Trial Outcomes & Findings for A Study of the Bioequivalence of 70-mg Alendronate and 70-mg Alendronate in Combination With 5600 IU Vitamin D (MK0217A-253) (NCT NCT00803790)

NCT ID: NCT00803790

Last Updated: 2022-02-03

Results Overview

Bioequivalence was demonstrated by measuring the total urinary excretion of alendronate which was determined over a 36-hour period following single dose administration of 70-mg alendronate+5600 International Units (IU) vitamin D combination tablet and 70mg alendronate tablet alone. Urine for each treatment period was collected at -2 to 0 hours predose, 0 to 8, 8 to 24, and 24 to 36 hours postdose on Days 1 and 2.

Recruitment status

COMPLETED

Study phase

PHASE1

Target enrollment

318 participants

Primary outcome timeframe

Day 1-2 across the 36 hour urinary collection period (Periods 1 and 2)

Results posted on

2022-02-03

Participant Flow

This was a randomized, 2-part, crossover study. Each participant participated in one part of the study only (i.e., each participant participated only in Part I or only in Part II). A washout of at least 12 days separated each treatment period within each part of the study. 318 participants were enrolled (251 in Part 1 and 67 in Part 2).

Participant milestones

Participant milestones
Measure
Part 1 Alendronate+Vitamin D Combo, Then Alendronate
Participants in Part 1 received a single dose of 70mg alendronate+5600 International Units (IU) vitamin D combination tablet in Period 1 followed by a single dose of 70mg alendronate tablet in Period 2. A washout of at least 12 days separated each treatment period.
Part 1 Alendronate, Then Alendronate+Vitamin D Combo
Participants in Part 1 received a single dose of 70mg alendronate tablet in Period 1 followed by a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 2. A washout of at least 12 days separated each treatment period.
Part 2 Alendronate+Vitamin D Combo, Then Vitamin D
Participants in Part 2 received a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 1 followed by a single dose of 5600 IU vitamin D, administered as two 2800 IU tablets in Period 2. A washout of at least 12 days separated each treatment period.
Part 2 Vitamin D, Then Alendronate+Vitamin D Combo
Participants in Part 2 received a single dose of 5600 IU vitamin D, administered as two 2800 IU tablets in Period 1 followed by a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 2. A washout of at least 12 days separated each treatment period.
Period 1
STARTED
125
126
32
35
Period 1
COMPLETED
116
114
31
31
Period 1
NOT COMPLETED
9
12
1
4
Period 2
STARTED
116
114
31
31
Period 2
COMPLETED
114
114
30
30
Period 2
NOT COMPLETED
2
0
1
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Part 1 Alendronate+Vitamin D Combo, Then Alendronate
Participants in Part 1 received a single dose of 70mg alendronate+5600 International Units (IU) vitamin D combination tablet in Period 1 followed by a single dose of 70mg alendronate tablet in Period 2. A washout of at least 12 days separated each treatment period.
Part 1 Alendronate, Then Alendronate+Vitamin D Combo
Participants in Part 1 received a single dose of 70mg alendronate tablet in Period 1 followed by a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 2. A washout of at least 12 days separated each treatment period.
Part 2 Alendronate+Vitamin D Combo, Then Vitamin D
Participants in Part 2 received a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 1 followed by a single dose of 5600 IU vitamin D, administered as two 2800 IU tablets in Period 2. A washout of at least 12 days separated each treatment period.
Part 2 Vitamin D, Then Alendronate+Vitamin D Combo
Participants in Part 2 received a single dose of 5600 IU vitamin D, administered as two 2800 IU tablets in Period 1 followed by a single dose of 70mg alendronate+5600 IU vitamin D combination tablet in Period 2. A washout of at least 12 days separated each treatment period.
Period 1
Adverse Event
0
1
0
0
Period 1
Lost to Follow-up
1
2
0
0
Period 1
Withdrawal by Subject
8
6
0
0
Period 1
positive drug screen or personal reasons
0
3
0
4
Period 1
Protocol Violation
0
0
1
0
Period 2
Adverse Event
0
0
1
1
Period 2
Withdrawal by Subject
1
0
0
0
Period 2
Positive Drug Screen or Personal Reasons
1
0
0
0

Baseline Characteristics

A Study of the Bioequivalence of 70-mg Alendronate and 70-mg Alendronate in Combination With 5600 IU Vitamin D (MK0217A-253)

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Part 1
n=251 Participants
Alendronate+vitamin D combo, then alendronate: In Period 1 participants received a single dose of 70mg alendronate+5600 IU vitamin D combination tablet, and in Period 2 a single dose of 70mg alendronate tablet. A washout of at least 12 days separated each treatment period. Alendronate, then alendronate+vitamin D combo: In Period 1 participants received 70mg alendronate tablet, and in Period 2 a single dose of 70mg alendronate+5600 IU vitamin D combination tablet. A washout of at least 12 days separated each treatment period.
Part 2
n=67 Participants
Alendronate+vitamin D combo, then vitamin D: In Period 1 participants received a single dose of 70mg alendronate+5600 IU vitamin D combination tablet, and in Period 2 a single dose of 5600 IU vitamin D, administered as 2 x 2800 IU tablets. A washout of at least 12 days separated each treatment period. Vitamin D, then alendronate+vitamin D combo: In Period 1 participants received a single dose of 5600 IU vitamin D, administered as 2 x 2800 IU tablets, and in Period 2 a single dose of 70mg alendronate+5600 IU vitamin D combination tablet. A washout of at least 12 days separated each treatment period.
Total
n=318 Participants
Total of all reporting groups
Age, Customized
Between 18 and 78 years
251 participants
n=5 Participants
67 participants
n=7 Participants
318 participants
n=5 Participants
Sex: Female, Male
Female
132 Participants
n=5 Participants
29 Participants
n=7 Participants
161 Participants
n=5 Participants
Sex: Female, Male
Male
119 Participants
n=5 Participants
38 Participants
n=7 Participants
157 Participants
n=5 Participants

PRIMARY outcome

Timeframe: Day 1-2 across the 36 hour urinary collection period (Periods 1 and 2)

Population: 220 participants of the 251 enrolled in Part 1 were included in the statistical analysis. 31 participants were excluded: 23 were enrolled but did not complete both study periods and 8 participants had incomplete urine profiles in one or both periods.

