Repeated DermaVir Immunizations in HIV-1 Infected Treatment-naïve Patients

NCT ID: NCT00711230

Last Updated: 2020-01-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

36 participants

Study Classification

INTERVENTIONAL

Study Start Date

2008-04-30

Study Completion Date

2015-01-01

Brief Summary

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DermaVir is a synthetic pathogen-like nanomedicine. The active pharmaceutical ingredient is a single plasmid DNA expressing fifteen HIV antigens that assemble to HIV-like particles. These particles are safe; replication, integration and reverse transcription deficient. DermaVir is targeted to Langerhans cells by topical administration with DermaPrep. Langerhans cells with DermaVir migrate to lymph nodes and induce HIV-specific T cells that can kill HIV-infected cells.

GIEU006 is a Phase II randomized, placebo-controlled, dose-finding, double-blinded, multicenter study to assess the safety, tolerability, immunogenicity, and preliminary antiretroviral activity of DermaVir in antiretroviral therapy naïve adults with HIV-infection.

Detailed Description

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Patients were randomized into one of the following 6 arms:

* Arm 1: Low dose DermaVir (0.2 mg DNA in 2 DermaPrep patches, n=9)
* Arm 2: Low dose Placebo (2 DermaPrep patches, n=3)
* Arm 3: Medium dose DermaVir (0.4 mg DNA in 4 DermaPrep patches, n=9)
* Arm 4: Medium dose Placebo (4 DermaPrep patches, n=3)
* Arm 5: High dose DermaVir (0.8 mg DNA in 8 DermaPrep patches, n=9)
* Arm 6: High dose Placebo (8 DermaPrep patches, n=3) DermaPrep Patch size: 80 cm2. DermaVir Standard Unit per patch is 0.1 mg DNA = 0.8 mL of DermaVir nanomedicine.

The patch sites for immunization are preferably the left or right upper back and left or right upper ventral thigh. The same skin sites should be used for all immunizations.

Immunization schedule (Days): 0, 42, 84, and 126.

The total DermaVir dose:

* Low dose: 0.8 mg DNA
* Medium dose: 1.6 mg DNA
* High Dose: 3.2 mg DNA

DermaVir immunizations were administered over an 18-week period Primary endpoint: 24 weeks Safety follow up: 234 weeks

Conditions

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HIV Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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1: Low dose DermaVir

* Dosage: 0.2 mg DNA
* Dosage form: 1.6 mL DNA/PEIm nanomedicine
* Administration with 2 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 DermaVir treatments)

Group Type EXPERIMENTAL

DermaVir

Intervention Type BIOLOGICAL

2: Low dose Placebo

* Dosage form: 1.6 mL Placebo
* Administration with 2 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 Placebo treatments)

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

glucose/dextrose

3: Medium dose DermaVir

* Dosage: 0.4 mg DNA
* Dosage form: 3.2 mL DNA/PEIm nanomedicine
* Administration with 4 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 DermaVir treatments)

Group Type EXPERIMENTAL

DermaVir

Intervention Type BIOLOGICAL

4: Medium dose Placebo

* Dosage form: 1.6 mL Placebo
* Administration with 4 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 Placebo treatments)

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

glucose/dextrose

5: High dose DermaVir

* Dosage: 0.8 mg DNA
* Dosage form: 6.4 mL DNA/PEIm nanomedicine
* Administration with 8 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 DermaVir treatments)

Group Type EXPERIMENTAL

DermaVir

Intervention Type BIOLOGICAL

6: High dose Placebo

* Dosage form: 6.4 mL Placebo
* Administration with 8 DermaPrep patches
* Frequency: every six weeks
* Duration: 18 weeks (4 Placebo treatments)

Group Type EXPERIMENTAL

Placebo

Intervention Type BIOLOGICAL

glucose/dextrose

Interventions

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DermaVir

Intervention Type BIOLOGICAL

Placebo

glucose/dextrose

Intervention Type BIOLOGICAL

Other Intervention Names

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LC002

Eligibility Criteria

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Inclusion Criteria

* HIV antibody positive
* Plasma HIV RNA value ≥5,000 copies/mL and ≤ 150,000 c/mL
* Antiretroviral therapy naïve
* Documented CD4+ T-cell count at screening ≥400 cells/mm3

Exclusion Criteria

* No skin disease
* No tattoos, or changes in pigmentation at the selected skin immunization sites
* No acute or chronic illness (e.g Hepatitis C)
* No chronic autoimmune diseases
* No treatment with any immune modulating agents
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Universitätsklinikum Hamburg-Eppendorf

OTHER

Sponsor Role collaborator

Genetic Immunity

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jan Van Lunzen, PhD, MD

Role: PRINCIPAL_INVESTIGATOR

Universitätsklinikum Hamburg-Eppendorf

Locations

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ifi-Medizin GmbH at the Asklepios Klinik St. Georg

