N-Acetylcysteine and Arginine Administration in Diabetic Patients

NCT ID: NCT00569465

Last Updated: 2007-12-07

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE4

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2005-01-31

Study Completion Date

2006-01-31

Brief Summary

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It has been demonstrated that the nitric oxide production is reduced in type 2 diabetic patients and that cardiovascular complications represent 80% of the causes of death in these patients. As nitric oxide is able to reduce platelet aggregation, increase the relaxation of smooth muscle cells, and reduce plasminogen activator inhibitor-1 and endothelin, we hypothesized that nitric oxide deficiency is responsable for the cardiovascular disease in type 2 diabetes mellitus. Arginine and N-acetylcysteine, precursor and enhancer of the nitric oxide synthesis respectively, are able to increase nitric oxide production.

Aim of the study is to evaluate the effect of arginine and N-acetylcysteine administration on arterial blood pressure and different metabolic parameters in patients with type 2 diabetes mellitus and hypertension.

Subjects and methods. 24 male subjects, randomly divided in two groups, will be studied. These subjects will undergo a treatment with arginine (1200 mg once a day) plus N-acetylcysteine (600 mg twice a day) or placebo for six months. Basal and final evaluations include:

* general examination
* ABPM (ambulatory blood pressure monitoring)
* HbA1c, total-cholesterol, HDL-cholesterol, LDL-cholesterol, oxidized LDLs, triglycerides, reduced/oxidized glutathione ratio in red blood cells, nitrites/nitrates, asymmetrical and symmetrical dimethyl-arginine, nitrotyrosine, arginine, homocysteine, C-reactive protein, interleukin-6, tumor necrosis factor-α, intercellular and vascular-cell adhesion molecules, plasminogen activator inhibitor-1 and fibrinogen
* the ultrasound assessment of the intima-media thickness after endothelium-dependent flow-mediated vasodilation of the brachial artery

Expected results. Increase of nitric oxide production and reduction of arterial blood pressure and oxidative parameters.

Detailed Description

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Conditions

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Type 2 Diabetes Mellitus Hypertension

Keywords

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nitric oxide oxidative stress diabetes hypertension endothelial dysfunction

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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A

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DRUG

3 vials a day

B

Group Type EXPERIMENTAL

Arginine

Intervention Type DRUG

1200 mg, once a day

Acetylcysteine

Intervention Type DRUG

600 mg twice a day

Interventions

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Arginine

1200 mg, once a day

Intervention Type DRUG

Acetylcysteine

600 mg twice a day

Intervention Type DRUG

Placebo

3 vials a day

Intervention Type DRUG

Other Intervention Names

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Zentrum Acetilcisteina

Eligibility Criteria

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Inclusion Criteria

* Male subjects
* Age between 40 and 70 years
* Type 2 diabetes mellitus and hypertension
* Mean 24h arterial blood pressure after the wash-out period: systolic \>136mmHg and/or diastolic \>86 mmHh
* Written informed consent

Exclusion Criteria

* Female subjects
* Mean 24h arterial blood pressure after the wash-out period: systolic \>180 mmHg and/or diastolic \>110 mmHg
* Secondary hypertension
* Significative cardiovascular complications of diabetes
* Cancer or severe systemic, hepatic, pulmonary, cardiovascular or diseases
* Actual treatment with nitrates, acetylcysteine or arginine
* Acetylcysteine hypersensitivity
* Psychiatric disturbs, abuse of drugs or alcohol
* Low compliance
* Absence of written informed consent
Minimum Eligible Age

40 Years

Maximum Eligible Age

70 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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University of Turin, Italy

OTHER

Sponsor Role lead

Responsible Party

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University of Turin

Principal Investigators

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Valentino Martina, MD

Role: PRINCIPAL_INVESTIGATOR

University of Turin, Italy

Locations

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"San Giovanni Battista" Hospital of Turin

Turin, Turin, Italy

Site Status

Countries

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Italy

Other Identifiers

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EUDRACT 2004-004203-38

Identifier Type: -

Identifier Source: org_study_id