Trial Outcomes & Findings for Immunization With HIV-1 Peptides in Adjuvant for Treatment of Patients With Chronic HIV-infection (NCT NCT01009762)

NCT ID: NCT01009762

Last Updated: 2014-03-27

Results Overview

the numbers of treatment related side effects (DLT = reaction 3 or more) are registered for participants

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

11 participants

Primary outcome timeframe

up to 6 months after end of treatment

Results posted on

2014-03-27

Participant Flow

Participants were recruited at the infectious disease department at the University hospitals

20 participant numbers were randomised to vaccine or placebo but at the deadline for expiring of the vaccine only 11 participants were enrolled but all 11 completed the study

Participant milestones

Participant milestones
Measure
Saline
Sterile saline for injection is used as placebo arm. It is administered i.m. in the same way as for the active vaccine, week 0, 2, 4, 8.
AFO-18 Vaccinated
Patients receiving the experimental therapeutic vaccine
Overall Study
STARTED
1
10
Overall Study
Primary Outcome
0
0
Overall Study
COMPLETED
1
10
Overall Study
NOT COMPLETED
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Immunization With HIV-1 Peptides in Adjuvant for Treatment of Patients With Chronic HIV-infection

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Saline
n=1 Participants
Sterile saline for injection is used as placebo arm. It is administered i.m. in the same way as for the active vaccine, week 0, 2, 4, 8.
AFO-18 Vaccinated
n=10 Participants
Patients receiving the experimental therapeutic vaccine
Total
n=11 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
1 Participants
n=5 Participants
10 Participants
n=7 Participants
11 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Sex: Female, Male
Female
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Sex: Female, Male
Male
1 Participants
n=5 Participants
10 Participants
n=7 Participants
11 Participants
n=5 Participants
Region of Enrollment
Denmark
1 participants
n=5 Participants
10 participants
n=7 Participants
11 participants
n=5 Participants
Clusters of Differentiation 4 (CD4) T-cells >400 cells/mm^3
1 participants
n=5 Participants
10 participants
n=7 Participants
11 participants
n=5 Participants
Absence of T cell reaction to all vaccine peptides
1 participants
n=5 Participants
10 participants
n=7 Participants
11 participants
n=5 Participants
Viral load >10^3/mm^3 plasma
1 participants
n=5 Participants
10 participants
n=7 Participants
11 participants
n=5 Participants

PRIMARY outcome

Timeframe: up to 6 months after end of treatment

Population: Interview, questionaire, objective examination by medical doctor, blood testing for hematology, clinical chemistry, CD4 counts, HIV-1 viral load

the numbers of treatment related side effects (DLT = reaction 3 or more) are registered for participants

Outcome measures

Outcome measures
Measure
Vaccinee
n=10 Participants
participants receiving the vaccine
Placebo
n=1 Participants
participants receiving placebo (=saline)
Numbers of Treatment Related Side Effects (DLT = Reaction 3 or More)
0 side effects
0 side effects

SECONDARY outcome

Timeframe: 10-14 days or 3 months or 6 months after last immunisation

Population: Peripheral Blood Mononuclear Cells (PBMC) from blood was measured in IFNg-ELISPOT and/or Intracellular Cytokines (ICS) Flowcytometry for T cell responses. All 10 vaccinee developed a new T cell immune response to at least one vaccine epitope. The saline placebo did not develop any new t cell responses.

Number of Participants with New T Cell Response to the Vaccine Target Epitopes as Measured by Intracellular Cytokine Stain Flowcytometry (IC-FACS) and/or IFNg-ELISPOT Analysis. Criteria's for meeting anticipated secondary end-point was that \>50% of vaccinees reacted with new Clusters of differentiation 8 (CD8) T-cell and/or Clusters of differentiation 4 (CD4) T-cell response to al least one of the vaccine target epitopes as measured by IC-Facs and/or interferon-gamma (IFNg) - Enzyme-Linked ImmunoSpot (ELISPOT) assays.

Outcome measures

Outcome measures
Measure
Vaccinee
n=1 Participants
participants receiving the vaccine
Placebo
n=10 Participants
participants receiving placebo (=saline)
Number of Participants With New T Cell Response to the Vaccine Target Epitopes
0 participants
10 participants

SECONDARY outcome

Timeframe: up to 6 months after treatment stop

Population: the numbers of participants that showed changes (lowering of) in Viral load (measured as HIV-1 RNA copies/mm\^3 plasma in commercial quantitative PCR) at end of study (6 months after vaccination) relative to base-line viral-load was counted at 6 month after vaccination (end of study)

HIV-1 RNA Viral load was measured by Quantitative-PCR in plasma as numbers of virus RNA copies/mm\^3 relative to baseline viral-load for each participant. The numbers of participant with lowering of HIV RNA plasma Viral-load is counted at base-line and at 6 months (end of study) and provided in the table (analysis population description) and the number of participants that showed lowering of viral-load was counted. Criteria for this anticipated end-point was a significant lowering of HIV RNA viral-load in \>50% of responders (defined as participants with new T-cell responses).

Outcome measures

Outcome measures
Measure
Vaccinee
n=1 Participants
participants receiving the vaccine
Placebo
n=10 Participants
participants receiving placebo (=saline)
Numbers of Participants With Lowering of HIV RNA Viral-load
0 participants
0 participants

Adverse Events

Vaccinee

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Professor Anders Fomsgaard

Statens Serum Institut

Phone: +45-32683460

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place