Trial Outcomes & Findings for Use of PRO Onc Assay to Assess HER2 in Patients With Metastatic Breast Cancer (NCT NCT01048099)

NCT ID: NCT01048099

Last Updated: 2016-01-29

Results Overview

The percentage of HER2-negative metastatic breast cancer (MBC) patients having an objective benefit from treatment per Response Evaluation Criteria In Solid Tumors Criteria (RECIST v1.0) for target lesions and assessed by MRI or CT: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), \>=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR. Includes patients with HER2 overexpression/activation as detected by PRO Onc Assay.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

283 participants

Primary outcome timeframe

18 months

Results posted on

2016-01-29

Participant Flow

Pts with HER2-negative, metastatic breast cancer were enrolled. Blood was collected to perform the PRO Onc Circulating Tumor Cell (CTC). Pts without CTCs present were taken off-study. Following the assay, pts without detected HER2 overexpression/activation were taken off-study. Of the remaining pts, those FISH-positive for HER2 came off-study.

Participant milestones

Participant milestones
Measure
All Patients
Patients Enrolled and Blood Collected
STARTED
283
Patients Enrolled and Blood Collected
COMPLETED
226
Patients Enrolled and Blood Collected
NOT COMPLETED
57
Patients Evaluated by PRO Onc Assay
STARTED
226
Patients Evaluated by PRO Onc Assay
COMPLETED
24
Patients Evaluated by PRO Onc Assay
NOT COMPLETED
202
Pts w/ HER2 Overexpression/Activation
STARTED
24
Pts w/ HER2 Overexpression/Activation
COMPLETED
14
Pts w/ HER2 Overexpression/Activation
NOT COMPLETED
10
Treatment Per Protocol
STARTED
14
Treatment Per Protocol
COMPLETED
0
Treatment Per Protocol
NOT COMPLETED
14

Reasons for withdrawal

Reasons for withdrawal
Measure
All Patients
Patients Enrolled and Blood Collected
Patients without circulating tumor cells
57
Patients Evaluated by PRO Onc Assay
Patients with no HER2 abnormality
202

Baseline Characteristics

Use of PRO Onc Assay to Assess HER2 in Patients With Metastatic Breast Cancer

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Patients Treated
n=14 Participants
Patients who received study treatment
Age, Continuous
65 years
n=5 Participants
Sex: Female, Male
Female
14 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
Region of Enrollment
United States
14 participants
n=5 Participants

PRIMARY outcome

Timeframe: 18 months

The percentage of HER2-negative metastatic breast cancer (MBC) patients having an objective benefit from treatment per Response Evaluation Criteria In Solid Tumors Criteria (RECIST v1.0) for target lesions and assessed by MRI or CT: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), \>=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR. Includes patients with HER2 overexpression/activation as detected by PRO Onc Assay.

Outcome measures

Outcome measures
Measure
All Patients Evaluated by PRO Onc Assay
n=14 Participants
Part II: Objective Response Rate of HER2-negative Metastatic Breast Cancer (by FISH Testing)
7 percentage of participants

PRIMARY outcome

Timeframe: 18 months

Population: Includes patients with HER2 overexpression detected by the PRO Onc Assay

The percentage of patients having an objective benefit from treatment per Response Evaluation Criteria In Solid Tumors Criteria (RECIST v1.0) for target lesions and assessed by MRI or CT: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), \>=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR. Includes patients with HER2 overexpression as identified by the PRO Onc Assay.

Outcome measures

Outcome measures
Measure
All Patients Evaluated by PRO Onc Assay
n=10 Participants
Part II: Objective Response Rate of Trastuzumab Therapy
10 percentage of participants

PRIMARY outcome

Timeframe: 18 months

Population: Includes patients with only HER2 activation (no overexpression) as detected by the PRO Onc Assay

The percentage of patients with HER2 activation (no overexpression) as identified by the PRO Onc Assay who experience an objective benefit from treatment, per Response Evaluation Criteria In Solid Tumors Criteria (RECIST v1.0) for target lesions and assessed by MRI or CT: Complete Response (CR), Disappearance of all target lesions; Partial Response (PR), \>=30% decrease in the sum of the longest diameter of target lesions; Overall Response (OR) = CR + PR.

Outcome measures

Outcome measures
Measure
All Patients Evaluated by PRO Onc Assay
n=4 Participants
Part II: Objective Response Rate of Pertuzumab Therapy
0 percentage of participants

SECONDARY outcome

Timeframe: 12 months

Includes patients with HER2-negative metastatic breast cancer (MBC) as determined by FISH testing.

Outcome measures

Outcome measures
Measure
All Patients Evaluated by PRO Onc Assay
n=226 Participants
Part 1: The Incidence of HER2 Overexpression/Activation as Measured by the PRO Onc Assay
HER2 overexpressed
11 participants
Part 1: The Incidence of HER2 Overexpression/Activation as Measured by the PRO Onc Assay
HER2 activated
10 participants
Part 1: The Incidence of HER2 Overexpression/Activation as Measured by the PRO Onc Assay
HER2 overexpressed and activated
3 participants
Part 1: The Incidence of HER2 Overexpression/Activation as Measured by the PRO Onc Assay
No HER2 overexpression/activation detected
202 participants

SECONDARY outcome

Timeframe: 12 months

Population: Includes all patients with blood drawn and analyzed for CTCs

Percentage of HER2-negative MBC patients (identified by FISH testing) having CTCs present in blood specimens.

