Effects of Ethanol on Intestinal Permeability and Integrity

NCT ID: NCT00928733

Last Updated: 2014-11-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

17 participants

Study Classification

INTERVENTIONAL

Study Start Date

2009-09-30

Study Completion Date

2012-01-31

Brief Summary

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Alcohol consumption is a major health problem worldwide. It affects all systems of the body especially the gastrointestinal tract. Acute or chronic alcohol consumption has deleterious effects on the gastrointestinal mucosa vary from increased intestinal permeability, structural changes to sever destruction of the epithelial lining cells. Human data are still limited and most of the studies were performed in chronic alcohol abusers.

The investigators hypothesize that moderate alcohol drinking also may increase small intestinal permeability and contribute to the subsequent disruption of the tight junction complex. This study may provide more insight into the effects of moderate alcohol drinking on the small intestinal permeability.

Detailed Description

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Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Blinding Strategy

SINGLE

Investigators

Study Groups

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alcohol

Intraduodenal infusion of ethanol

Group Type EXPERIMENTAL

Gastroduodenoscopy- Intraduodenal intubation

Intervention Type DEVICE

20 g ethanol diluted up to 10% in tap water

Ethanol

Group Type OTHER

Gastroduodenoscopy- Intraduodenal intubation

Intervention Type DEVICE

20 g ethanol diluted up to 10% in tap water

Placebo

Intraduodenal infusion of tap water

Group Type EXPERIMENTAL

Gastroduodenoscopy- Intraduodenal intubation

Intervention Type DEVICE

20 g ethanol diluted up to 10% in tap water

Interventions

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Gastroduodenoscopy- Intraduodenal intubation

20 g ethanol diluted up to 10% in tap water

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Signed informed consent form.
* Male gender to avoid the gender-related differences in ethanol metabolism.
* Between 18-45 years to avoid age-related changes in ethanol metabolism39.
* Normal medical history and physical examination.
* Normal liver function tests (i.e. ALT, AST, and γGT) according to the reference values for normal ranges of the liver enzymes at the laboratory of clinical chemistry of the Maastricht University Medical Center.
* Caucasian ethnicity.
* BMI=18 - 30 kg/m2.

Exclusion Criteria

* History of gastro-intestinal disorders or abdominal surgery.
* History of alcohol abuse or current excessive alcohol consumption (\> 2 alcoholic beverages per day or \> 14 alcoholic beverages per week)40.
* Recent or chronic medications that may interact with ethanol metabolism or intestinal permeability i.e., NSAIDs, benzodiazepines and antidepressants.
* Smoking.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Top Institute Food and Nutrition

OTHER

Sponsor Role collaborator

Maastricht University Medical Center

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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A Masclee, MD PhD

Role: PRINCIPAL_INVESTIGATOR

Maastricht UMC

Locations

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Maastricht University Medical Center

Maastricht, Limburg, Netherlands

Site Status

Countries

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Netherlands

References

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Draper LR, Gyure LA, Hall JG, Robertson D. Effect of alcohol on the integrity of the intestinal epithelium. Gut. 1983 May;24(5):399-404. doi: 10.1136/gut.24.5.399.

Reference Type BACKGROUND
PMID: 6840613 (View on PubMed)

Hirsch S, Chaves G, Gotteland M, de la Maza P, Petermann M, Barrera P, Bunout D. [Intestinal permeability in alcoholic patients without liver damage]. Rev Med Chil. 1997 Jun;125(6):653-8. Spanish.

Reference Type BACKGROUND
PMID: 9515283 (View on PubMed)

Basuroy S, Sheth P, Mansbach CM, Rao RK. Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa: protection by EGF and L-glutamine. Am J Physiol Gastrointest Liver Physiol. 2005 Aug;289(2):G367-75. doi: 10.1152/ajpgi.00464.2004. Epub 2005 Feb 17.

Reference Type BACKGROUND
PMID: 15718285 (View on PubMed)

Elamin E, Masclee A, Troost F, Pieters HJ, Keszthelyi D, Aleksa K, Dekker J, Jonkers D. Ethanol impairs intestinal barrier function in humans through mitogen activated protein kinase signaling: a combined in vivo and in vitro approach. PLoS One. 2014 Sep 16;9(9):e107421. doi: 10.1371/journal.pone.0107421. eCollection 2014.

Reference Type RESULT
PMID: 25226407 (View on PubMed)

Related Links

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http://www.leakygut.co.uk/

Increased intestinal permeability

Other Identifiers

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09-3-026

Identifier Type: -

Identifier Source: org_study_id