Trial Outcomes & Findings for Vitamin D for the Treatment of Women With Polycystic Ovary Syndrome (PCOS) (NCT NCT00907153)

NCT ID: NCT00907153

Last Updated: 2017-12-19

Results Overview

Quantitative insulin sensitivity check index (QUICKI) is a validated measure of insulin sensitivity based on fasting insulin and glucose. Quantitative insulin sensitivity check index (QUICKI) = 1/\[log(I(0)) + log(G(0))\]).

Recruitment status

COMPLETED

Study phase

PHASE1/PHASE2

Target enrollment

36 participants

Primary outcome timeframe

Baseline and 12 weeks

Results posted on

2017-12-19

Participant Flow

36 patients were screened for eligibility between July 2009 and November 2010 at Medicine and Obstetrics and Gynecology clinics at an academic medical center in Hershey, PA.

28 of 36 participants were randomized. Of those not randomized, 3 did not meet inclusion criteria, 3 declined to participate, and 2 were not randomized for other reasons.

Participant milestones

Participant milestones
Measure
Vitamin D
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
Placebo: Placebo by mouth once daily for 12 weeks
Overall Study
STARTED
13
15
Overall Study
COMPLETED
11
11
Overall Study
NOT COMPLETED
2
4

Reasons for withdrawal

Reasons for withdrawal
Measure
Vitamin D
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
Placebo: Placebo by mouth once daily for 12 weeks
Overall Study
Lost to Follow-up
1
1
Overall Study
Withdrawal by Subject
1
3

Baseline Characteristics

Vitamin D for the Treatment of Women With Polycystic Ovary Syndrome (PCOS)