Outcome measures

Outcome measures
Measure
All Patients Evaluated by PRO Onc Assay
n=283 Participants
Part I: The Incidence of Isolation of Circulating Tumor Cells (CTCs) From Blood Specimens
80 percentage of participants

Adverse Events

All Treated Patients

Serious events: 5 serious events
Other events: 13 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
All Treated Patients
n=14 participants at risk
Includes all patients with HER2 overexpression/activation (as identified by the PRO Onc Assay) who received study treatment
General disorders
General disorders and administration site conditions - Other, disease progression
14.3%
2/14 • Number of events 2 • 18 Months
Gastrointestinal disorders
Ileus
7.1%
1/14 • Number of events 1 • 18 Months
Renal and urinary disorders
Acute kidney injury
7.1%
1/14 • Number of events 1 • 18 Months
Infections and infestations
Infections and infestations - Other, pneumonia
7.1%
1/14 • Number of events 2 • 18 Months

Other adverse events

Other adverse events
Measure
All Treated Patients
n=14 participants at risk
Includes all patients with HER2 overexpression/activation (as identified by the PRO Onc Assay) who received study treatment
General disorders
Fatigue
50.0%
7/14 • 18 Months
General disorders
Infusion related reaction
28.6%
4/14 • 18 Months
Infections and infestations
Urinary Tract Infection
28.6%
4/14 • 18 Months
Metabolism and nutrition disorders
Anorexia
21.4%
3/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Dyspnea
21.4%
3/14 • 18 Months
Gastrointestinal disorders
Nausea
21.4%
3/14 • 18 Months
Gastrointestinal disorders
Abdominal pain
14.3%
2/14 • 18 Months
Immune system disorders
Allergic reaction
14.3%
2/14 • 18 Months
Blood and lymphatic system disorders
Anemia
14.3%
2/14 • 18 Months
Gastrointestinal disorders
Constipation
14.3%
2/14 • 18 Months
Metabolism and nutrition disorders
Dehydration
14.3%
2/14 • 18 Months
Gastrointestinal disorders
Diarrhea
14.3%
2/14 • 18 Months
General disorders
Edema
14.3%
2/14 • 18 Months
Skin and subcutaneous tissue disorders
Rash
14.3%
2/14 • 18 Months
Renal and urinary disorders
Acute kidney injury
7.1%
1/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Allergic rhinitis
7.1%
1/14 • 18 Months
Skin and subcutaneous tissue disorders
Alopecia
7.1%
1/14 • 18 Months
Psychiatric disorders
Anxiety
7.1%
1/14 • 18 Months
Gastrointestinal disorders
Ascites
7.1%
1/14 • 18 Months
General disorders
Non-cardiac chest pain
7.1%
1/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Cough
7.1%
1/14 • 18 Months
Vascular disorders
Thromboembolic event
7.1%
1/14 • 18 Months
General disorders
Fever
7.1%
1/14 • 18 Months
Nervous system disorders
Headache
7.1%
1/14 • 18 Months
Renal and urinary disorders
Renal and urinary disorders - Other, hydronephrosis
7.1%
1/14 • 18 Months
Endocrine disorders
Hyperthyroidism
7.1%
1/14 • 18 Months
Endocrine disorders
Hypothyroidism
7.1%
1/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Hypoxia
7.1%
1/14 • 18 Months
Gastrointestinal disorders
Ileus
7.1%
1/14 • 18 Months
Psychiatric disorders
Insomnia
7.1%
1/14 • 18 Months
Hepatobiliary disorders
Hepatobiliary disorders - Other, liver dysfunction
7.1%
1/14 • 18 Months
Musculoskeletal and connective tissue disorders
Generalized muscle weakness
7.1%
1/14 • 18 Months
Nervous system disorders
Peripheral sensory neuropathy
7.1%
1/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Pulmonary hypertension
7.1%
1/14 • 18 Months
Infections and infestations
Skin infection
7.1%
1/14 • 18 Months
Nervous system disorders
Dysgeusia
7.1%
1/14 • 18 Months
Respiratory, thoracic and mediastinal disorders
Respiratory, thoracic and mediastinal disorders - Other, thoracic pain
7.1%
1/14 • 18 Months
Investigations
Platelet count decreased
7.1%
1/14 • 18 Months
Renal and urinary disorders
Urinary frequency
7.1%
1/14 • 18 Months
Investigations
Weight loss
7.1%
1/14 • 18 Months

Additional Information

John D. Hainsworth, MD

Sarah Cannon Research Institute

Phone: 1-877-691-7274

Results disclosure agreements

  • Principal investigator is a sponsor employee The sponsor can review/embargo results communications prior to public release for a period that is \>60 but =180 days from date submitted to sponsor, who may require changes to the communication in order to remove specifically identified confidential information (other than study data) and/or delay the proposed publication to enable the sponsor to seek patent protection for inventions. The PI may not publish its results until 18 mos. after the trial has been completed at all sites
  • Publication restrictions are in place

Restriction type: OTHER