An Evaluation Of Three Dose Levels Of 3-Antigen Staphylococcus Aureus Vaccine (SA3Ag) In Healthy Adults

NCT ID: NCT01018641

Last Updated: 2014-04-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

449 participants

Study Classification

INTERVENTIONAL

Study Start Date

2010-01-31

Study Completion Date

2011-07-31

Brief Summary

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This study is a first-in-human (Phase 1) study using three dose levels of an investigational vaccine directed against Staphylococcus aureus (SA3Ag). This study is primarily designed to assess how safe and well tolerated SA3Ag is, but will also describe the immune response over 12 months elicited by SA3Ag. Additionally, this study will assess the effect of SA3Ag vaccine on the number of Staphylococcus aureus bacteria that naturally occur on the skin and within the nose and throat.

Detailed Description

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Conditions

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Bacterial Infections Staphylococcal Vaccines Immunotherapy, Active Staphylococcal Skin Infections Staphylococcal Infections

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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1

SA3Ag in both stage 1 and stage 2

Group Type EXPERIMENTAL

SA3Ag vaccine

Intervention Type BIOLOGICAL

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive 0.5 mL IM of the same dose level he/she received in stage 1.

Blood draw

Intervention Type PROCEDURE

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Colonization swab samples

Intervention Type PROCEDURE

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

2

SA3Ag in stage 1 followed by placebo in stage 2.

Group Type EXPERIMENTAL

SA3Ag followed by Placebo

Intervention Type BIOLOGICAL

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive one injection of 0.5 mL IM of the placebo.

Blood draw

Intervention Type PROCEDURE

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Colonization swab samples

Intervention Type PROCEDURE

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

3

Placebo in both stage 1 and stage 2

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type BIOLOGICAL

In both stage 1 and stage 2, recipients will receive one injection (0.5 mL) IM of 150 mM (isotonic) NaCl for a total of 2 injections throughout the study.

Blood draw

Intervention Type PROCEDURE

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Colonization swab samples

Intervention Type PROCEDURE

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

4

SA3Ag in stage 1 and no vaccine in stage 2.

Group Type EXPERIMENTAL

SA3Ag with no booster in stage 2

Intervention Type BIOLOGICAL

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive no vaccine.

Blood draw

Intervention Type PROCEDURE

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Colonization swab samples

Intervention Type PROCEDURE

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

5

Placebo in stage 1 and no vaccine in stage 2.

Group Type PLACEBO_COMPARATOR

Placebo with no booster in stage 2

Intervention Type PROCEDURE

In stage 1, recipients will receive one injection (0.5 mL) IM of 150 mM (isotonic) NaCl.

In stage 2 the subject will receive no vaccine.

Blood draw

Intervention Type PROCEDURE

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Colonization swab samples

Intervention Type PROCEDURE

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Interventions

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SA3Ag vaccine

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive 0.5 mL IM of the same dose level he/she received in stage 1.

Intervention Type BIOLOGICAL

Blood draw

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Intervention Type PROCEDURE

Colonization swab samples

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Intervention Type PROCEDURE

SA3Ag followed by Placebo

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive one injection of 0.5 mL IM of the placebo.

Intervention Type BIOLOGICAL

Blood draw

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Intervention Type PROCEDURE

Colonization swab samples

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Intervention Type PROCEDURE

Placebo

In both stage 1 and stage 2, recipients will receive one injection (0.5 mL) IM of 150 mM (isotonic) NaCl for a total of 2 injections throughout the study.

Intervention Type BIOLOGICAL

Blood draw

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Intervention Type PROCEDURE

Colonization swab samples

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Intervention Type PROCEDURE

SA3Ag with no booster in stage 2

In stage 1, each subject will receive 1 injection (0.5 mL) of one of the following doses:

Low dose level 10 μg of CP5 and CP8 and 20 μg of rClfAm Mid-dose level 30 μg of CP5 and CP8 and 60 μg of rClfAm High dose level 100 μg of CP5 and CP8 and 200 μg of rClfAm

In stage 2 the subject will receive no vaccine.

