Trial Outcomes & Findings for NAC Phase IIB: A Multi-Center, Phase IIB, Randomized, Placebo-controlled, Double-Blind Study Of The Effects Of N-Acetylcysteine On Redox Changes and Lung Inflammation In Cystic Fibrosis Patients (NCT NCT00809094)

NCT ID: NCT00809094

Last Updated: 2013-04-22

Results Overview

(change in log10 HNE in the active treatment group) - (change in log10 HNE in the placebo group)

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

70 participants

Primary outcome timeframe

From enrollment to end of the 24-week trial

Results posted on

2013-04-22

Participant Flow

Recruitment began 11/4/2008. Recruitment process was staggered: Stanford cohort first to focus on safety data related to potential pulmonary hypertension. After half of the Stanford cohort reached the 8-week time point, the DSMC evaluated PH safety data. The other sites then began enrollment. Final subject enrolled on 2/2011.

Participant milestones

Participant milestones
Measure
Study Drug
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Overall Study
STARTED
36
34
Overall Study
COMPLETED
36
33
Overall Study
NOT COMPLETED
0
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Study Drug
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Overall Study
Lost to Follow-up
0
1

Baseline Characteristics

NAC Phase IIB: A Multi-Center, Phase IIB, Randomized, Placebo-controlled, Double-Blind Study Of The Effects Of N-Acetylcysteine On Redox Changes and Lung Inflammation In Cystic Fibrosis Patients

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Study Drug
n=36 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=34 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Total
n=70 Participants
Total of all reporting groups
Age, Categorical
<=18 years
9 Participants
n=5 Participants
10 Participants
n=7 Participants
19 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
27 Participants
n=5 Participants
24 Participants
n=7 Participants
51 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Sex: Female, Male
Female
16 Participants
n=5 Participants
19 Participants
n=7 Participants
35 Participants
n=5 Participants
Sex: Female, Male
Male
20 Participants
n=5 Participants
15 Participants
n=7 Participants
35 Participants
n=5 Participants

PRIMARY outcome

Timeframe: From enrollment to end of the 24-week trial

Population: The primary endpoint analyses described uses the subset of the ITT population with complete case data. 70 subjects randomized, 65 completed the Week 12 visit and 62 completed the study. There were 6 withdrawals in the NAC group and 2 in Placebo. Five out of 6 subjects in the NAC group withdrew due to subject decision. Missing data was not imputed.

(change in log10 HNE in the active treatment group) - (change in log10 HNE in the placebo group)

Outcome measures

Outcome measures
Measure
Study Drug
n=26 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=30 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Change in the Logarithm of the Level of Human Neutrophil Elastase (HNE) Activity Measured in Sputum
0.03 log10 mcg/mL
Interval -1.12 to 1.38
-0.17 log10 mcg/mL
Interval -1.2 to 0.67

SECONDARY outcome

Timeframe: From enrollment to the end of the 24-week trial

Population: Out of 70 subjects randomized, 65 completed the Week 12 visit and 62 completed the study. There were 6 withdrawals in the NAC group and 2 in Placebo. Five out of 6 subjects in the NAC group withdrew due to subject decision. Missing data was not imputed.

Change in forced expiratory volume in 1 second as compared to normals for age (percent of predicted)

Outcome measures

Outcome measures
Measure
Study Drug
n=30 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=32 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Change in FEV1 (Percent of Predicted for Age)
1.055 percent of predicted vlaues
Interval -26.16 to 25.73
-5.62 percent of predicted vlaues
Interval -24.54 to 19.69

SECONDARY outcome

Timeframe: Baseline to end of study (24 weeks)

Population: Out of 70 subjects randomized, 65 completed the Week 12 visit and 62 completed the study. There were 6 withdrawals in the NAC group and 2 in Placebo. Five out of 6 subjects in the NAC group withdrew due to subject decision. Missing data was not imputed.