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Total
n=28 Participants
Total of all reporting groups
Age, Continuous
28.2 years
STANDARD_DEVIATION 5.2 • n=5 Participants
28.7 years
STANDARD_DEVIATION 5.6 • n=7 Participants
28.5 years
STANDARD_DEVIATION 5.3 • n=5 Participants
Sex: Female, Male
Female
13 Participants
n=5 Participants
15 Participants
n=7 Participants
28 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
White
10 Participants
n=5 Participants
14 Participants
n=7 Participants
24 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
1 Participants
n=5 Participants
0 Participants
n=7 Participants
1 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
1 Participants
n=5 Participants
1 Participants
n=7 Participants
2 Participants
n=5 Participants
Region of Enrollment
United States
13 participants
n=5 Participants
15 participants
n=7 Participants
28 participants
n=5 Participants
Body Mass Index (BMI)
37.20 kg/m2
STANDARD_DEVIATION 4.53 • n=5 Participants
35.09 kg/m2
STANDARD_DEVIATION 9.81 • n=7 Participants
36.1 kg/m2
STANDARD_DEVIATION 7.8 • n=5 Participants
Systolic blood pressure
117.46 mm Hg
STANDARD_DEVIATION 10.00 • n=5 Participants
113.91 mm Hg
STANDARD_DEVIATION 10.21 • n=7 Participants
115.56 mm Hg
STANDARD_DEVIATION 10.11 • n=5 Participants
Diastolic blood pressure
79.08 mm hg
STANDARD_DEVIATION 8.28 • n=5 Participants
74.88 mm hg
STANDARD_DEVIATION 7.72 • n=7 Participants
76.83 mm hg
STANDARD_DEVIATION 7.98 • n=5 Participants
25-hydroxyvitamin D
19.95 ng/mL
STANDARD_DEVIATION 9.47 • n=5 Participants
22.20 ng/mL
STANDARD_DEVIATION 6.86 • n=7 Participants
21.15 ng/mL
STANDARD_DEVIATION 8.10 • n=5 Participants
Vitamin D binding protein
30.46 mg/dL
STANDARD_DEVIATION 8.53 • n=5 Participants
31.95 mg/dL
STANDARD_DEVIATION 7.54 • n=7 Participants
31.34 mg/dL
STANDARD_DEVIATION 8.04 • n=5 Participants
Intact parathyroid hormone (i-PTH)
40.81 pg/mL
STANDARD_DEVIATION 27.34 • n=5 Participants
33.17 pg/mL
STANDARD_DEVIATION 17.73 • n=7 Participants
36.72 pg/mL
STANDARD_DEVIATION 22.19 • n=5 Participants
Fasting glucose
84.92 mg/dL
STANDARD_DEVIATION 9.46 • n=5 Participants
83.73 mg/dL
STANDARD_DEVIATION 9.33 • n=7 Participants
84.29 mg/dL
STANDARD_DEVIATION 9.23 • n=5 Participants
Fasting insulin
26.31 uU/mL
STANDARD_DEVIATION 9.60 • n=5 Participants
27.13 uU/mL
STANDARD_DEVIATION 15.79 • n=7 Participants
26.75 uU/mL
STANDARD_DEVIATION 13.05 • n=5 Participants
2-hour glucose
122.08 mg/dL
STANDARD_DEVIATION 36.29 • n=5 Participants
110.07 mg/dL
STANDARD_DEVIATION 23.68 • n=7 Participants
115.64 mg/dL
STANDARD_DEVIATION 30.22 • n=5 Participants
2-hour insulin
214.69 uU/mL
STANDARD_DEVIATION 146.41 • n=5 Participants
107.07 uU/mL
STANDARD_DEVIATION 55.20 • n=7 Participants
157.04 uU/mL
STANDARD_DEVIATION 118.72 • n=5 Participants
Insulin sensitivity index 0,120
55.55 mg·l^2/mmol·mU·min
STANDARD_DEVIATION 23.26 • n=5 Participants
63.56 mg·l^2/mmol·mU·min
STANDARD_DEVIATION 16.37 • n=7 Participants
59.84 mg·l^2/mmol·mU·min
STANDARD_DEVIATION 19.57 • n=5 Participants
Quantitative Insulin Sensitivity Check Index (QUICKI)
0.302 units on a scale
STANDARD_DEVIATION 0.014 • n=5 Participants
0.307 units on a scale
STANDARD_DEVIATION 0.029 • n=7 Participants
0.305 units on a scale
STANDARD_DEVIATION 0.022 • n=5 Participants
Homeostatic Model Assessment of Insulin Resistance (HOMA-IR)
5.47 units on a scale
STANDARD_DEVIATION 1.82 • n=5 Participants
5.80 units on a scale
STANDARD_DEVIATION 3.90 • n=7 Participants
5.65 units on a scale
STANDARD_DEVIATION 2.93 • n=5 Participants
Total cholesterol
172.00 mg/dL
STANDARD_DEVIATION 42.70 • n=5 Participants
184.27 mg/dL
STANDARD_DEVIATION 32.52 • n=7 Participants
178.57 mg/dL
STANDARD_DEVIATION 37.38 • n=5 Participants
HDL cholesterol
45.54 mg/dL
STANDARD_DEVIATION 17.60 • n=5 Participants
37.00 mg/dL
STANDARD_DEVIATION 10.83 • n=7 Participants
40.96 mg/dL
STANDARD_DEVIATION 14.74 • n=5 Participants
LDL cholesterol
98.62 mg/dL
STANDARD_DEVIATION 36.96 • n=5 Participants
117.33 mg/dL
STANDARD_DEVIATION 28.56 • n=7 Participants
108.64 mg/dL
STANDARD_DEVIATION 33.47 • n=5 Participants
Triglyecrides
139.08 mg/dL
STANDARD_DEVIATION 71.61 • n=5 Participants
149.47 mg/dL
STANDARD_DEVIATION 85.46 • n=7 Participants
144.64 mg/dL
STANDARD_DEVIATION 78.07 • n=5 Participants
High sensitive C-reactive protein (hsCRP)
7.95 mg/L
STANDARD_DEVIATION 5.24 • n=5 Participants
4.42 mg/L
STANDARD_DEVIATION 4.34 • n=7 Participants
6.1 mg/L
STANDARD_DEVIATION 5.0 • n=5 Participants
Total testosterone
54.23 ng/dL
STANDARD_DEVIATION 28.16 • n=5 Participants
41.27 ng/dL
STANDARD_DEVIATION 17.29 • n=7 Participants
48.48 ng/dL
STANDARD_DEVIATION 23.03 • n=5 Participants
Free testosterone
19.08 ng/dL
STANDARD_DEVIATION 15.79 • n=5 Participants
14.00 ng/dL
STANDARD_DEVIATION 10.88 • n=7 Participants
16.89 ng/dL
STANDARD_DEVIATION 13.33 • n=5 Participants

PRIMARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Quantitative insulin sensitivity check index (QUICKI) is a validated measure of insulin sensitivity based on fasting insulin and glucose. Quantitative insulin sensitivity check index (QUICKI) = 1/\[log(I(0)) + log(G(0))\]).

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Quantitative Insulin Sensitivity Check Index (QUICKI)
-0.008 units on a scale
Interval -0.02 to 0.004
0.009 units on a scale
Interval -0.003 to 0.021

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

High sensitive C-reactive protein (hsCRP) was assessed as a measure of inflammation.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean High Sensitive C-reactive Protein (hsCRP)
0.90 mg/L
Interval -2.46 to 4.27
2.04 mg/L
Interval -1.37 to 5.45

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Blood pressure was measured in the right arm in the sitting position after a 15-minute rest.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Systolic Blood Pressure
0.64 mm Hg
Interval -7.13 to 8.42
4.29 mm Hg
Interval -3.15 to 11.74

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Blood pressure was measured in the right arm in the sitting position after a 15-minute rest.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Diastolic Blood Pressure
-0.91 mm Hg
Interval -4.96 to 3.14
5.60 mm Hg
Interval 1.69 to 9.52

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Glucose was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Fasting Glucose
-0.70 mg/dL
Interval -7.96 to 6.56
-6.98 mg/dL
Interval -14.31 to 0.34

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Insulin was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Fasting Insulin
13.04 uU/mL
Interval -3.96 to 30.05
-0.80 uU/mL
Interval -18.03 to 16.43

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Participants underwent a 75-gram oral glucose tolerance test, in which blood samples for glucose and insulin were obtained at 0 and 2 hours and used to calculate the insulin sensitivity index (ISI 0,120).

