maas MM, Zwinderman AH, Ross C, Aronica E, High KA, Levi MM, Hayden MR, Kastelein JJ, Kuivenhoven JA. Intramuscular administration of AAV1-lipoprotein lipase S447X lowers triglycerides in lipoprotein lipase-deficient patients. Arterioscler Thromb Vasc Biol. 2008 Dec;28(12):2303-4. doi: 10.1161/ATVBAHA.108.175620. Epub 2008 Sep 18. No abstract available.

Reference Type: BACKGROUND

PMID: 18802015 (View on PubMed)

Burnett JR, Hooper AJ. Alipogene tiparvovec, an adeno-associated virus encoding the Ser(447)X variant of the human lipoprotein lipase gene for the treatment of patients with lipoprotein lipase deficiency. Curr Opin Mol Ther. 2009 Dec;11(6):681-91.

Reference Type: BACKGROUND

PMID: 20072945 (View on PubMed)

Gaudet D, Methot J, Dery S, Brisson D, Essiembre C, Tremblay G, Tremblay K, de Wal J, Twisk J, van den Bulk N, Sier-Ferreira V, van Deventer S. Efficacy and long-term safety of alipogene tiparvovec (AAV1-LPLS447X) gene therapy for lipoprotein lipase deficiency: an open-label trial. Gene Ther. 2013 Apr;20(4):361-9. doi: 10.1038/gt.2012.43. Epub 2012 Jun 21.

Reference Type: DERIVED

PMID: 22717743 (View on PubMed)

Other Identifiers

CT-AMT-011-01

Identifier Type: -

Identifier Source: org_study_id