Trial Outcomes & Findings for Immunogenicity and Safety Study of rThrombin in Surgical Hemostasis (NCT NCT00813904)

NCT ID: NCT00813904

Last Updated: 2011-12-08

Results Overview

Immunogenicity of rThrombin product was evaluated using an enzyme-linked immunosorbent assay for detection of antirThrombin product antibody and a neutralizing antibody assay to characterize the potential of antibodies to rThrombin product to neutralize the activity of human plasma-derived thrombin.

Recruitment status

COMPLETED

Study phase

PHASE4

Target enrollment

31 participants

Primary outcome timeframe

At baseline and Day 29

Results posted on

2011-12-08

Participant Flow

Of 31 participants enrolled, all received treatment with recombinant thrombin (rThrombin).

Participant milestones

Participant milestones
Measure
rThrombin, 1000 IU/mL
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Overall Study
STARTED
31
Overall Study
COMPLETED
30
Overall Study
NOT COMPLETED
1

Reasons for withdrawal

Reasons for withdrawal
Measure
rThrombin, 1000 IU/mL
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Overall Study
Death
1

Baseline Characteristics

Immunogenicity and Safety Study of rThrombin in Surgical Hemostasis

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
rThrombin, 1000 IU/mL
n=31 Participants
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Age, Customized
59.5 Years
STANDARD_DEVIATION 12.3 • n=5 Participants
Age, Customized
Median
61.0 Years
n=5 Participants
Sex: Female, Male
Female
12 Participants
n=5 Participants
Sex: Female, Male
Male
19 Participants
n=5 Participants
Type of surgery
Arterial reconstruction/peripheral arterial bypass
4 Participants
n=5 Participants
Type of surgery
Arteriovenous vascular access procedure
3 Participants
n=5 Participants
Type of surgery
Other
1 Participants
n=5 Participants
Type of surgery
Spinal
23 Participants
n=5 Participants

PRIMARY outcome

Timeframe: At baseline and Day 29

Population: Participants who received treatment with rThrombin and had data available from both baseline and Day 29 antibody assessments.

Immunogenicity of rThrombin product was evaluated using an enzyme-linked immunosorbent assay for detection of antirThrombin product antibody and a neutralizing antibody assay to characterize the potential of antibodies to rThrombin product to neutralize the activity of human plasma-derived thrombin.

Outcome measures

Outcome measures
Measure
rThrombin, 1000 IU/mL
n=30 Participants
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Number or Participants With Antirecombinant Thrombin (rThrombin) Product Antibody at Baseline and Day 29
Baseline
0 Participants
Number or Participants With Antirecombinant Thrombin (rThrombin) Product Antibody at Baseline and Day 29
Day 29
0 Participants

SECONDARY outcome

Timeframe: Baseline through Day 29, continuously

Population: All participants who received treatment with rThrombin.

An SAE is any unfavorable medical event that at any dose results in death, persistent or significant disability/incapacity, or drug dependency or abuse; is life-threatening, an important medical event, or a congenital anomaly/birth defect; or requires or prolongs hospitalization. An AE is any new unfavorable symptom, sign, or disease or worsening of a preexisting condition that does not necessarily have a causal relationship with treatment. Treatment-related=possibly, probably, or certainly related to and of unknown relationship to study treatment.

Outcome measures

Outcome measures
Measure
rThrombin, 1000 IU/mL
n=31 Participants
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
AEs
29 Participants
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
Death
1 Participants
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
SAEs
6 Participants
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
Treatment-related SAEs
0 Participants
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
Treatment-related AEs
0 Participants
Number of Participants With Death as Outcome, Serious Adverse Events (SAEs), Treatment-related SAEs, Adverse Events (AEs), Treatment-related Adverse Events, and AEs Leading to Discontinuation
AEs leading to discontinuation
0 Participants

SECONDARY outcome

Timeframe: Baseline to Day 29, continuously

Population: All participants who received treatment with rThrombin.

An AE is any new unfavorable symptom, sign, or disease or worsening of a preexisting condition that does not necessarily have a causal relationship with treatment. AE severity was assessed using Common Terminology Criteria for Adverse Events, Version 13.0: Grade 1=mild; Grade 2=moderate; Grade 3=severe; Grade 4=life-threatening; Grade 5=fatal.