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean 2-hour Glucose
-11.66 mg/dL
Interval -33.22 to 9.89
1.14 mg/dL
Interval -20.1 to 22.38

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Participants underwent a 75-gram oral glucose tolerance test, in which blood samples for glucose and insulin were obtained at 0 and 2 hours and used to calculate the insulin sensitivity index (ISI 0,120).

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean 2-hour Insulin
-62.02 uU/mL
Interval -124.5 to 0.49
13.06 uU/mL
Interval -47.94 to 74.06

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Participants underwent a 75-g oral glucose tolerance test, in which blood samples for glucose and insulin were obtained at 0 and 120 minutes and used to calculate the insulin sensitivity index (ISI0,120). The ISI 0,120 = the glucose uptake rate divided by the mean plasma glucose divided by the log(mean serum insulin).

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Insulin Sensitivity Index (ISI 0,120)
6.67 mg·l^2/mmol·mU·min
Interval -16.87 to 30.21
5.94 mg·l^2/mmol·mU·min
Interval -17.77 to 29.65

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) is a validated measure of insulin resistance based on fasting insulin and glucose. HOMA-IR is calculated as the product of fasting glucose and insulin divided by 22.5.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Homeostatic Model Assessment of Insulin Resistance (HOMA-IR)
2.57 units on a scale
Interval -0.9 to 6.03
-0.51 units on a scale
Interval -4.01 to 2.99

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Lipid profile was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Total Cholesterol
-1.69 mg/dL
Interval -19.04 to 15.66
-1.80 mg/dL
Interval -19.55 to 15.95

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Lipid profile was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean HDL Cholesterol
-0.70 mg/dL
Interval -6.52 to 5.12
1.23 mg/dL
Interval -4.68 to 7.14

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Lipid profile was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean LDL Cholesterol
-0.12 mg/dL
Interval -14.7 to 14.46
-0.40 mg/dL
Interval -15.24 to 14.45

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Lipid profile was assessed after 12 hours of fasting.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Triglycerides
-2.21 mg/dL
Interval -34.11 to 29.69
-12.44 mg/dL
Interval -44.68 to 19.8

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Total and free testosterone levels were assessed from blood samples to evaluate effects on hyperandrogenemia in PCOS.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Total Testosterone
3.01 ng/dL
Interval -8.05 to 14.06
10.16 ng/dL
Interval -1.16 to 21.47

SECONDARY outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Total and free testosterone levels were assessed from blood samples to evaluate effects on hyperandrogenemia in PCOS.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Free Testosterone
2.67 ng/dL
Interval -1.29 to 6.63
5.82 ng/dL
Interval 1.82 to 9.82

OTHER_PRE_SPECIFIED outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Total 25-hydroxyvitamin D was assayed by the Immunodiagnostic Systems radioimmunoassay.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean 25-hydroxyvitamin D
45.63 ng/mL
Interval 33.5 to 57.76
1.32 ng/mL
Interval -10.93 to 13.58

OTHER_PRE_SPECIFIED outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Vitamin D binding protein levels were assessed as it has been linked with insulin resistance and type 2 diabetes.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Vitamin D Binding Protein
1.01 mg/dL
Interval -1.6 to 3.62
-0.55 mg/dL
Interval -3.14 to 2.04

OTHER_PRE_SPECIFIED outcome

Timeframe: Baseline and 12 weeks

Population: All analyses were by intention to treat. All randomized participants received and ingested the treatment that they were randomly assigned to.

Intact parathyroid hormone levels were assessed as they have been linked with obesity and insulin resistance.

Outcome measures

Outcome measures
Measure
Vitamin D
n=13 Participants
Vitamin D: Vitamin D 300 mcg by mouth once daily for 12 weeks
Placebo
n=15 Participants
Placebo: Placebo by mouth once daily for 12 weeks
Change From Baseline in Mean Intact Parathyroid Hormone (i-PTH)
-24.28 pg/mL
Interval -39.8 to -8.76
-16.45 pg/mL
Interval -31.4 to -1.49

Adverse Events

Vitamin D

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Placebo

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Dr. Nazia Raja-Khan

Penn State Hershey Medical Center

Phone: 717-531-8395

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place