Trial Outcomes & Findings for Eltrombopag and the Bcl-extra-large (xL) Pathway in Idiopathic Thrombocytopenic Purpura (ITP) (NCT NCT00902018)

NCT ID: NCT00902018

Last Updated: 2019-03-18

Results Overview

number of patients in whom Platelet Counts measured on days 8 and 15 after eltrombopag treatment increase to \> 50,000/uL counts on other days are just used to be sure the ones on days 8 and 15 are reasonably accurate and representative

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

20 participants

Primary outcome timeframe

platelet counts on days 8 and 15

Results posted on

2019-03-18

Participant Flow

patients with persistent or chronic ITP willing to undergo washout period and then multiple visits (7) in 2 weeks period including 3 larger blood draws

Participant milestones

Participant milestones
Measure
Eltrombopag
Promacta (eltrombopag): Subjects will be treated with eltrombopag 75 mg once daily. Patients will be monitored 3 times a week for the first 2 weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for 3-4 months. first blood draw before taking eltrombopag
Eltombopag Then Romiplostim
patients who receive eltrombopag and then are treated with romiplostim (nplate) to ascertain parallelism between the two agents
Healthy Volunteer
one blood draw only
Overall Study
STARTED
7
3
10
Overall Study
COMPLETED
7
3
10
Overall Study
NOT COMPLETED
0
0
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

Eltrombopag and the Bcl-extra-large (xL) Pathway in Idiopathic Thrombocytopenic Purpura (ITP)

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Eltrombopag Arm
n=7 Participants
Participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Eltrombopag Receiving Romiplostim
n=3 Participants
Three Participants who had been on eltrombopag in the past will undergo a washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks.
Healthy Volunteers
n=10 Participants
single blood draw in healthy volunteers (controls)
Total
n=20 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
0 Participants
n=4 Participants
Age, Categorical
Between 18 and 65 years
3 Participants
n=5 Participants
2 Participants
n=7 Participants
10 Participants
n=5 Participants
15 Participants
n=4 Participants
Age, Categorical
>=65 years
4 Participants
n=5 Participants
1 Participants
n=7 Participants
0 Participants
n=5 Participants
5 Participants
n=4 Participants
Sex: Female, Male
Female
3 Participants
n=5 Participants
2 Participants
n=7 Participants
8 Participants
n=5 Participants
13 Participants
n=4 Participants
Sex: Female, Male
Male
4 Participants
n=5 Participants
1 Participants
n=7 Participants
2 Participants
n=5 Participants
7 Participants
n=4 Participants
Region of Enrollment
United States
7 participants
n=5 Participants
3 participants
n=7 Participants
10 participants
n=5 Participants
20 participants
n=4 Participants

PRIMARY outcome

Timeframe: platelet counts on days 8 and 15

Population: 0 participants were analyzed in the healthy control arm because healthy controls did not have any blood draws on day 8 and 15 or receive the intervention and hence data was not collected for these participants

number of patients in whom Platelet Counts measured on days 8 and 15 after eltrombopag treatment increase to \> 50,000/uL counts on other days are just used to be sure the ones on days 8 and 15 are reasonably accurate and representative

Outcome measures

Outcome measures
Measure
Eltrombopag Arm
n=7 Participants
Participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Eltrombopag Then Romiplostim
n=3 Participants
Three Participants from Eltrombopag arm will undergo second washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks exactly like when they received Eltrombopag.
Healthy Controls
10 normal volunteers for a single blood draw
Number of Patients for Whom Eltrombopag Increases the Platelet Count to > 50,000/uL
pts with platelet counts > 50,000/uL on day 8
6 participants
3 participants
Number of Patients for Whom Eltrombopag Increases the Platelet Count to > 50,000/uL
Pts with platelet counts > 50,000/uL on day 15
6 participants
3 participants

PRIMARY outcome

Timeframe: platelet counts on days 8 and 15

number of participants in whom platelet counts measured on day 8 and day 15 after treatment(s) with romiplostim 10 micrograms/kg on days 1 and 8

Outcome measures

Outcome measures
Measure
Eltrombopag Arm
n=3 Participants
Participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Eltrombopag Then Romiplostim
Three Participants from Eltrombopag arm will undergo second washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks exactly like when they received Eltrombopag.
Healthy Controls
10 normal volunteers for a single blood draw
Number of Patients Who Received Romiplostim and Increased Their Platelet Counts to > 50,000/uL
plt cts > 50,000/uL on day 8
3 participants
Number of Patients Who Received Romiplostim and Increased Their Platelet Counts to > 50,000/uL
plt cts > 50,000/uL on day 15
3 participants

SECONDARY outcome

Timeframe: on days 8 and 15

Population: 0 participants were analyzed in the healthy controls arm because healthy controls did not have any blood draw on day 8 and 15 or receive the intervention and hence data was not collected for these participants

To assess the safety of eltrombopag, in particular the number of patients with serious adverse events and/or abnormal liver tests reaching a level of more than twice the upper limit of normal for the test these outcomes were assessed periodically for liver tests but other SAEs were not systematically assessed but only with complaints or events

