Exercise Changes to Peripheral Blood Mononuclear Cells in Children

NCT ID: NCT00585403

Last Updated: 2021-01-25

Basic Information

• Recruitment Status: WITHDRAWN
• Study Classification: OBSERVATIONAL
• Study Start Date: 2004-12-31
• Study Completion Date: 2004-12-31

Brief Summary

The goal of this research is to determine how the peripheral immune system is altered by exercise and differences related to gender, pubertal status and health.

Detailed Description

SPECIFIC AIMS:

1. To systematically measure for the first time in healthy children and adolescents the effects of brief bouts of exercise on:

1. Numbers of circulating PMBCs, their subsets and key intercellular adhesion molecules (ICAMs).

2. PBMC gene regulation of stress, inflammatory, and growth/repair mediators [including: interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma), growth hormone (GH), insulin-like growth factor-I (IGF-I), heat shock proteins (Hsp)].

3. Circulating (serum) and intracellular PBMC levels of key mediators by flow cytometry and cell culture techniques.

4. Circulating endogenous triggers of PBMC mediator responses-soluble Hsp, IL-6, and F2-isoprostanes.

2. To determine how the acute PBMC responses are altered by gender, pubertal status, body composition (measured by whole-body and regional DEXA), and fitness (measured by progressive cycle ergometry and gas exchange).

3. To determine the relationship in healthy children and adolescents among acute PBMC responses to exercise, biochemical precursors of the metabolic syndrome (insulin, glucose, lipids), and the balance of the TH1/TH2 immune response.

Conditions

Physical Fitness; Obesity; Child Development; Growth

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

Children

Early and late pubertal girls and boys

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• appropriate Tanner Stage
• No evidence of disease or disability

Exclusion Criteria

• no use of antiinflammatory medications, alcohol, illegal drugs or bronchodilators
• elite children participating in extensive exercise or dance programs
• pregnant

• Minimum Eligible Age: 8 Years
• Maximum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of California, Irvine

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Dan M Cooper, M.D.

Role: PRINCIPAL_INVESTIGATOR

Univ. California, Irvine, CA

Locations

General Clinical Research Center

Orange, California, United States

Countries

United States

Other Identifiers

HL-80947

Identifier Type: -

Identifier Source: secondary_id

PBMC Exercise [2004-3967]

Identifier Type: -

Identifier Source: org_study_id