Trial Outcomes & Findings for Statin Therapy for Ischemic and Nonischemic Cardiomyopathy (NCT NCT00701220)

NCT ID: NCT00701220

Last Updated: 2023-07-18

Results Overview

Change in Endothelial Progenitor Cells (EPC) was assessed by extracting white blood cells from blood samples and labeling the white blood cells using the ALDEFLOUR system. Facscan analysis was then performed first isolating lymphocytic and monocytic cell lines, which contain the EPC populations, using forward versus side scatter plots. These cells were then scanned for fluorescence in the ALDEFLUOR frequency range allowing enumeration of progenitor cell lines. The measures of fluorescent cells was compared to numbers of unstained cells having the same back scatter range to correct for any background fluorescence.

Recruitment status

COMPLETED

Study phase

PHASE4

Target enrollment

9 participants

Primary outcome timeframe

Baseline - 6 months

Results posted on

2023-07-18

Participant Flow

Patients were eligible for enrollment if they had dilated cardiomyopathy w/ an established ejection fraction \< 35% on a nonischemic or ischemic basis. All pts had clinical indication for treatment w/ a statin Rx \& did not receive a statin medication prior to enrollment in the study.

Patients with cardiomyopathy on the basis of valvular heart disease or who had a myocardial infarction within six months prior to study enrollment were excluded from the study.

Participant milestones

Participant milestones
Measure
Ischemic Cardiomyopathy
Patients with Ischemic Cardiomyopathy receiving Lipitor.
NonIschemic Cardiomyopathy
NonIschemic Cardiomyopathy receiving Lipitor treatment
Overall Study
STARTED
3
6
Overall Study
COMPLETED
1
4
Overall Study
NOT COMPLETED
2
2

Reasons for withdrawal

Reasons for withdrawal
Measure
Ischemic Cardiomyopathy
Patients with Ischemic Cardiomyopathy receiving Lipitor.
NonIschemic Cardiomyopathy
NonIschemic Cardiomyopathy receiving Lipitor treatment
Overall Study
Withdrawal by Subject
0
2
Overall Study
Protocol Violation
2
0

Baseline Characteristics

Statin Therapy for Ischemic and Nonischemic Cardiomyopathy

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Ischemic Cardiomyopathy
n=1 Participants
Patients with Ischemic Cardiomyopathy receiving Lipitor.
NonIschemic Cardiomyopathy
n=4 Participants
NonIschemic Cardiomyopathy receiving Lipitor treatment
Total
n=5 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=93 Participants
0 Participants
n=4 Participants
0 Participants
n=27 Participants
Age, Categorical
Between 18 and 65 years
1 Participants
n=93 Participants
4 Participants
n=4 Participants
5 Participants
n=27 Participants
Age, Categorical
>=65 years
0 Participants
n=93 Participants
0 Participants
n=4 Participants
0 Participants
n=27 Participants
Age, Continuous
51.33 years
STANDARD_DEVIATION 15.29 • n=93 Participants
53.66 years
STANDARD_DEVIATION 9.71 • n=4 Participants
52.11 years
STANDARD_DEVIATION 17.37 • n=27 Participants
Sex: Female, Male
Female
0 Participants
n=93 Participants
1 Participants
n=4 Participants
1 Participants
n=27 Participants
Sex: Female, Male
Male
1 Participants
n=93 Participants
3 Participants
n=4 Participants
4 Participants
n=27 Participants
Region of Enrollment
United States
1 participants
n=93 Participants
4 participants
n=4 Participants
5 participants
n=27 Participants

PRIMARY outcome

Timeframe: Baseline - 6 months

Change in Endothelial Progenitor Cells (EPC) was assessed by extracting white blood cells from blood samples and labeling the white blood cells using the ALDEFLOUR system. Facscan analysis was then performed first isolating lymphocytic and monocytic cell lines, which contain the EPC populations, using forward versus side scatter plots. These cells were then scanned for fluorescence in the ALDEFLUOR frequency range allowing enumeration of progenitor cell lines. The measures of fluorescent cells was compared to numbers of unstained cells having the same back scatter range to correct for any background fluorescence.

Outcome measures

Outcome measures
Measure
Ischemic Cardiomyopathy
n=1 Participants
Patients with Ischemic Cardiomyopathy receiving Lipitor (atorvastatin calcium).
NonIschemic Cardiomyopathy
n=4 Participants
NonIschemic Cardiomyopathy receiving Lipitor (Atorvastatin Calcium) treatment
Change in Endothelial Progenitor Cells
-10.01 Percentage of EPCs
Standard Deviation 0
5.19 Percentage of EPCs
Standard Deviation 0.517

PRIMARY outcome

Timeframe: Baseline - 6 months

The primary outcome measure is the percentage of cells of total analyzed that are positive for the Aldofluor assay. This commercially available assay tests cells for the enzyme aldehyde dehydrogenase that is expressed in stem cells and other early life cycle undifferentiated cells. It thus provides a measure of circulating primordial cells or cells that may serve as progenitors of destination mature cells. The percentages are designed from Facscan analysis of prepared blood cells and are quantified by quadrants defined by specific fluorescent bands and cell size distribution. The quantification of cells thus defined in the four quadrants is automatically determined by software resident in the facscan analysis system.

Outcome measures

Outcome measures
Measure
Ischemic Cardiomyopathy
n=1 Participants
Patients with Ischemic Cardiomyopathy receiving Lipitor (atorvastatin calcium).
NonIschemic Cardiomyopathy
n=4 Participants
NonIschemic Cardiomyopathy receiving Lipitor (Atorvastatin Calcium) treatment
Percentage Aldofluor Positive Cells
6.37 Percentage Aldefluor positive cells
Standard Deviation 0
5.7 Percentage Aldefluor positive cells
Standard Deviation 0.5

Adverse Events

Ischemic Cardiomyopathy

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

NonIschemic Cardiomyopathy

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Philip F. Binkley, MD, MPH

The Ohio State University

Phone: (614) 293-3956

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place