Gene Expression Profile of Breast Cancer Samples After Vitamin D Supplementation

NCT ID: NCT00926315

Last Updated: 2012-11-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2007-07-31

Study Completion Date

2013-12-31

Brief Summary

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The purpose of this study is to evaluate whether calcitriol supplementation may reduce tumor cell proliferation and influence gene expression profile of breast cancer samples from post-menopausal patients.

Detailed Description

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Women affected by breast cancer present lower 1,25(OH)2D3 or 25(OH)D3 serum levels than unaffected ones. Calcitriol supplementation may be indicated to post-menopausal women to reduce bone loss. Vitamin D has antiproliferative effects in breast cancer cell lines and breast cancer xenografts.

Post-menopausal breast cancer patients will be prescribed calcitriol supplementation, in doses indicated to prevent osteoporosis, while they are submitted to biopsy, staging exams and have their breast surgery scheduled (approximately one month). Tumor dimension and proliferation rate (as determined by Ki67 expression), 25(OH)D3 and/or 1,25(OH)2D3 serum concentration, will be evaluated before and after calcitriol supplementation. Tumor gene expression will be evaluated in samples collected before and after supplementation to analyze the differential profile.

Conditions

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Breast Neoplasms Postmenopausal Disorder

Keywords

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Breast Neoplasms Calcitriol Postmenopausal Cell Proliferation Gene Expression Profiling

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Blinding Strategy

NONE

Study Groups

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calcitriol

calcitriol supplementation (0.25 mcg 2x/d)

Group Type EXPERIMENTAL

calcitriol

Intervention Type DRUG

Post menopausal patients will receive Calcitriol 0.50 mcg PO per day for 1 month.

Calcitriol

Intervention Type DRUG

calcitriol 0.25 mcg PO bid

Interventions

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calcitriol

Post menopausal patients will receive Calcitriol 0.50 mcg PO per day for 1 month.

Intervention Type DRUG

Calcitriol

calcitriol 0.25 mcg PO bid

Intervention Type DRUG

Other Intervention Names

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Rocaltrol Rocaltrol

Eligibility Criteria

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Inclusion Criteria

* Postmenopausal women
* Invasive breast carcinoma
* Clinical conditions for breast surgery
* No previous neoadjuvant treatment for breast cancer
* Agreement to take part in the study and sign the informed consent

Exclusion Criteria

* History of hypercalcemia or nephrolithiasis
* Current use of corticosteroids, vitamin D supplementation, HRT
* Previous chemotherapy, hormonotherapy or radiotherapy
* Parathyroid disease
* Absence of clinical condition to receive supplementation
Minimum Eligible Age

40 Years

Maximum Eligible Age

70 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Instituto Brasileiro de Controle do Cancer

OTHER

Sponsor Role collaborator

University of Sao Paulo General Hospital

OTHER

Sponsor Role lead

Responsible Party

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Eduardo Carneiro de Lyra

MD, PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Maria Aparecida A Koike Folgueira, MD,PhD

Role: STUDY_CHAIR

Faculdade de Medicina - Universidade de São Paulo

Eduardo Carneiro de Lyra, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Instituto Brasileiro de Controle do Cancer

Yuri N Urata, MSc

Role: PRINCIPAL_INVESTIGATOR

Faculdade de Medicina da Universidade de São Paulo

Maria Lucia H Katayama, PhD

Role: PRINCIPAL_INVESTIGATOR

Faculdade de Medicina da Universidade de São Paulo

Locations

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Instituto Brasileiro de Controle do Câncer - IBCC

São Paulo, São Paulo, Brazil

Site Status

Countries

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Brazil

References

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de Lyra EC, da Silva IA, Katayama ML, Brentani MM, Nonogaki S, Goes JC, Folgueira MA. 25(OH)D3 and 1,25(OH)2D3 serum concentration and breast tissue expression of 1alpha-hydroxylase, 24-hydroxylase and Vitamin D receptor in women with and without breast cancer. J Steroid Biochem Mol Biol. 2006 Aug;100(4-5):184-92. doi: 10.1016/j.jsbmb.2006.04.009. Epub 2006 Jul 7.

Reference Type BACKGROUND
PMID: 16828283 (View on PubMed)

Rozenchan PB, Folgueira MA, Katayama ML, Snitcovsky IM, Brentani MM. Ras activation is associated with vitamin D receptor mRNA instability in HC11 mammary cells. J Steroid Biochem Mol Biol. 2004 Sep;92(1-2):89-95. doi: 10.1016/j.jsbmb.2004.05.007.

Reference Type BACKGROUND
PMID: 15544934 (View on PubMed)

Katayama ML, Pasini FS, Folgueira MA, Snitcovsky IM, Brentani MM. Molecular targets of 1,25(OH)2D3 in HC11 normal mouse mammary cell line. J Steroid Biochem Mol Biol. 2003 Jan;84(1):57-69. doi: 10.1016/s0960-0760(03)00004-9.

Reference Type BACKGROUND
PMID: 12648525 (View on PubMed)

Bortman P, Folgueira MA, Katayama ML, Snitcovsky IM, Brentani MM. Antiproliferative effects of 1,25-dihydroxyvitamin D3 on breast cells: a mini review. Braz J Med Biol Res. 2002 Jan;35(1):1-9. doi: 10.1590/s0100-879x2002000100001.

Reference Type BACKGROUND
PMID: 11743608 (View on PubMed)

Folgueira MA, Federico MH, Katayama ML, Silva MR, Brentani MM. Expression of vitamin D receptor (VDR) in HL-60 cells is differentially regulated during the process of differentiation induced by phorbol ester, retinoic acid or interferon-gamma. J Steroid Biochem Mol Biol. 1998 Aug;66(4):193-201. doi: 10.1016/s0960-0760(98)00041-7.

Reference Type BACKGROUND
PMID: 9744516 (View on PubMed)

Janjoppi L, Katayama MH, Scorza FA, Folgueira MA, Brentani M, Pansani AP, Cavalheiro EA, Arida RM. Expression of vitamin D receptor mRNA in the hippocampal formation of rats submitted to a model of temporal lobe epilepsy induced by pilocarpine. Brain Res Bull. 2008 Jul 30;76(5):480-4. doi: 10.1016/j.brainresbull.2008.01.002. Epub 2008 Feb 5.

Reference Type BACKGROUND
PMID: 18534255 (View on PubMed)

Folgueira MA, Carraro DM, Brentani H, Patrao DF, Barbosa EM, Netto MM, Caldeira JR, Katayama ML, Soares FA, Oliveira CT, Reis LF, Kaiano JH, Camargo LP, Vencio RZ, Snitcovsky IM, Makdissi FB, e Silva PJ, Goes JC, Brentani MM. Gene expression profile associated with response to doxorubicin-based therapy in breast cancer. Clin Cancer Res. 2005 Oct 15;11(20):7434-43. doi: 10.1158/1078-0432.CCR-04-0548.

Reference Type BACKGROUND
PMID: 16243817 (View on PubMed)

Other Identifiers

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CAPPesq 626/06

Identifier Type: REGISTRY

Identifier Source: secondary_id

FAPESP 07/04799-2

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

IBCC 108/2006/07

Identifier Type: REGISTRY

Identifier Source: secondary_id

CAPPesq 0018/09

Identifier Type: REGISTRY

Identifier Source: secondary_id

FMUSPIBCCVD2009

Identifier Type: -

Identifier Source: org_study_id