Vaccination of Patients With Breast Cancer With Dendritic Cell/Tumor Fusions and IL-12

NCT ID: NCT00622401

Last Updated: 2017-11-14

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

8 participants

Study Classification

INTERVENTIONAL

Study Start Date

2009-12-31

Study Completion Date

2014-10-31

Brief Summary

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The purpose of this study is to test the safety of an investigational Dendritic Cell/Tumor Fusion vaccine given with IL-12 for patients with breast cancer.

RATIONALE: Vaccines made from a person's tumor cells and white blood cells may help the body build an effective immune response to kill tumor cells. Interleukin-12 may stimulate the white blood cells to kill tumor cells. Giving vaccine therapy together with interleukin-12 may kill more tumor cells.

PURPOSE: This phase I/II trial is studying the side effects and best dose of interleukin-12 when given together with vaccine therapy and to see how well they work in treating women with stage IV breast cancer.

Detailed Description

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TUMOR COLLECTION: Tumor cells will be collected from the participant to make the study vaccine. Based on the location of the tumor, a decision will be made as to the best approach to obtain these cells.

DENDRITIC CELL COLLECTION: Participants will undergo a procedure known as leukapheresis to obtain their dendritic cells (this procedure may be done before or after the tumor cells have been obtained). This procedure takes about 2-4 hours. If not enough cells are collected, the participant may be asked to return for an additional leukapheresis procedure. If sufficient number of cells are obtained, tumor cells and dendritic cells will then be fused (combined together to make one larger cell) together in the laboratory and divided into the appropriate dose for administration.

TREATMENT: Treatment will consist of an injection of tumor cells fused with dendritic cells under the skin every 3 weeks for a total of 9 weeks. The dose that the participant receives will depend on the total number of fusion cells that are made.

STUDY COHORTS: The first group of three participants will receive the DC/Tumor Fusion study vaccine alone. The next group of 3 participants will receive the DC/Tumor Fusion study vaccine with a low dose of Il-12. If there are no significant side effects the following groups of subjects will be treated with the DC/Tumor Fusion study vaccine and a higher dose of Il-12.

PATIENT MONITORING: Participants will be carefully monitored during the study period and the following tests and procedures will be performed: physical exams (weekly); blood collections (weekly); DC/Tumor Fusion study vaccine Journal (for the participant to record any side effects or other medications they may be taking); tumor cells skin test (before the first vaccine and one month following the last vaccine); skin biopsy at the site of the vaccination administration, accessible tumor site, or if there is a local reaction site.

Conditions

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Breast Cancer

Keywords

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stage IV breast cancer dendritic cell vaccine tumor fusion vaccine IL-12

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Group 1

Dendritic Cell/Tumor Fusion Vaccine Only

Group Type EXPERIMENTAL

Dendritic Cell/Tumor Fusion Vaccine

Intervention Type BIOLOGICAL

Vaccine is derived from the participants dendritic cells and tumor cells

Group 2

Dendritic Cell/tumor fusion vaccine and low dose IL-12

Group Type EXPERIMENTAL

Dendritic Cell/Tumor Fusion Vaccine

Intervention Type BIOLOGICAL

Vaccine is derived from the participants dendritic cells and tumor cells

Interleukin-12

Intervention Type DRUG

Given subcutaneously at dose of 30ng/kg

Group 3

Dendritic Cell/tumor fusion vaccine and higher dose IL-12

Group Type EXPERIMENTAL

Dendritic Cell/Tumor Fusion Vaccine

Intervention Type BIOLOGICAL

Vaccine is derived from the participants dendritic cells and tumor cells

Interleukin-12

Intervention Type DRUG

Given subcutaneously at dose of 100ng/kg

Interventions

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Dendritic Cell/Tumor Fusion Vaccine

Vaccine is derived from the participants dendritic cells and tumor cells

Intervention Type BIOLOGICAL

Interleukin-12

Given subcutaneously at dose of 30ng/kg

Intervention Type DRUG

Interleukin-12

Given subcutaneously at dose of 100ng/kg

Intervention Type DRUG

Other Intervention Names

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DC/tumor cell fusion vaccine IL-12 rhIL-12 IL-12 rhIL-12

Eligibility Criteria

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Inclusion Criteria

* Stage IV breast cancer with measurable disease and accessible tumor
* ECOG Performance Status 0-2 with greater than six week life expectancy
* 18 years of age or older
* Laboratory values as outlined in the protocol

Exclusion Criteria

* Patients must not have received other immunotherapy treatment in the three months prior to the initial vaccination
* Patients may not be on herceptin therapy during this protocol and may not have received it for four weeks prior to initial vaccination
* Patients must not have received weekly chemotherapy or hormonal treatment for two weeks prior to the initial vaccination and must not have received monthly chemotherapy for four weeks prior to the initial vaccination
* Clinical evidence of CNS disease
* Clinically significant autoimmune disease
* Patients who are HIV+
* Serious intercurrent illness such as infection requiring IV antibiotics, or significant cardiac disease characterized by significant arrhythmia, ischemic coronary disease or congestive heart failure
* Pregnant of lactating women will be excluded, all premenopausal women must undergo pregnancy testing
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Dana-Farber Cancer Institute

OTHER

Sponsor Role collaborator

Brigham and Women's Hospital

OTHER

Sponsor Role collaborator

Harvard Medical School (HMS and HSDM)

OTHER

Sponsor Role collaborator

United States Department of Defense

FED

Sponsor Role collaborator

National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Beth Israel Deaconess Medical Center

OTHER

Sponsor Role lead

Responsible Party

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David Avigan, MD

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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David Avigan, MD

Role: PRINCIPAL_INVESTIGATOR

Beth Israel Deaconess Medical Center

Locations

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Beth Israel Deaconess Medical Center

Boston, Massachusetts, United States

Site Status

Countries

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United States

Other Identifiers

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NCI 6040

Identifier Type: -

Identifier Source: secondary_id

U01CA062490

Identifier Type: NIH

Identifier Source: secondary_id

View Link

P30CA006516

Identifier Type: NIH

Identifier Source: secondary_id

View Link

03-221

Identifier Type: -

Identifier Source: org_study_id

NCT00731406

Identifier Type: -

Identifier Source: nct_alias