Forced expiratory volume in 1 second (Liters)

Outcome measures

Outcome measures
Measure
Study Drug
n=30 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=32 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
FEV1 (L)
2.5 Liter
Interval -21.92 to 30.28
-4.35 Liter
Interval -25.0 to 22.94

SECONDARY outcome

Timeframe: Baseline to end of study (24 weeks)

Population: Out of 70 subjects randomized, 65 completed the Week 12 visit and 62 completed the study. There were 6 withdrawals in the NAC group and 2 in Placebo. Five out of 6 subjects in the NAC group withdrew due to subject decision. Missing data was not imputed.

Difference in mid-expiratory flow rates between 25 to 75% of the vital capacity, in L/sec measured at the beginning of the study to the end of the study.

Outcome measures

Outcome measures
Measure
Study Drug
n=30 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=32 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
FEF 25-75% (L/Sec)
0.08 L/sec
Interval -0.39 to 0.88
-0.13 L/sec
Interval -1.22 to 0.39

SECONDARY outcome

Timeframe: Baseline to 24 weeks

Population: Out of 70 subjects randomized, 65 completed the Week 12 visit and 62 completed the study. There were 6 withdrawals in the NAC group and 2 in Placebo. Five out of 6 subjects in the NAC group withdrew due to subject decision. Missing data was not imputed.

Difference in the forced expiratory flow rate in mid-exhalation as a percent of predicted to standard values measured from baseline to the end of study (24 weeks).

Outcome measures

Outcome measures
Measure
Study Drug
n=30 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=32 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
FEF 25-75% (Percent of Predicted)
1.33 percent of predicted
Interval -16.46 to 25.32
-3.81 percent of predicted
Interval -25.2 to 9.78

OTHER_PRE_SPECIFIED outcome

Timeframe: Baseline to 24 weeks

Population: Sixteen subjects at Stanford University were enrolled as an initial safety cohort, and their data was used to evaluate the potential for NAC to cause PH in CF subjects.

Change in the diffusing capacity of carbon monoxide across the lung measured from baseline to end of 24-week study.

Outcome measures

Outcome measures
Measure
Study Drug
n=8 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=8 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Change in DLCO (ml/Min/mmHg) Over Time by Treatment Group
-0.32 ml/min/mm Hg
Standard Deviation 2.86 • Interval -4.76 to
-1.39 ml/min/mm Hg
Standard Deviation 3.93

OTHER_PRE_SPECIFIED outcome

Timeframe: Baseline to 24 weeks

Population: Sixteen subjects at Stanford University were enrolled as an initial safety cohort, and their data was used to evaluate the potential for NAC to cause PH in CF subjects. 1 subject in NAC cohort has missing data. It was not imputed

Change in measure of estimated right ventricular pressure over the 24-week study period

Outcome measures

Outcome measures
Measure
Study Drug
n=7 Participants
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=8 Participants
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Change in ECHO Tricuspid Regurgitation (mm Hg) Over Time by Treatment Group
31.5 mm Hg
Standard Deviation 6.02
31.75 mm Hg
Standard Deviation 5.84

Adverse Events

Study Drug

Serious events: 15 serious events
Other events: 33 other events
Deaths: 0 deaths

Placebo

Serious events: 17 serious events
Other events: 30 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Study Drug
n=36 participants at risk
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=34 participants at risk
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
Respiratory, thoracic and mediastinal disorders
Pulmonary Exacerbation
41.7%
15/36
50.0%
17/34