Hamburg, , Germany

Site Status

ICH Grindel

Hamburg, , Germany

Site Status

University Medical Center Hamburg-Eppendorf

Hamburg, , Germany

Site Status

Countries

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Germany

References

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Lisziewicz J, Bakare N, Calarota SA, Banhegyi D, Szlavik J, Ujhelyi E, Toke ER, Molnar L, Lisziewicz Z, Autran B, Lori F. Single DermaVir immunization: dose-dependent expansion of precursor/memory T cells against all HIV antigens in HIV-1 infected individuals. PLoS One. 2012;7(5):e35416. doi: 10.1371/journal.pone.0035416. Epub 2012 May 9.

Reference Type BACKGROUND
PMID: 22590502 (View on PubMed)

Lisziewicz J, Toke ER. Nanomedicine applications towards the cure of HIV. Nanomedicine. 2013 Jan;9(1):28-38. doi: 10.1016/j.nano.2012.05.012. Epub 2012 May 30.

Reference Type BACKGROUND
PMID: 22659241 (View on PubMed)

Lorincz O, Toke ER, Somogyi E, Horkay F, Chandran PL, Douglas JF, Szebeni J, Lisziewicz J. Structure and biological activity of pathogen-like synthetic nanomedicines. Nanomedicine. 2012 May;8(4):497-506. doi: 10.1016/j.nano.2011.07.013. Epub 2011 Aug 10.

Reference Type BACKGROUND
PMID: 21839051 (View on PubMed)

Toke ER, Lorincz O, Somogyi E, Lisziewicz J. Rational development of a stable liquid formulation for nanomedicine products. Int J Pharm. 2010 Jun 15;392(1-2):261-7. doi: 10.1016/j.ijpharm.2010.03.048. Epub 2010 Mar 25.

Reference Type BACKGROUND
PMID: 20347027 (View on PubMed)

Lori F. DermaVir: a plasmid DNA-based nanomedicine therapeutic vaccine for the treatment of HIV/AIDS. Expert Rev Vaccines. 2011 Oct;10(10):1371-84. doi: 10.1586/erv.11.118.

Reference Type BACKGROUND
PMID: 21988301 (View on PubMed)

Somogyi E, Xu J, Gudics A, Toth J, Kovacs AL, Lori F, Lisziewicz J. A plasmid DNA immunogen expressing fifteen protein antigens and complex virus-like particles (VLP+) mimicking naturally occurring HIV. Vaccine. 2011 Jan 17;29(4):744-53. doi: 10.1016/j.vaccine.2010.11.019. Epub 2010 Nov 23.

Reference Type BACKGROUND
PMID: 21109034 (View on PubMed)

Calarota SA, Weiner DB, Lori F, Lisziewicz J. Induction of HIV-specific memory T-cell responses by topical DermaVir vaccine. Vaccine. 2007 Apr 20;25(16):3070-4. doi: 10.1016/j.vaccine.2007.01.024. Epub 2007 Jan 22.

Reference Type BACKGROUND
PMID: 17292518 (View on PubMed)

Lisziewicz J, Trocio J, Whitman L, Varga G, Xu J, Bakare N, Erbacher P, Fox C, Woodward R, Markham P, Arya S, Behr JP, Lori F. DermaVir: a novel topical vaccine for HIV/AIDS. J Invest Dermatol. 2005 Jan;124(1):160-9. doi: 10.1111/j.0022-202X.2004.23535.x.

Reference Type BACKGROUND
PMID: 15654970 (View on PubMed)

Lori F, Trocio J, Bakare N, Kelly LM, Lisziewicz J. DermaVir, a novel HIV immunisation technology. Vaccine. 2005 Mar 18;23(17-18):2030-4. doi: 10.1016/j.vaccine.2005.01.004.

Reference Type BACKGROUND
PMID: 15755566 (View on PubMed)

Lisziewicz J, Trocio J, Xu J, Whitman L, Ryder A, Bakare N, Lewis MG, Wagner W, Pistorio A, Arya S, Lori F. Control of viral rebound through therapeutic immunization with DermaVir. AIDS. 2005 Jan 3;19(1):35-43. doi: 10.1097/00002030-200501030-00004.

Reference Type BACKGROUND
PMID: 15627031 (View on PubMed)

Related Links

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http://www.geneticimmunity.com

Genetic Immunity's homepage

http://hivandhepatitis.com/2010_conference/AIDS2010/docs/post/lunzen.pdf

DermaVir for initial treatment of HIV-infected subjects demonstrates preliminary safety, immunogenicity and HIV-RNA reduction versus placebo immunization

Other Identifiers

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2007-001955-20

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

DermaVir Phase II

Identifier Type: -

Identifier Source: org_study_id

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