Bioequivalence was demonstrated by measuring the total urinary excretion of alendronate which was determined over a 36-hour period following single dose administration of 70-mg alendronate+5600 International Units (IU) vitamin D combination tablet and 70mg alendronate tablet alone. Urine for each treatment period was collected at -2 to 0 hours predose, 0 to 8, 8 to 24, and 24 to 36 hours postdose on Days 1 and 2.

Outcome measures

Outcome measures
Measure
Alendronate+Vitamin D Combo
n=220 Participants
Single dose of 70mg alendronate+5600 IU vitamin D combination tablet
Alendronate
n=220 Participants
Single dose of 70mg alendronate tablet
Part 1: Urinary Excretion of Alendronate
133.6 μg
Standard Deviation 189.2
132.2 μg
Standard Deviation 273.4

PRIMARY outcome

Timeframe: Day 1 across the 80-hour plasma collection period (Period 1 and 2)

Population: 60 participants of the 67 enrolled in Part 2 were included in the statistical analysis. 7 participants that were enrolled, but did not complete both study periods were excluded.

The serum vitamin D pharmacokinetic parameter was calculated following the treatment of 70mg alendronate+5600 IU vitamin D combination tablet and 5600 IU vitamin D tablet on Day 1: area under the plasma concentration-time curve (AUC0-80hr). Serum samples for determination of vitamin D concentrations were obtained at -2 and 0 hrs predose on Day 1 and at 2, 3, 5, 7, 9, 12, 16, 24, 36, 48, 72 and 80 hours post dose for each treatment period.

Outcome measures

Outcome measures
Measure
Alendronate+Vitamin D Combo
n=60 Participants
Single dose of 70mg alendronate+5600 IU vitamin D combination tablet
Alendronate
n=60 Participants
Single dose of 70mg alendronate tablet
Part II: AUC (Area Under the Plasma Concentration-time Curve) of Vitamin D
490.2 ng*hr/mL
Standard Deviation 259.6
518.7 ng*hr/mL
Standard Deviation 269.8

SECONDARY outcome

Timeframe: Day 1 across the 80-hour plasma collection period (Periods 1 and 2)

Population: 60 participants of the 67 enrolled in Part 2 were included in the statistical analysis. 7 participants that were enrolled, but did not complete both study periods were excluded.

Serum vitamin D pharmacokinetic parameter was calculated for the following: maximum concentration of drug observed in serum (Cmax). Serum samples for determination of vitamin D concentrations were obtained at -2 and 0 hrs predose on Day 1 and at 2, 3, 5, 7, 9, 12, 16, 24, 36, 48, 72 and 80 hours post dose for each treatment period.

Outcome measures

Outcome measures
Measure
Alendronate+Vitamin D Combo
n=60 Participants
Single dose of 70mg alendronate+5600 IU vitamin D combination tablet
Alendronate
n=60 Participants
Single dose of 70mg alendronate tablet
Part II : Maximum Concentration (Cmax) of Vitamin D
12.2 ng/ml
Standard Deviation 5.6
13.0 ng/ml
Standard Deviation 5.9

Adverse Events

Alendronate+Vitamin D Combo

Serious events: 0 serious events
Other events: 21 other events
Deaths: 0 deaths

Alendronate

Serious events: 0 serious events
Other events: 12 other events
Deaths: 0 deaths

Vitamin D

Serious events: 0 serious events
Other events: 9 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
Alendronate+Vitamin D Combo
n=302 participants at risk
Single dose of 70 mg alendronate+5600 IU vitamin D combination tablet
Alendronate
n=241 participants at risk
Single dose of 70mg alendronate tablet
Vitamin D
n=65 participants at risk
Single dose of 5600 IU vitamin D, administered as 2 x 2800 IU tablets
Nervous system disorders
Headache
7.0%
21/302 • 8 weeks
There were 28 missing participants whose discontinuation did not allow for them to receive the treatments assigned in both Periods 1 and 2 (Alendronate= 10, Alendronate+Vitamin D Combo= 16, Vitamin D= 2).
5.0%
12/241 • 8 weeks
There were 28 missing participants whose discontinuation did not allow for them to receive the treatments assigned in both Periods 1 and 2 (Alendronate= 10, Alendronate+Vitamin D Combo= 16, Vitamin D= 2).
13.8%
9/65 • 8 weeks
There were 28 missing participants whose discontinuation did not allow for them to receive the treatments assigned in both Periods 1 and 2 (Alendronate= 10, Alendronate+Vitamin D Combo= 16, Vitamin D= 2).

Additional Information

Vice President, Late Stage Development Group Leader

Merck Sharp & Dohme Corp

Results disclosure agreements

  • Principal investigator is a sponsor employee The SPONSOR must have the opportunity to review all proposed abstracts, manuscripts, or presentations regarding this study 60 days prior to submission for publication/presentation. Any information identified by the SPONSOR as confidential must be deleted prior to submission. SPONSOR review can be expedited to meet publication timelines.
  • Publication restrictions are in place

Restriction type: OTHER