Outcome measures

Outcome measures
Measure
rThrombin, 1000 IU/mL
n=31 Participants
At least 1 application of reconstituted rThrombin, 1000 IU/mL, applied topically directly to the bleeding site.
Number of Participants With AEs by Maximum Severity
Mild
1 Participants
Number of Participants With AEs by Maximum Severity
Moderate
18 Participants
Number of Participants With AEs by Maximum Severity
Severe
9 Participants
Number of Participants With AEs by Maximum Severity
Life-threatening
0 Participants
Number of Participants With AEs by Maximum Severity
Fatal
1 Participants

Adverse Events

TOTAL

Serious events: 6 serious events
Other events: 29 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
TOTAL
n=31 participants at risk
General disorders
CHEST PAIN
3.2%
1/31
Infections and infestations
OSTEOMYELITIS
3.2%
1/31
Infections and infestations
SEPSIS
3.2%
1/31
Injury, poisoning and procedural complications
OVERDOSE
3.2%
1/31
Injury, poisoning and procedural complications
PROCEDURAL HYPOTENSION
3.2%
1/31
Nervous system disorders
SYNCOPE
3.2%
1/31
Renal and urinary disorders
RENAL FAILURE
3.2%
1/31
Renal and urinary disorders
RENAL FAILURE CHRONIC
3.2%
1/31
Vascular disorders
ARTERIAL THROMBOSIS LIMB
3.2%
1/31
Vascular disorders
HYPOTENSION
3.2%
1/31
Vascular disorders
PERIPHERAL ISCHAEMIA
3.2%
1/31

Other adverse events

Other adverse events
Measure
TOTAL
n=31 participants at risk
Blood and lymphatic system disorders
ANAEMIA
6.5%
2/31
Cardiac disorders
TACHYCARDIA
19.4%
6/31
Gastrointestinal disorders
CONSTIPATION
25.8%
8/31
Gastrointestinal disorders
NAUSEA
25.8%
8/31
Gastrointestinal disorders
VOMITING
6.5%
2/31
General disorders
PYREXIA
9.7%
3/31
Injury, poisoning and procedural complications
ANAEMIA POSTOPERATIVE
19.4%
6/31
Injury, poisoning and procedural complications
INCISION SITE PAIN
16.1%
5/31
Injury, poisoning and procedural complications
PROCEDURAL HYPOTENSION
9.7%
3/31
Injury, poisoning and procedural complications
PROCEDURAL PAIN
74.2%
23/31
Metabolism and nutrition disorders
HYPOKALAEMIA
6.5%
2/31
Musculoskeletal and connective tissue disorders
MUSCLE SPASMS
19.4%
6/31
Musculoskeletal and connective tissue disorders
PAIN IN EXTREMITY
6.5%
2/31
Nervous system disorders
HYPOAESTHESIA
6.5%
2/31
Nervous system disorders
RADICULOPATHY
6.5%
2/31
Psychiatric disorders
INSOMNIA
12.9%
4/31
Respiratory, thoracic and mediastinal disorders
DYSPHONIA
6.5%
2/31
Respiratory, thoracic and mediastinal disorders
HYPOXIA
6.5%
2/31
Respiratory, thoracic and mediastinal disorders
OROPHARYNGEAL PAIN
6.5%
2/31
Skin and subcutaneous tissue disorders
PRURITUS
12.9%
4/31
Vascular disorders
HYPERTENSION
6.5%
2/31

Additional Information

John Pribble, Vice President, Medical Affairs; Scot Maxon, Scientific Information

ZymoGenetics, a Bristol-Myers Squibb company

Phone: (206) 428-2756; (206) 434-3365

Results disclosure agreements

  • Principal investigator is a sponsor employee ZymoGenetics (ZG) agreements vary by trial, but each states that presentation/publication (p/p) cannot disclose Confidential Information (CI), excluding trial results; p/p may not begin until the earlier of 18 months after study end, ZG/lead investigator publishes, or ZG notification that multicenter publication is not planned; ZG has specified time for draft review before submission/presentation and may delay or modify content, require CI removal, and delay p/p for patent application filing.
  • Publication restrictions are in place

Restriction type: OTHER