Study to Identify Mechanisms of Resistance to Standard Therapy in Patients With Metastatic Colorectal Cancer

NCT ID: NCT00984048

Last Updated: 2021-06-15

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 160 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2009-08-31
• Study Completion Date: 2019-08-31

Brief Summary

This is a multicenter translational study to understand therapeutic resistance in patients undergoing first-line chemotherapy (FOLFOX/Avastin, or FOLFIRI/Avastin) for metastatic colorectal cancer. Tissue samples from liver metastasis will be collected and banked before the start of chemotherapy and at the time of progression. Additionally, blood samples will be drawn monthly and stored in the tissue biobank.

Detailed Description

The major obstacle to the cure of cancer by pharmacological agents is resistance to these agents. Clinical responses of metastatic cancers to the most advanced chemotherapeutic agents usually range from 15 to 40%, indicating that intrinsic resistance, and acquired resistance occurs almost inevitably in those tumors that do respond. In patients with metastatic colorectal cancer, clinical resistance to a particular treatment is a clear endpoint (tumor growth), and is usually observed within 6-12 months of any given therapy. Thus, drug resistance and selecting appropriate therapeutic alternatives for drug-resistant cancer remain major dilemmas for oncologists.

The current first-line treatment for metastatic colorectal cancer in Quebec and much of North America is a combination called FOLFOX (the fluoro-pyrimidine 5-FU given as a 46-hour infusion, folinic acid and oxaliplatin) in combination with bevacizumab (Avastin®). An alternative regimen of cytotoxic drugs, also used with Avastin®, is FOLFIRI, which simply replaces oxaliplatin with the topoisomerase inhibitor irinotecan. In the metastatic setting, studies have not demonstrated significant differences between the two regimens, such that decision-making lacks definitive tools.

The objective of this study is to identify, in clinical samples, the molecular signature of clinically resistant colorectal cancer (CRC) patients for the most current and commonly used therapeutic agents. The goals of this study are two-fold. First, to build a biobank of blood and tissue specimens, prior to starting chemotherapy and at a determined time-point (progression of disease), from patients undergoing the same standard and well established first-line treatments (FOLFOX/bevacizumab or FOLFIRI/bevacizumab) for metastatic colorectal cancer. Second, to use state-of-the-art approaches by various collaborating laboratories to correlate clinical outcomes with molecular events that can be used to predict and circumscribe chemoresistance.

Conditions

Colorectal Cancer

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Study Groups

FOLFOX, XELOX or FOLFIRI +/- bevacizumab

Patients are scheduled to receive first-line treatment for metastatic disease. They should be receiving at least one component of either FOLFOX, XELOX or FOLFIRI regimen with or without bevacizumab.

Needle core biopsies of liver metastasis

Intervention Type: OTHER

No investigational products will be administered to subjects as part of this translational research study. A first-line chemotherapy regimen consisting of FOLFOX, XELOX or FOLFIRI +/- bevacizumab will be administered as per the standard of care at each treating institution. Needle core biopsies of liver metastasis will be collected and banked before the start of chemotherapy and at the time of progression. Additionally, blood samples will be drawn monthly and stored in the tissue biobank.

Interventions

Needle core biopsies of liver metastasis

No investigational products will be administered to subjects as part of this translational research study. A first-line chemotherapy regimen consisting of FOLFOX, XELOX or FOLFIRI +/- bevacizumab will be administered as per the standard of care at each treating institution. Needle core biopsies of liver metastasis will be collected and banked before the start of chemotherapy and at the time of progression. Additionally, blood samples will be drawn monthly and stored in the tissue biobank.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

1. Patients with a histologically confirmed diagnosis of colorectal cancer, with at least one liver metastasis site available for biopsy.

2. For patients with liver only disease, patients deemed not to be initially resectable

3. Scheduled to receive first-line chemotherapy (FOLFOX/bevacizumab or FOLFIRI/bevacizumab) for metastatic disease.

4. Measurable metastatic disease (at least one unidimensionally measurable lesion) present after planned biopsy of metastatic site(s).

5. ECOG 0, 1 or 2.

6. Life expectancy of 12 or more weeks.

7. Age > 18 years.

8. Able to adhere to the study visit schedule and other protocol requirements.

9. Normal coagulation profile (PT, PTT, INR).

Exclusion Criteria

1. Patients with initially resectable liver only metastases

2. Have received prior therapy for metastatic cancer. Prior adjuvant therapy is allowed.

3. Inadequate or unusable tissue as the only tissue available for biopsy.

4. Contraindication to any of the components of the the first-line chemotherapy regimen.

5. Known brain metastases or meningeal disease.

6. Female patients who are pregnant or breastfeeding.

7. Concurrent treatment with other anti-cancer therapy (palliative radiation is allowed but patients must have a metastatic site available for re-biopsy that has not been irradiated).

8. Abnormal coagulation profile, any anti-coagulant therapy.

9. Known infection with HIV.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Exactis Innovation

OTHER

Sponsor Role: collaborator

Terry Fox Research Institute

OTHER

Sponsor Role: collaborator

Fonds de la Recherche en Santé du Québec

OTHER_GOV

Sponsor Role: collaborator

Jewish General Hospital

OTHER

Sponsor Role: lead

Responsible Party

Gerald Batist

Principal Investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Gerald Batist, MD

Role: STUDY_DIRECTOR

Jewish General Hospital, Segal Cancer Center

Locations

University Hospital Leuven

Leuven, , Belgium

The Moncton Hospital

Moncton, New Brunswick, Canada

Dr. Georges L. Dumont University Hospital

Moncton, New Brunswick, Canada

Sunnybrook Health Sciences Centre

Toronto, Ontario, Canada

Mount Sinai Hospital

Toronto, Ontario, Canada

Hôpital Charles Lemoyne

Greenfield Park, Quebec, Canada

Hôpital Maisonneuve-Rosemont

Montreal, Quebec, Canada

Hôpital Notre-Dame

Montreal, Quebec, Canada

Hôpital Saint-Luc

Montreal, Quebec, Canada

Royal Victoria Hospital

Montreal, Quebec, Canada

Jewish General Hospital

Montreal, Quebec, Canada

St-Mary's Hospital

Montreal, Quebec, Canada

McGill University Health Centre

Montreal, Quebec, Canada

Hôpital Sacré-Coeur

Montreal, Quebec, Canada

Hôtel-Dieu du Québec

Québec, Quebec, Canada

Countries

Belgium; Canada

Other Identifiers

Q-CROC-01

Identifier Type: -

Identifier Source: org_study_id