Intervention Type BIOLOGICAL

Blood draw

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Intervention Type PROCEDURE

Colonization swab samples

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Intervention Type PROCEDURE

Placebo with no booster in stage 2

In stage 1, recipients will receive one injection (0.5 mL) IM of 150 mM (isotonic) NaCl.

In stage 2 the subject will receive no vaccine.

Intervention Type PROCEDURE

Blood draw

Blood for immunogenicity will be taken at timepoints throughout stage 1 and stage 2. Blood for hematology will be done in a select subset of subjects in stage 1.

Intervention Type PROCEDURE

Colonization swab samples

Colonization swabs will be taken at timepoints throughout stage 1 and stage 2.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Healthy adults aged 18 to 24 years or 50 to 85 years who are available for the entire duration of the study, able to be contacted by phone, and able to complete all study procedures, including completion of an electronic diary (e-diary).
* Men and women who are able to have children, must use a reliable method of birth control for the duration of the study.

Exclusion Criteria

* Any major illness that would substantially increase the risk associated with participation in the study, or interfere with the evaluation of the study objectives - this is determined by the local physician.
* Donation of 250 mL or more of blood within the last 3 months.
* Condition associated with prolonged bleeding time, including subjects taking anticoagulant medication or antiplatelet therapy.
* Any contraindication to vaccination or vaccine components.
* Immunocompromised persons and subjects who receive treatment with immunosuppressive therapy.
* Previous administration of S. aureus vaccination.
* Receipt of blood products or immunoglobulins within 12 months prior to study
* Participation in another trial (not including observational trials) within the last 30 days.
* Study site personnel or immediate family members (first-degree relatives).
* Women who are pregnant (as determined by urine pregnancy test) or breast-feeding.
* Residence in a nursing home or long-term care facility or requirement for semiskilled nursing care.
* For subjects aged 65 years or older, a Mini-Mental State Examination (MMSE) score of \<=21.
Minimum Eligible Age

18 Years

Maximum Eligible Age

85 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Pfizer

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Pfizer CT.gov Call Center

Role: STUDY_DIRECTOR

Pfizer

Locations

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Pfizer Investigational Site

Herston, Queensland, Australia

Site Status

Pfizer Investigational Site

Adelaide, South Australia, Australia

Site Status

Pfizer Investigational Site

North Adelaide, South Australia, Australia

Site Status

Pfizer Investigational Site

Prahran, Victoria, Australia

Site Status

Pfizer Investigational Site

Subiaco, Western Australia, Australia

Site Status

Countries

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Australia

References

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Marshall H, Nissen M, Richmond P, Shakib S, Jiang Q, Cooper D, Rill D, Baber J, Eiden J, Gruber WC, Jansen KU, Anderson AS, Zito ET, Girgenti D. Safety and immunogenicity of a booster dose of a 3-antigen Staphylococcus aureus vaccine (SA3Ag) in healthy adults: A randomized phase 1 study. J Infect. 2016 Nov;73(5):437-454. doi: 10.1016/j.jinf.2016.08.004. Epub 2016 Aug 9.

Reference Type DERIVED
PMID: 27519620 (View on PubMed)

Nissen M, Marshall H, Richmond P, Shakib S, Jiang Q, Cooper D, Rill D, Baber J, Eiden J, Gruber W, Jansen KU, Emini EA, Anderson AS, Zito ET, Girgenti D. A randomized phase I study of the safety and immunogenicity of three ascending dose levels of a 3-antigen Staphylococcus aureus vaccine (SA3Ag) in healthy adults. Vaccine. 2015 Apr 8;33(15):1846-54. doi: 10.1016/j.vaccine.2015.02.024. Epub 2015 Feb 21.

Reference Type DERIVED
PMID: 25707693 (View on PubMed)

Related Links

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Other Identifiers

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B2251002

Identifier Type: -

Identifier Source: secondary_id

6123K1-1007

Identifier Type: -

Identifier Source: org_study_id

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