Outcome measures

Outcome measures
Measure
Eltrombopag Arm
n=10 Participants
Participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Eltrombopag Then Romiplostim
n=3 Participants
Three Participants from Eltrombopag arm will undergo second washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks exactly like when they received Eltrombopag.
Healthy Controls
10 normal volunteers for a single blood draw
How Many Patients Developed SAEs and/or Abnormal Liver Tests to a Level > 2 Times the Upper Limit of Normal
number of patients with Abnl LFTs on day 8
1 participants
0 participants
How Many Patients Developed SAEs and/or Abnormal Liver Tests to a Level > 2 Times the Upper Limit of Normal
number of patients Abnl LFTs on day 15
1 participants
0 participants
How Many Patients Developed SAEs and/or Abnormal Liver Tests to a Level > 2 Times the Upper Limit of Normal
number of patients with an SAE on day 8
0 participants
0 participants
How Many Patients Developed SAEs and/or Abnormal Liver Tests to a Level > 2 Times the Upper Limit of Normal
number of patients with an SAE on day 15
0 participants
0 participants

OTHER_PRE_SPECIFIED outcome

Timeframe: testing on days 8 and 15

Population: 0 participants were analyzed in the healthy controls arm because healthy controls did not have any blood draw on day 8 and 15 or receive the intervention and hence data was not collected for these participants

Samples were drawn weekly for 2 weeks on days 1, 8, and 15 for the treatment arms and day 1 for the healthy control group. Platelet samples were exposed to small molecule Bcl-xL inhibitor, ABT-737 ex-vivo to explore resistance to apoptosis by determining the half maximal inhibitory concentration (IC50) which was measured for each weekly sample drawn. If the half maximal concentration of ABT737 was increased this meant increased resistance to apoptosis. The AKT pathway intermediates were measured since these would indicate the mechanism of the platelet resistance to apoptosis so the two sets of measures confirm each other the AIPF is a measure of how many new platelets are made and the large platelets are similar to that

Outcome measures

Outcome measures
Measure
Eltrombopag Arm
n=7 Participants
Participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Eltrombopag Then Romiplostim
n=3 Participants
Three Participants from Eltrombopag arm will undergo second washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks exactly like when they received Eltrombopag.
Healthy Controls
10 normal volunteers for a single blood draw
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
platelet apoptosis increase on day 8
7 Participants
3 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
platelet apoptosis increase on day 15
2 Participants
0 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in intracellular AKT intermediates day 8
7 Participants
3 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in intracellular AKT intermediates day 15
0 Participants
1 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in A-IPF day 8
7 Participants
3 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in A-IPF day 15
7 Participants
3 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in large platelets day 8
7 Participants
3 Participants
Change From Baseline After Eltrombopag Treatment of Platelet Parameters
increase in large platelets day 15
7 Participants
3 Participants

Adverse Events

Eltrombopag

Serious events: 0 serious events
Other events: 1 other events
Deaths: 0 deaths

Romiplostim

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Healthy Volunteers

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Eltrombopag
n=10 participants at risk
Ten participants will be treated with eltrombopag 75 mg once daily. Participants will be monitored three times a week for the first two weeks, and then monitored as clinically indicated as they continue eltrombopag dosing for four months.
Romiplostim
n=3 participants at risk
Three Participants who had been on eltrombopag will undergo a washout period and will be treated with Romiplostim 10 ug/kg/weekly for 2 weeks. Participants on Romiplostim will be monitored three times a week for two weeks.
Healthy Volunteers
n=10 participants at risk
single blood draw in healthy controls/volunteers
Hepatobiliary disorders
increase in LFTs
10.0%
1/10 • Number of events 1 • data for investigation collected over 2 weeks in all patients: 7 with eltrombopag alone and 3 with eltrombopag first and, then after a washout period, romiplostim later this would be 10 for eltrombopag and 3 for romiplostim additional medication provided as incentive to patients to enter the study there was a single blood draw without incident in the 10 healthy volunteers
patients were asked for concomitant meds and Adverse Events on every visit
0.00%
0/3 • data for investigation collected over 2 weeks in all patients: 7 with eltrombopag alone and 3 with eltrombopag first and, then after a washout period, romiplostim later this would be 10 for eltrombopag and 3 for romiplostim additional medication provided as incentive to patients to enter the study there was a single blood draw without incident in the 10 healthy volunteers
patients were asked for concomitant meds and Adverse Events on every visit
0.00%
0/10 • data for investigation collected over 2 weeks in all patients: 7 with eltrombopag alone and 3 with eltrombopag first and, then after a washout period, romiplostim later this would be 10 for eltrombopag and 3 for romiplostim additional medication provided as incentive to patients to enter the study there was a single blood draw without incident in the 10 healthy volunteers
patients were asked for concomitant meds and Adverse Events on every visit

Additional Information

Dr James Bussel

Weill Cornell Medical College

Phone: 22-746-3474

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place