Study of Biomarkers in Blood & Tissue Samples From Patients With Colorectal Cancer or Polyps & Patients Without Polyps

NCT ID: NCT00898378

Last Updated: 2014-09-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

551 participants

Study Classification

OBSERVATIONAL

Study Start Date

2009-01-31

Study Completion Date

2014-03-31

Brief Summary

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RATIONALE: Studying samples of blood and tissue in the laboratory from patients with cancer, patients with colorectal polyps and from patients without polyps may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is looking at biomarkers in blood and tissue samples from patients with colorectal cancer or colorectal polyps and from patients without polyps (healthy volunteers).

Detailed Description

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OBJECTIVES:

Primary

* Perform metabolomic, lipidomic, glycoproteomic, proteomic, and genomic (OMIC) profiling using blood and tissue samples from patients with colorectal cancer (CRC) or colorectal adenomatous polyps and from patients without polyps.
* Create an OMIC profile to predict the risk of CRC based on differences observed between patients with CRC, patients with colorectal adenomatous polyps, and patients without polyps.

Secondary

* Create an OMIC profile to predict response and toxicity to specific chemotherapies, biological therapies, and radiotherapy for CRC.
* Utilize a novel knowledge discovery tool (BioMap) based on literature mining and, in the future, utilize the results of the OMIC analyses to identify interactive molecular pathways that underlie the development and progression of CRC.

OUTLINE: Patients with locally advanced or metastatic colorectal cancer are stratified according to treatment (first-line chemotherapy with fluorouracil \[5-FU\]/oxaliplatin or 5-FU/irinotecan vs second- or third-line chemotherapy with irinotecan only vs biological therapy with bevacizumab vs biological therapy with cetuximab vs radiotherapy).

Blood and tissue samples are collected periodically for laboratory studies. Samples are analyzed for metabolomics by nuclear magnetic resonance, gas chromatography, liquid chromatography, and mass spectrometry; lipidomics, proteomics, and glycoproteomics by liquid chromatography and mass spectrometry; and genomics (single nucleotide polymorphism biomarkers) by PCR. Vitamin D status is also assessed.

Patients complete diet-history and lifestyle questionnaires at baseline and once a year for 2 years. Healthy volunteers complete these questionnaires only at baseline.

After completion of study, patients are followed every 3 months for 2 years, every 6 months for 3 years, and then annually thereafter. Healthy volunteers are not followed after study completion.

Conditions

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Colorectal Cancer Healthy, no Evidence of Disease Precancerous Condition

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Colorectal Cancer Patients

Patients with stages I/II, III and IV colorectal cancer

gene expression analysis

Intervention Type GENETIC

gene expression analysis

polymerase chain reaction

Intervention Type GENETIC

polymerase chain reaction

polymorphism analysis

Intervention Type GENETIC

polymorphism analysis

protein expression analysis

Intervention Type GENETIC

protein expression analysis

proteomic profiling

Intervention Type GENETIC

proteomic profiling

gas chromatography

Intervention Type OTHER

gas chromatography

laboratory biomarker analysis

Intervention Type OTHER

laboratory biomarker analysis

liquid chromatography

Intervention Type OTHER

liquid chromatography

mass spectrometry

Intervention Type OTHER

mass spectrometry

questionnaire administration

Intervention Type OTHER

questionnaire administration

Colorectal Polyps Patients

Patients with adenomatous polyp(s) after colonoscopy.

gene expression analysis

Intervention Type GENETIC

gene expression analysis

polymerase chain reaction

Intervention Type GENETIC

polymerase chain reaction

polymorphism analysis

Intervention Type GENETIC

polymorphism analysis

protein expression analysis

Intervention Type GENETIC

protein expression analysis

proteomic profiling

Intervention Type GENETIC

proteomic profiling

gas chromatography

Intervention Type OTHER

gas chromatography

laboratory biomarker analysis

Intervention Type OTHER

laboratory biomarker analysis

liquid chromatography

Intervention Type OTHER

liquid chromatography

mass spectrometry

Intervention Type OTHER

mass spectrometry

questionnaire administration

Intervention Type OTHER

questionnaire administration

Healthy Controls

No abnormalities after colonoscopy.

gene expression analysis

Intervention Type GENETIC

gene expression analysis

polymerase chain reaction

Intervention Type GENETIC

polymerase chain reaction

polymorphism analysis

Intervention Type GENETIC

polymorphism analysis

protein expression analysis

Intervention Type GENETIC

protein expression analysis

proteomic profiling

Intervention Type GENETIC

proteomic profiling

gas chromatography

Intervention Type OTHER

gas chromatography

laboratory biomarker analysis

Intervention Type OTHER

laboratory biomarker analysis

liquid chromatography

Intervention Type OTHER

liquid chromatography

mass spectrometry

Intervention Type OTHER

mass spectrometry

questionnaire administration

Intervention Type OTHER

questionnaire administration

Interventions

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gene expression analysis

gene expression analysis

Intervention Type GENETIC

polymerase chain reaction

polymerase chain reaction

Intervention Type GENETIC

polymorphism analysis

polymorphism analysis

Intervention Type GENETIC

protein expression analysis

protein expression analysis

Intervention Type GENETIC

proteomic profiling

proteomic profiling

Intervention Type GENETIC

gas chromatography

gas chromatography

Intervention Type OTHER

laboratory biomarker analysis

laboratory biomarker analysis

Intervention Type OTHER

liquid chromatography

liquid chromatography

Intervention Type OTHER

mass spectrometry

mass spectrometry

Intervention Type OTHER

questionnaire administration

questionnaire administration

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

PATIENT CHARACTERISTICS:

* Not pregnant
* Able to undergo an 8-hour overnight fast prior to metabolomic testing
* Able to attend follow-up or treatment visits for up to 24 months for collection of blood samples
* No prior or concurrent invasive cancer other than CRC (for patients with CRC)
* No prior invasive cancer and no first-degree relative with a known history of CRC (for healthy volunteers and patients with colorectal polyps)

PRIOR CONCURRENT THERAPY:

* See Disease Characteristics
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Indiana University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Patrick J Loehrer, MD

Role: PRINCIPAL_INVESTIGATOR

Indiana University Melvin and Bren Simon Cancer Center

Locations

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Indiana University Melvin and Bren Simon Cancer Center

Indianapolis, Indiana, United States

Site Status

Countries

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United States

Other Identifiers

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P30CA082709

Identifier Type: NIH

Identifier Source: secondary_id

View Link

IUCRO-0221

Identifier Type: OTHER

Identifier Source: secondary_id

0808-24; IUCRO-0221

Identifier Type: -

Identifier Source: org_study_id

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