Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response

NCT ID: NCT01005342

Last Updated: 2009-10-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

18 participants

Study Classification

INTERVENTIONAL

Study Start Date

2007-05-31

Study Completion Date

2007-06-30

Brief Summary

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The aim of this study is to

* Measure the effect on gene expression in leukocytes from a meal rich in oat bran
* Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers

Detailed Description

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Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits.

Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h.

NuGO Affymetrix Human Genechip NuGO\_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.

Conditions

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Hypoglycemia Hyperglycemia

Keywords

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gene expression oats rye sugar beet fiber glucose insulin triglyceride decreased postprandial glucose decreased postprandial insulin glycemic index

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

FACTORIAL

Blinding Strategy

SINGLE

Participants

Study Groups

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Mixture of fiber

Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber

Group Type EXPERIMENTAL

Mixture of fiber

Intervention Type OTHER

38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).

Sugar beet fiber

Single intake of sugar beet fiber

Group Type EXPERIMENTAL

Sugar beet fiber

Intervention Type OTHER

19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber).

Rye bran

Single intake of rye bran

Group Type EXPERIMENTAL

Rye bran

Intervention Type OTHER

31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber).

Oat bran

Single intake of oat bran

Group Type EXPERIMENTAL

Oat bran

Intervention Type OTHER

82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber).

Spray-dried oat drink

Single intake of spray-dried oat drink

Group Type EXPERIMENTAL

Spray-dried oat drink

Intervention Type OTHER

62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber).

Control

Single intake of a meal with no added fiber

Group Type PLACEBO_COMPARATOR

Control

Intervention Type OTHER

No fiber was added to control meal (250 g black-currant beverage)

Interventions

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Oat bran

82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber).

Intervention Type OTHER

Spray-dried oat drink

62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber).

Intervention Type OTHER

Rye bran

31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber).

Intervention Type OTHER

Sugar beet fiber

19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber).

Intervention Type OTHER

Mixture of fiber

38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).

Intervention Type OTHER

Control

No fiber was added to control meal (250 g black-currant beverage)

Intervention Type OTHER

Other Intervention Names

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Oats Beta-glucan Oats Oat milk Beta-glucan Rye Arabinoxylan Sugar beet Pectin

Eligibility Criteria

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Inclusion Criteria

* BMI 18-30

Exclusion Criteria

* pregnancy
* breastfeeding
* diabetes mellitus
* hepatitis B
* blood lipid lowering pharmaceuticals
* intolerance or allergy to cereals or sugar beet fiber
Minimum Eligible Age

20 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Vinnova

OTHER_GOV

Sponsor Role collaborator

Oatly AB

UNKNOWN

Sponsor Role collaborator

Lantmannen Food R&D

UNKNOWN

Sponsor Role collaborator

Danisco Sugar AB/Fibrex

UNKNOWN

Sponsor Role collaborator

Lund University

OTHER

Sponsor Role lead

Responsible Party

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Lund University

Principal Investigators

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Gunilla Önning, Dr.

Role: STUDY_DIRECTOR

Lund University

Locations

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Lund University

Lund, , Sweden

Site Status

Countries

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Sweden

References

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Ulmius M, Johansson A, Onning G. The influence of dietary fibre source and gender on the postprandial glucose and lipid response in healthy subjects. Eur J Nutr. 2009 Oct;48(7):395-402. doi: 10.1007/s00394-009-0026-x. Epub 2009 May 5.

Reference Type RESULT
PMID: 19415409 (View on PubMed)

Other Identifiers

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KCROS07

Identifier Type: -

Identifier Source: org_study_id