Trial Outcomes & Findings for Defining the Intestinal Microbiota in Premature Neonates (NCT NCT01102738)

NCT ID: NCT01102738

Last Updated: 2020-05-01

Results Overview

Faecal samples were analysed using 16S rRNA gene sequencing to determine the bacterial content present in faecal samples collected from pre term infants prior to the onset of necrotising enterocolitis. Bacteria were identified and relative proportions reported for each faecal sample analysed.

Recruitment status

COMPLETED

Target enrollment

369 participants

Primary outcome timeframe

Maximum of 6 months - serial samples collected from each infant (maximum admission duration 6 months), recruitment opened for 24 months.

Results posted on

2020-05-01

Participant Flow

Participant milestones

Participant milestones
Measure
Premature Babies (<32 Weeks)
All premature babies born at less than 32 completed weeks gestation who are admitted to an Imperial College NHS Healthcare Trust Neonatal Intensive Care Unit (St. Mary's Hospital or Queen Charlotte's \& Chelsea Hospital), and whose parents/guardians have given their consent will be eligible to enter the study.
Overall Study
STARTED
369
Overall Study
COMPLETED
369
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Defining the Intestinal Microbiota in Premature Neonates

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Premature Babies (<32 Weeks)
n=369 Participants
All premature babies born at less than 32 completed weeks gestation who are admitted to an Imperial College NHS Healthcare Trust Neonatal Intensive Care Unit (St. Mary's Hospital or Queen Charlotte's \& Chelsea Hospital), and whose parents/guardians have given their consent will be eligible to enter the study.
Age, Categorical
<=18 years
369 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
0 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
Age, Continuous
2 days
n=5 Participants
Sex: Female, Male
Female
171 Participants
n=5 Participants
Sex: Female, Male
Male
198 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
2 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
339 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
28 Participants
n=5 Participants
Region of Enrollment
United Kingdom
369 participants
n=5 Participants

PRIMARY outcome

Timeframe: Maximum of 6 months - serial samples collected from each infant (maximum admission duration 6 months), recruitment opened for 24 months.

Population: Faecal samples and clinical data were collected from all participants (369 infants) recruited into the study. Samples were only analysed from infants who developed NEC (n=12) and 3 matched controls (n=36).

Faecal samples were analysed using 16S rRNA gene sequencing to determine the bacterial content present in faecal samples collected from pre term infants prior to the onset of necrotising enterocolitis. Bacteria were identified and relative proportions reported for each faecal sample analysed.

Outcome measures

Outcome measures
Measure
Premature Babies (<32 Weeks)
n=48 Participants
All premature babies born at less than 32 completed weeks gestation who are admitted to an Imperial College NHS Healthcare Trust Neonatal Intensive Care Unit (St. Mary's Hospital or Queen Charlotte's \& Chelsea Hospital), and whose parents/guardians have given their consent will be eligible to enter the study.
The Composition of Bacteria Present, Established by Ultra-deep RNA Gene Sequencing, in Pre-morbid Faecal Samples From Neonates With Necrotizing Enterocolitis and Late-onset Bacterial Sepsis.
Klebsiella OTU associated NEC
7 Participants
The Composition of Bacteria Present, Established by Ultra-deep RNA Gene Sequencing, in Pre-morbid Faecal Samples From Neonates With Necrotizing Enterocolitis and Late-onset Bacterial Sepsis.
Clostridium associated NEC
4 Participants
The Composition of Bacteria Present, Established by Ultra-deep RNA Gene Sequencing, in Pre-morbid Faecal Samples From Neonates With Necrotizing Enterocolitis and Late-onset Bacterial Sepsis.
Non Klebsiella or Clostridium associated NEC
1 Participants
The Composition of Bacteria Present, Established by Ultra-deep RNA Gene Sequencing, in Pre-morbid Faecal Samples From Neonates With Necrotizing Enterocolitis and Late-onset Bacterial Sepsis.
Controls
36 Participants

Adverse Events

Premature Babies (<32 Weeks)

Serious events: 0 serious events
Other events: 0 other events
Deaths: 30 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Kathleen Sim

Imperial College London

Phone: (+44) (0)20 7594 3695

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place