Assessment of Three Formulations of the Candidate Vaccine AMA 1 in Healthy Dutch Adult Volunteers

NCT ID: NCT00730782

Last Updated: 2008-08-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

56 participants

Study Classification

INTERVENTIONAL

Study Start Date

2005-11-30

Study Completion Date

2008-08-31

Brief Summary

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The objective of this study was to evaluate the safety of a candidate malaria vaccine (PfAMA-1) at 3 doses given at monthly intervals of 2 different dosages of AMA-1 (10 μg or 50 μg ) adjuvanted either with alum hydroxide or AS02A or Montanide ISA 720 in healthy adults not previously exposed to the parasite Plasmodium falciparum.

Detailed Description

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Conditions

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Plasmodium Falciparum Malaria

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Participants

Study Groups

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1

The experimental vaccine PfAMA1 formulated in Alhydrogel

Group Type ACTIVE_COMPARATOR

PfAMA-1-FVO[25-545]

Intervention Type BIOLOGICAL

Subcutaneous vaccination of 0.5ml of two dosage 10 and 50ug PfAMA1

2

The experimental vaccine PfAMA1 formulated in Montanide ISA720

Group Type ACTIVE_COMPARATOR

PfAMA-1-FVO[25-545]

Intervention Type BIOLOGICAL

Subcutaneous vaccination of 0.5ml of two dosage 10 and 50ug PfAMA1

3

The experimental vaccine PfAMA1 formulated in ASO2A

Group Type ACTIVE_COMPARATOR

PfAMA-1-FVO[25-545]

Intervention Type BIOLOGICAL

Subcutaneous vaccination of 0.5ml of two dosage 10 and 50ug PfAMA1

Interventions

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PfAMA-1-FVO[25-545]

Subcutaneous vaccination of 0.5ml of two dosage 10 and 50ug PfAMA1

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Age \> 18 and \< 45 years healthy volunteers.
* General good health based on history and clinical examination.
* All volunteers have to sign the informed consent form.
* Negative pregnancy test.
* Use of adequate contraception for females up to three months after the third injection (D140).
* Reachable by phone during the whole study period (18 months).

Exclusion Criteria

* History of malaria or residence in malaria endemic areas within the past six months.
* Positive serology for malaria antigen PfAMA-1
* Previously participated in any malaria vaccine study
* Symptoms, physical signs and laboratory values suggestive of systemic disorders, including renal, hepatic, cardiovascular, pulmonary, skin, immunodeficiency, psychiatric and other conditions, which could interfere with the interpretation of the study results or compromise the health of the volunteers.
* Any laboratory abnormalities on screened blood samples beyond the normal range, as defined at UMC St Radboud. Positive HIV, HBV or HCV tests.
* Volunteers should not be enrolled in any other clinical trial during the whole trial period.
* Volunteers should not receive chronic medication, especially immunosuppressive agents (steroids, immunomodulating or immunosuppressive drugs) during the three months preceding the screening visit or during the study period.
* Pregnant or lactating women.
* Volunteers unable to give written informed consent.
* Volunteers unable to be closely followed for social, geographic or psychological reasons.
* Previous history of drug or alcohol abuse interfering with normal social function during a period of one year prior to enrollment in the study.
* Volunteers should not perform exercise four hours before blood draw and should not donate blood for non study-related purposes during the entire duration of the study.
* Known hypersensitivity to any of the vaccine components (adjuvant or peptide).
* Volunteers are not allowed to receive any vaccination or gammaglobulin during a period three months prior to the first immunization and up to six months after the 3rd immunization. If a vaccination is necessary during this period, the volunteer will be withdrawn from the study.
* Volunteers are not allowed to travel to malaria endemic countries during the study period.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Radboud University Medical Center

OTHER

Sponsor Role collaborator

Biomedical Primate Research Centre

OTHER

Sponsor Role collaborator

GlaxoSmithKline

INDUSTRY

Sponsor Role collaborator

Seppic

INDUSTRY

Sponsor Role collaborator

European Vaccine Initiative

OTHER

Sponsor Role lead

Responsible Party

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Radboud University Nijmegen Medical Centre

Principal Investigators

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Robert Sauerwein, Prof. MD

Role: PRINCIPAL_INVESTIGATOR

Radboud University Medical Center

Locations

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Radboud University Nijmegen Medical Centre

Nijmegen, , Netherlands

Site Status

Biomedical Primate Research Centre

Rijswijk, , Netherlands

Site Status

Countries

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Netherlands

References

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Remarque EJ, Roestenberg M, Younis S, Walraven V, van der Werff N, Faber BW, Leroy O, Sauerwein R, Kocken CH, Thomas AW. Humoral immune responses to a single allele PfAMA1 vaccine in healthy malaria-naive adults. PLoS One. 2012;7(6):e38898. doi: 10.1371/journal.pone.0038898. Epub 2012 Jun 29.

Reference Type DERIVED
PMID: 22768052 (View on PubMed)

Roestenberg M, Remarque E, de Jonge E, Hermsen R, Blythman H, Leroy O, Imoukhuede E, Jepsen S, Ofori-Anyinam O, Faber B, Kocken CH, Arnold M, Walraven V, Teelen K, Roeffen W, de Mast Q, Ballou WR, Cohen J, Dubois MC, Ascarateil S, van der Ven A, Thomas A, Sauerwein R. Safety and immunogenicity of a recombinant Plasmodium falciparum AMA1 malaria vaccine adjuvanted with Alhydrogel, Montanide ISA 720 or AS02. PLoS One. 2008;3(12):e3960. doi: 10.1371/journal.pone.0003960. Epub 2008 Dec 18.

Reference Type DERIVED
PMID: 19093004 (View on PubMed)

Other Identifiers

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EUDRACT No 2005-00232-24

Identifier Type: -

Identifier Source: secondary_id

AMA-1_1_03

Identifier Type: -

Identifier Source: org_study_id