Other adverse events

Other adverse events
Measure
Study Drug
n=36 participants at risk
N-acetylcysteine (NAC) :PharmaNAC® 900 mg effervescent tablets in blister packs were supplied by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada).
Placebo
n=34 participants at risk
Identically packaged effervescent tablets that contained only the carrier were provided in blister packs by BioAdvantex Pharma, Inc. (Mississauga, ON, Canada)
General disorders
Fatigue
36.1%
13/36 • Number of events 17
23.5%
8/34 • Number of events 21
Investigations
Forced Expiratory Volume Decreased
25.0%
9/36 • Number of events 11
26.5%
9/34 • Number of events 14
Gastrointestinal disorders
Abdominal Pain Upper
5.6%
2/36 • Number of events 2
14.7%
5/34 • Number of events 6
Gastrointestinal disorders
Constipation
11.1%
4/36 • Number of events 5
2.9%
1/34 • Number of events 1
Gastrointestinal disorders
Diarrhoea
11.1%
4/36 • Number of events 5
8.8%
3/34 • Number of events 3
Gastrointestinal disorders
Nausea
13.9%
5/36 • Number of events 6
5.9%
2/34 • Number of events 3
Gastrointestinal disorders
Vomiting
8.3%
3/36 • Number of events 4
11.8%
4/34 • Number of events 4
General disorders
Chest Discomfort
8.3%
3/36 • Number of events 5
14.7%
5/34 • Number of events 7
General disorders
Non-cardiac Chest Pain
0.00%
0/36
8.8%
3/34 • Number of events 3
General disorders
Pain
8.3%
3/36 • Number of events 5
5.9%
2/34 • Number of events 3
General disorders
Pyrexia
11.1%
4/36 • Number of events 7
23.5%
8/34 • Number of events 14
Infections and infestations
sinusitis
8.3%
3/36 • Number of events 3
2.9%
1/34 • Number of events 1
Investigations
Pulmonary function test decreased
5.6%
2/36 • Number of events 3
8.8%
3/34 • Number of events 3
Investigations
Weight decreased
13.9%
5/36 • Number of events 6
14.7%
5/34 • Number of events 6
Investigations
white blood cell count increased
5.6%
2/36 • Number of events 2
2.9%
1/34 • Number of events 1
Musculoskeletal and connective tissue disorders
Back pain
5.6%
2/36 • Number of events 5
8.8%
3/34 • Number of events 3
Nervous system disorders
Sinus headache
11.1%
4/36 • Number of events 18
11.8%
4/34 • Number of events 6
Psychiatric disorders
Anxiety
5.6%
2/36 • Number of events 4
0.00%
0/34
Renal and urinary disorders
Nephrolitiasis
8.3%
3/36 • Number of events 3
0.00%
0/34
Respiratory, thoracic and mediastinal disorders
Cough
61.1%
22/36 • Number of events 55
61.8%
21/34 • Number of events 45
Respiratory, thoracic and mediastinal disorders
Dyspnea
13.9%
5/36 • Number of events 7
5.9%
2/34 • Number of events 2
Respiratory, thoracic and mediastinal disorders
Hemoptysis
22.2%
8/36 • Number of events 12
17.6%
6/34 • Number of events 8
Respiratory, thoracic and mediastinal disorders
Nasal congestion
5.6%
2/36 • Number of events 2
29.4%
10/34 • Number of events 11
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
13.9%
5/36 • Number of events 5
29.4%
10/34 • Number of events 16
Respiratory, thoracic and mediastinal disorders
Paranasal sinus hypersecretion
8.3%
3/36 • Number of events 5
2.9%
1/34 • Number of events 1
Respiratory, thoracic and mediastinal disorders
Postnasal drip
8.3%
3/36 • Number of events 4
2.9%
1/34 • Number of events 1
Respiratory, thoracic and mediastinal disorders
Productive cough
22.2%
8/36 • Number of events 10
38.2%
13/34 • Number of events 18
Respiratory, thoracic and mediastinal disorders
Rales
19.4%
7/36 • Number of events 10
23.5%
8/34 • Number of events 11
Respiratory, thoracic and mediastinal disorders
Respiratory tract congestion
19.4%
7/36 • Number of events 8
14.7%
5/34 • Number of events 9
Respiratory, thoracic and mediastinal disorders
Rhinorrhea
11.1%
4/36 • Number of events 6
17.6%
6/34 • Number of events 6
Respiratory, thoracic and mediastinal disorders
Sinus congestion
11.1%
4/36 • Number of events 5
11.8%
4/34 • Number of events 4
Respiratory, thoracic and mediastinal disorders
Wheezing
11.1%
4/36 • Number of events 6
17.6%
6/34 • Number of events 7
Skin and subcutaneous tissue disorders
Rash
2.8%
1/36 • Number of events 1
5.9%
2/34 • Number of events 2

Additional Information

Dr. Carol Conrad

Stanford University

Phone: 650-723-8325

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place